Sea urchin embryos exposed to cadmium as an experimental model for studying the relationship between autophagy and apoptosis

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Abstract

The sea urchin embryo is a suitable model that offers an excellent opportunity to investigate differentdefence strategies activated in stress conditions. We previously showed that cadmium accumulates in adose- and time-dependent manner into embryonic cells, activating different stress and defence mechanisms,including the synthesis of HSPs and the onset of apoptosis and/or autophagy. In this paper weinvestigated the functional relationship between autophagy and apoptosis, evaluating apoptosis signalsin cadmium-exposed Paracentrotus lividus embryos with inhibited autophagy. We found that the inhibitionof autophagy produced the concurrent reduction of apoptosis, suggesting that the two phenomenaare functionally related. Considering the catabolic role of autophagy, an energetic hypothesis to explainthe relationship was evaluated. Using a substrate for ATP production, we found that apoptosis, assessedby TUNEL and cleaved caspase-3 immunocytochemistry, was substantially restored in cadmium-treatedembryos where autophagy was inhibited by 3-Methyladenine. On the basis of these results, we proposethat, autophagy could play a crucial role in stress response of this organism because autophagy couldenergetically contribute to apoptotic execution through its catabolic role
Lingua originaleEnglish
pagine (da-a)47-55
Numero di pagine9
RivistaMarine Environmental Research
Volume1
Stato di pubblicazionePublished - 2014

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autophagy
apoptosis
Cell death
Echinoidea
Cadmium
embryo
cadmium
embryo (animal)
defense mechanism
Adenosinetriphosphate
Paracentrotus lividus
energetics
sea
immunocytochemistry
caspase-3
substrate
defense mechanisms
Substrates
stress response
synthesis

All Science Journal Classification (ASJC) codes

  • Oceanography
  • Aquatic Science
  • Pollution

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title = "Sea urchin embryos exposed to cadmium as an experimental model for studying the relationship between autophagy and apoptosis",
abstract = "The sea urchin embryo is a suitable model that offers an excellent opportunity to investigate differentdefence strategies activated in stress conditions. We previously showed that cadmium accumulates in adose- and time-dependent manner into embryonic cells, activating different stress and defence mechanisms,including the synthesis of HSPs and the onset of apoptosis and/or autophagy. In this paper weinvestigated the functional relationship between autophagy and apoptosis, evaluating apoptosis signalsin cadmium-exposed Paracentrotus lividus embryos with inhibited autophagy. We found that the inhibitionof autophagy produced the concurrent reduction of apoptosis, suggesting that the two phenomenaare functionally related. Considering the catabolic role of autophagy, an energetic hypothesis to explainthe relationship was evaluated. Using a substrate for ATP production, we found that apoptosis, assessedby TUNEL and cleaved caspase-3 immunocytochemistry, was substantially restored in cadmium-treatedembryos where autophagy was inhibited by 3-Methyladenine. On the basis of these results, we proposethat, autophagy could play a crucial role in stress response of this organism because autophagy couldenergetically contribute to apoptotic execution through its catabolic role",
author = "Roberto Chiarelli and Roccheri, {Maria Carmela} and Liana Bosco and Maria Agnello",
year = "2014",
language = "English",
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journal = "Marine Environmental Research",
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T1 - Sea urchin embryos exposed to cadmium as an experimental model for studying the relationship between autophagy and apoptosis

AU - Chiarelli, Roberto

AU - Roccheri, Maria Carmela

AU - Bosco, Liana

AU - Agnello, Maria

PY - 2014

Y1 - 2014

N2 - The sea urchin embryo is a suitable model that offers an excellent opportunity to investigate differentdefence strategies activated in stress conditions. We previously showed that cadmium accumulates in adose- and time-dependent manner into embryonic cells, activating different stress and defence mechanisms,including the synthesis of HSPs and the onset of apoptosis and/or autophagy. In this paper weinvestigated the functional relationship between autophagy and apoptosis, evaluating apoptosis signalsin cadmium-exposed Paracentrotus lividus embryos with inhibited autophagy. We found that the inhibitionof autophagy produced the concurrent reduction of apoptosis, suggesting that the two phenomenaare functionally related. Considering the catabolic role of autophagy, an energetic hypothesis to explainthe relationship was evaluated. Using a substrate for ATP production, we found that apoptosis, assessedby TUNEL and cleaved caspase-3 immunocytochemistry, was substantially restored in cadmium-treatedembryos where autophagy was inhibited by 3-Methyladenine. On the basis of these results, we proposethat, autophagy could play a crucial role in stress response of this organism because autophagy couldenergetically contribute to apoptotic execution through its catabolic role

AB - The sea urchin embryo is a suitable model that offers an excellent opportunity to investigate differentdefence strategies activated in stress conditions. We previously showed that cadmium accumulates in adose- and time-dependent manner into embryonic cells, activating different stress and defence mechanisms,including the synthesis of HSPs and the onset of apoptosis and/or autophagy. In this paper weinvestigated the functional relationship between autophagy and apoptosis, evaluating apoptosis signalsin cadmium-exposed Paracentrotus lividus embryos with inhibited autophagy. We found that the inhibitionof autophagy produced the concurrent reduction of apoptosis, suggesting that the two phenomenaare functionally related. Considering the catabolic role of autophagy, an energetic hypothesis to explainthe relationship was evaluated. Using a substrate for ATP production, we found that apoptosis, assessedby TUNEL and cleaved caspase-3 immunocytochemistry, was substantially restored in cadmium-treatedembryos where autophagy was inhibited by 3-Methyladenine. On the basis of these results, we proposethat, autophagy could play a crucial role in stress response of this organism because autophagy couldenergetically contribute to apoptotic execution through its catabolic role

UR - http://hdl.handle.net/10447/93436

M3 - Article

VL - 1

SP - 47

EP - 55

JO - Marine Environmental Research

JF - Marine Environmental Research

SN - 0141-1136

ER -