Role(s) of the 5-HT2C receptor in the development of maximal dentate activationin the hippocampus of anesthetized rats.

Arcangelo Benigno, Fabio Bucchieri, Antonella Marino Gammazza, Gergely Orban, Antonella Marino Gammazza, Roberto Colangeli, Philippe De Deurwaerdère, Gergely Orban, Giuseppe Di Giovanni, Cristiano Bombardi, Fabio Bucchieri, Massimo Pierucci, Mauro Pessia, Ilse Smolders, Giuseppe Di Giovanni, Cristoforo Pomara

Risultato della ricerca: Articlepeer review

29 Citazioni (Scopus)


AIMS:Substantial evidence indicates that 5-HT2C receptors are involved in the control of neuronal network excitability and in seizure pathophysiology. Here, we have addressed the relatively unexplored relationship between temporal lobe epilepsy (TLE), the most frequent type of intractable epilepsy, and 5-HT2CRs.METHODS:In this study, we investigated this issue using a model of partial complex (limbic) seizures in urethane-anesthetized rat, based on the phenomenon of maximal dentate activation (MDA) using 5-HT2C compounds, electrophysiology, immunohistochemistry, and western blotting techniques.RESULTS:The 5-HT2C agonists mCPP (1 mg/kg, i.p) and lorcaserin (3 mg/kg, i.p), but not RO60-0175 (1-3 mg/kg i.p.), were antiepileptogenic reducing the MDA response duration. The selective 5-HT2C antagonist SB242084 (2 mg/kg, i.p) unveiled antiepileptogenic effects of RO60-0175 (3 mg/kg, i.p) but did not alter those induced by mCPP and lorcaserin. Compared with control rats, electrically stimulated rats showed an increase in glutamic acid decarboxylase levels and a heterogeneous decrease in 5-HT2CR immunoreactivity in different hippocampal areas.CONCLUSIONS:In our animal model of TLE, mCPP and lorcaserin were anticonvulsant; likely acting on receptor subtypes other than 5-HT2C. Epileptogenesis induced early adaptive changes and reorganization in the 5-HT2CR and GABA systems.
Lingua originaleEnglish
pagine (da-a)651-661
Numero di pagine11
Stato di pubblicazionePublished - 2014

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.2700.2738???
  • ???subjectarea.asjc.2700.2737???
  • ???subjectarea.asjc.2700.2736???


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