TY - JOUR
T1 - RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues
AU - Ragusa, Maria Antonietta
AU - Averna, Maurizio
AU - Costa, Salvatore
AU - Noto, Davide
AU - Cefalu', Angelo Baldassare
AU - Gianguzza, Fabrizio
AU - Travali, Salvatore
AU - Fayer, Francesca
PY - 2006
Y1 - 2006
N2 - In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.
AB - In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.
KW - Fanconi syndrome
KW - antiphospholipid syndrome
KW - apoH
KW - ß-2-glycoprotein I
KW - Fanconi syndrome
KW - antiphospholipid syndrome
KW - apoH
KW - ß-2-glycoprotein I
UR - http://hdl.handle.net/10447/26791
M3 - Article
VL - 18
SP - 449
EP - 455
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
SN - 1107-3756
ER -