Abstract

Evidence is provided for the presence at the physiological temperature of 20°C of a heat shock transcriptor factor, HSF, in the nuclei of P.lividus embryos. This HSF is able to specifically bind in vitro the heat shock element, HSE, of the promoter of the hsp70 gene IV, as suggested by DNA-protein binding reactions and DNAse I protection assays. Upon heat-shock, at the temperature of 31°C, its ability to bind the HSE units becomes much higher. The HSF activated by heat-shock drives in vivo the transcription of the β-galactosidase reporter gene in transgenic sea urchin gastrulae. An ATF-like transcription factor, widely described in other organisms but not at all in sea urchins, is also present in the nuclear extracts and is able to bind the consensus individuated in the hsp70 TV gene promoter.
Lingua originaleEnglish
pagine (da-a)436-441
Numero di pagine6
RivistaBiochemical and Biophysical Research Communications
Volume240
Stato di pubblicazionePublished - 1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Biochemistry

Cita questo

@article{e63cbb09377b4fec9639f7bce7fc9d43,
title = "Sea urchin HSF activity in vitro and in transgenic embryos",
abstract = "Evidence is provided for the presence at the physiological temperature of 20°C of a heat shock transcriptor factor, HSF, in the nuclei of P.lividus embryos. This HSF is able to specifically bind in vitro the heat shock element, HSE, of the promoter of the hsp70 gene IV, as suggested by DNA-protein binding reactions and DNAse I protection assays. Upon heat-shock, at the temperature of 31°C, its ability to bind the HSE units becomes much higher. The HSF activated by heat-shock drives in vivo the transcription of the β-galactosidase reporter gene in transgenic sea urchin gastrulae. An ATF-like transcription factor, widely described in other organisms but not at all in sea urchins, is also present in the nuclear extracts and is able to bind the consensus individuated in the hsp70 TV gene promoter.",
author = "Giovanni Spinelli and Giovanni Giudice and Gabriella Sconzo and Domenico Cascino and Raffaella Melfi and Fabiana Geraci and Rosalia Sirchia",
year = "1997",
language = "English",
volume = "240",
pages = "436--441",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Sea urchin HSF activity in vitro and in transgenic embryos

AU - Spinelli, Giovanni

AU - Giudice, Giovanni

AU - Sconzo, Gabriella

AU - Cascino, Domenico

AU - Melfi, Raffaella

AU - Geraci, Fabiana

AU - Sirchia, Rosalia

PY - 1997

Y1 - 1997

N2 - Evidence is provided for the presence at the physiological temperature of 20°C of a heat shock transcriptor factor, HSF, in the nuclei of P.lividus embryos. This HSF is able to specifically bind in vitro the heat shock element, HSE, of the promoter of the hsp70 gene IV, as suggested by DNA-protein binding reactions and DNAse I protection assays. Upon heat-shock, at the temperature of 31°C, its ability to bind the HSE units becomes much higher. The HSF activated by heat-shock drives in vivo the transcription of the β-galactosidase reporter gene in transgenic sea urchin gastrulae. An ATF-like transcription factor, widely described in other organisms but not at all in sea urchins, is also present in the nuclear extracts and is able to bind the consensus individuated in the hsp70 TV gene promoter.

AB - Evidence is provided for the presence at the physiological temperature of 20°C of a heat shock transcriptor factor, HSF, in the nuclei of P.lividus embryos. This HSF is able to specifically bind in vitro the heat shock element, HSE, of the promoter of the hsp70 gene IV, as suggested by DNA-protein binding reactions and DNAse I protection assays. Upon heat-shock, at the temperature of 31°C, its ability to bind the HSE units becomes much higher. The HSF activated by heat-shock drives in vivo the transcription of the β-galactosidase reporter gene in transgenic sea urchin gastrulae. An ATF-like transcription factor, widely described in other organisms but not at all in sea urchins, is also present in the nuclear extracts and is able to bind the consensus individuated in the hsp70 TV gene promoter.

UR - http://hdl.handle.net/10447/338455

UR - http://www.sciencedirect.com/science/journal/0006291X

M3 - Article

VL - 240

SP - 436

EP - 441

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

ER -