Role of secondary metabolites in the biocontrol activity of Pseudomonas corrugata and Pseudomonas mediterranea

Patrizia Bella, Grazia Licciardello, Cinzia Patricia Strano, Vittoria Catara, Andrea Caruso

Risultato della ricerca: Article

2 Citazioni (Scopus)

Abstract

In this study, the Pseudomonas corrugata strain CFBP 5454 and the P. mediterranea strain CFBP 5447 were shown to produce diffusible compounds that inhibit the in vitro growth of plant pathogenic fungi and bacteria and antifungal volatile compounds. In addition, both bacterial strains were found to produce cyanide. Mutant derivatives in LuxR transcriptional regulators, i.e. P. corrugata GL2 (pcoR mutant) and GLRFIA (rfiA mutant), and P. mediterranea PSMER (pmeR mutant) and PSRFIA (rfiA mutant) impaired in cyclic lipopeptide (CLP) production, showed a diffusible compound-mediated reduced activity, depending on the biocontrol strain, challenge microorganism and culture medium. The volatile compound-mediated activity and cyanide production were not affected in the mutants. Genome analysis of the P. corrugata strain CFBP 5454 led to the identification of putative genes involved in the hydrogen cyanide (HCN) biosynthesis. HCN is a volatile organic compound (VOC), and as in other Pseudomonas, the HCN cluster consisted of three contiguous structural genes, hcnABC, which together encoded a membrane-bound HCN synthase complex, which was sufficient for cyanogenesis. The putative hcnA gene was insertionally inactivated. A genomic mutant was characterized, and the role of this compound in biocontrol activity was investigated. A qualitative test for the detection of HCN production confirmed that in the hcnA mutant strain, metabolite production is completely abolished. In vitro experiments on the phytopathogenic fungus Botrytis cinerea also showed that HCN production is mainly involved in conidia germination inhibition.
Lingua originaleEnglish
pagine (da-a)103-115
Numero di pagine13
RivistaEuropean Journal of Plant Pathology
Volume149
Stato di pubblicazionePublished - 2017

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Pseudomonas corrugata
hydrogen cyanide
Pseudomonas
secondary metabolites
biological control
mutants
plant pathogenic fungi
cyanides
volatile compounds
cyanogenesis
lipopeptides
plant pathogenic bacteria
structural genes
volatile organic compounds
Botrytis cinerea
conidia
genes
transcription factors
culture media
chemical derivatives

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science

Cita questo

Role of secondary metabolites in the biocontrol activity of Pseudomonas corrugata and Pseudomonas mediterranea. / Bella, Patrizia; Licciardello, Grazia; Strano, Cinzia Patricia; Catara, Vittoria; Caruso, Andrea.

In: European Journal of Plant Pathology, Vol. 149, 2017, pag. 103-115.

Risultato della ricerca: Article

Bella, Patrizia ; Licciardello, Grazia ; Strano, Cinzia Patricia ; Catara, Vittoria ; Caruso, Andrea. / Role of secondary metabolites in the biocontrol activity of Pseudomonas corrugata and Pseudomonas mediterranea. In: European Journal of Plant Pathology. 2017 ; Vol. 149. pagg. 103-115.
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abstract = "In this study, the Pseudomonas corrugata strain CFBP 5454 and the P. mediterranea strain CFBP 5447 were shown to produce diffusible compounds that inhibit the in vitro growth of plant pathogenic fungi and bacteria and antifungal volatile compounds. In addition, both bacterial strains were found to produce cyanide. Mutant derivatives in LuxR transcriptional regulators, i.e. P. corrugata GL2 (pcoR mutant) and GLRFIA (rfiA mutant), and P. mediterranea PSMER (pmeR mutant) and PSRFIA (rfiA mutant) impaired in cyclic lipopeptide (CLP) production, showed a diffusible compound-mediated reduced activity, depending on the biocontrol strain, challenge microorganism and culture medium. The volatile compound-mediated activity and cyanide production were not affected in the mutants. Genome analysis of the P. corrugata strain CFBP 5454 led to the identification of putative genes involved in the hydrogen cyanide (HCN) biosynthesis. HCN is a volatile organic compound (VOC), and as in other Pseudomonas, the HCN cluster consisted of three contiguous structural genes, hcnABC, which together encoded a membrane-bound HCN synthase complex, which was sufficient for cyanogenesis. The putative hcnA gene was insertionally inactivated. A genomic mutant was characterized, and the role of this compound in biocontrol activity was investigated. A qualitative test for the detection of HCN production confirmed that in the hcnA mutant strain, metabolite production is completely abolished. In vitro experiments on the phytopathogenic fungus Botrytis cinerea also showed that HCN production is mainly involved in conidia germination inhibition.",
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AU - Bella, Patrizia

AU - Licciardello, Grazia

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AU - Caruso, Andrea

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AB - In this study, the Pseudomonas corrugata strain CFBP 5454 and the P. mediterranea strain CFBP 5447 were shown to produce diffusible compounds that inhibit the in vitro growth of plant pathogenic fungi and bacteria and antifungal volatile compounds. In addition, both bacterial strains were found to produce cyanide. Mutant derivatives in LuxR transcriptional regulators, i.e. P. corrugata GL2 (pcoR mutant) and GLRFIA (rfiA mutant), and P. mediterranea PSMER (pmeR mutant) and PSRFIA (rfiA mutant) impaired in cyclic lipopeptide (CLP) production, showed a diffusible compound-mediated reduced activity, depending on the biocontrol strain, challenge microorganism and culture medium. The volatile compound-mediated activity and cyanide production were not affected in the mutants. Genome analysis of the P. corrugata strain CFBP 5454 led to the identification of putative genes involved in the hydrogen cyanide (HCN) biosynthesis. HCN is a volatile organic compound (VOC), and as in other Pseudomonas, the HCN cluster consisted of three contiguous structural genes, hcnABC, which together encoded a membrane-bound HCN synthase complex, which was sufficient for cyanogenesis. The putative hcnA gene was insertionally inactivated. A genomic mutant was characterized, and the role of this compound in biocontrol activity was investigated. A qualitative test for the detection of HCN production confirmed that in the hcnA mutant strain, metabolite production is completely abolished. In vitro experiments on the phytopathogenic fungus Botrytis cinerea also showed that HCN production is mainly involved in conidia germination inhibition.

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