Sabella spallanzanii mucus contain a galactose-binding lectin able to agglutinate bacteria. Purification and characterization

Daniela Piazzese, Maria Giovanna Parisi, Matteo Cammarata, Francesca Falco, Loredana Stabili, Gigliola Benenati

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Abstract

Lectins are present in almost all living organisms and are involved in several biological processes, including immune responses. In the present study, a calcium dependent galactose-binding lectin exhibiting an apparent MW of 43 kDa has been characterized and purified from the mucus of the polychaete Sabella spallanzanii by using both affinity chromatography and high-pressure liquid chromatographic methods. Its agglutinating activity towards rabbit erythrocytes was significantly modified by the addition of calcium or EDTA. The activity was optimal at temperature values comprised between 4 and 18 °C, maintain a 50% of activity between 20 and 37 °C, was significant deleted after exposure at 50 °C, and was depleted at 90 °C. The S. spallanzanii Galactose-Binding Lectin (SsGBL) was able to agglutinate bacteria and to preferentially recognize Gram-negative bacteria. The strongest agglutinating activity was observed towards Vibrio alginolyticus and Escherichia coli, by contrast mucus agglutinated in a lesser extent both Aeromonas hydrophyla and the Gram-positive Micrococcus lysodeikticus thus suggesting its involvement in host pathogen interactions.
Lingua originaleEnglish
pagine (da-a)15-24
Numero di pagine10
RivistaInvertebrate Survival Journal
Volume16
Stato di pubblicazionePublished - 2019

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mucus
lectins
galactose
bacteria
calcium
Vibrio alginolyticus
Micrococcus luteus
Aeromonas
host-pathogen relationships
affinity chromatography
Gram-negative bacteria
Polychaeta
erythrocytes
rabbits
immune response
Escherichia coli
liquids
organisms
temperature
methodology

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

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title = "Sabella spallanzanii mucus contain a galactose-binding lectin able to agglutinate bacteria. Purification and characterization",
abstract = "Lectins are present in almost all living organisms and are involved in several biological processes, including immune responses. In the present study, a calcium dependent galactose-binding lectin exhibiting an apparent MW of 43 kDa has been characterized and purified from the mucus of the polychaete Sabella spallanzanii by using both affinity chromatography and high-pressure liquid chromatographic methods. Its agglutinating activity towards rabbit erythrocytes was significantly modified by the addition of calcium or EDTA. The activity was optimal at temperature values comprised between 4 and 18 °C, maintain a 50{\%} of activity between 20 and 37 °C, was significant deleted after exposure at 50 °C, and was depleted at 90 °C. The S. spallanzanii Galactose-Binding Lectin (SsGBL) was able to agglutinate bacteria and to preferentially recognize Gram-negative bacteria. The strongest agglutinating activity was observed towards Vibrio alginolyticus and Escherichia coli, by contrast mucus agglutinated in a lesser extent both Aeromonas hydrophyla and the Gram-positive Micrococcus lysodeikticus thus suggesting its involvement in host pathogen interactions.",
keywords = "Animal Science and Zoology, Bacteria, Galactose-binding lectin, Hemagglutinin, Mucus, S. spallanzanii",
author = "Daniela Piazzese and Parisi, {Maria Giovanna} and Matteo Cammarata and Francesca Falco and Loredana Stabili and Gigliola Benenati",
year = "2019",
language = "English",
volume = "16",
pages = "15--24",
journal = "Invertebrate Survival Journal",
issn = "1824-307X",
publisher = "University of Modena and Reggio Emilia",

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TY - JOUR

T1 - Sabella spallanzanii mucus contain a galactose-binding lectin able to agglutinate bacteria. Purification and characterization

AU - Piazzese, Daniela

AU - Parisi, Maria Giovanna

AU - Cammarata, Matteo

AU - Falco, Francesca

AU - Stabili, Loredana

AU - Benenati, Gigliola

PY - 2019

Y1 - 2019

N2 - Lectins are present in almost all living organisms and are involved in several biological processes, including immune responses. In the present study, a calcium dependent galactose-binding lectin exhibiting an apparent MW of 43 kDa has been characterized and purified from the mucus of the polychaete Sabella spallanzanii by using both affinity chromatography and high-pressure liquid chromatographic methods. Its agglutinating activity towards rabbit erythrocytes was significantly modified by the addition of calcium or EDTA. The activity was optimal at temperature values comprised between 4 and 18 °C, maintain a 50% of activity between 20 and 37 °C, was significant deleted after exposure at 50 °C, and was depleted at 90 °C. The S. spallanzanii Galactose-Binding Lectin (SsGBL) was able to agglutinate bacteria and to preferentially recognize Gram-negative bacteria. The strongest agglutinating activity was observed towards Vibrio alginolyticus and Escherichia coli, by contrast mucus agglutinated in a lesser extent both Aeromonas hydrophyla and the Gram-positive Micrococcus lysodeikticus thus suggesting its involvement in host pathogen interactions.

AB - Lectins are present in almost all living organisms and are involved in several biological processes, including immune responses. In the present study, a calcium dependent galactose-binding lectin exhibiting an apparent MW of 43 kDa has been characterized and purified from the mucus of the polychaete Sabella spallanzanii by using both affinity chromatography and high-pressure liquid chromatographic methods. Its agglutinating activity towards rabbit erythrocytes was significantly modified by the addition of calcium or EDTA. The activity was optimal at temperature values comprised between 4 and 18 °C, maintain a 50% of activity between 20 and 37 °C, was significant deleted after exposure at 50 °C, and was depleted at 90 °C. The S. spallanzanii Galactose-Binding Lectin (SsGBL) was able to agglutinate bacteria and to preferentially recognize Gram-negative bacteria. The strongest agglutinating activity was observed towards Vibrio alginolyticus and Escherichia coli, by contrast mucus agglutinated in a lesser extent both Aeromonas hydrophyla and the Gram-positive Micrococcus lysodeikticus thus suggesting its involvement in host pathogen interactions.

KW - Animal Science and Zoology

KW - Bacteria

KW - Galactose-binding lectin

KW - Hemagglutinin

KW - Mucus

KW - S. spallanzanii

UR - http://hdl.handle.net/10447/358249

UR - http://www.isj.unimo.it/index.php/ISJ/article/download/478/428

M3 - Article

VL - 16

SP - 15

EP - 24

JO - Invertebrate Survival Journal

JF - Invertebrate Survival Journal

SN - 1824-307X

ER -