RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues

Fabrizio Gianguzza, Maurizio Averna, Angelo Baldassare Cefalu', Maria Antonietta Ragusa, Salvatore Costa, Francesca Fayer, Davide Noto, Salvatore Travali

Risultato della ricerca: Article

9 Citazioni (Scopus)

Abstract

In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.
Lingua originaleEnglish
pagine (da-a)449-455
Numero di pagine7
RivistaInternational Journal of Molecular Medicine
Volume18
Stato di pubblicazionePublished - 2006

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beta 2-Glycoprotein I
In Situ Hybridization
Polymerase Chain Reaction
Proximal Kidney Tubule
Liver
Southern Blotting
Bile Ducts
Northern Blotting
Small Intestine
Prostate
Stomach
Spleen
Epithelial Cells
Kidney
Brain
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

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RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues. / Gianguzza, Fabrizio; Averna, Maurizio; Cefalu', Angelo Baldassare; Ragusa, Maria Antonietta; Costa, Salvatore; Fayer, Francesca; Noto, Davide; Travali, Salvatore.

In: International Journal of Molecular Medicine, Vol. 18, 2006, pag. 449-455.

Risultato della ricerca: Article

Gianguzza, F, Averna, M, Cefalu', AB, Ragusa, MA, Costa, S, Fayer, F, Noto, D & Travali, S 2006, 'RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues', International Journal of Molecular Medicine, vol. 18, pagg. 449-455.
Gianguzza F, Averna M, Cefalu' AB, Ragusa MA, Costa S, Fayer F e altri. RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues. International Journal of Molecular Medicine. 2006;18:449-455.
Gianguzza, Fabrizio ; Averna, Maurizio ; Cefalu', Angelo Baldassare ; Ragusa, Maria Antonietta ; Costa, Salvatore ; Fayer, Francesca ; Noto, Davide ; Travali, Salvatore. / RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues. In: International Journal of Molecular Medicine. 2006 ; Vol. 18. pagg. 449-455.
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abstract = "In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.",
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T1 - RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues

AU - Gianguzza, Fabrizio

AU - Averna, Maurizio

AU - Cefalu', Angelo Baldassare

AU - Ragusa, Maria Antonietta

AU - Costa, Salvatore

AU - Fayer, Francesca

AU - Noto, Davide

AU - Travali, Salvatore

PY - 2006

Y1 - 2006

N2 - In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.

AB - In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.

KW - ß-2-glycoprotein I, apoH, antiphospholipid syndrome, Fanconi syndrome

UR - http://hdl.handle.net/10447/26791

M3 - Article

VL - 18

SP - 449

EP - 455

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

ER -

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