ROC-king onwards: intraepithelial lymphocyte counts, distribution & role in coeliac disease mucosal interpretation

Antonio Carroccio, Gabriel Becheanu, Marie Robert, Mihai Danciu, Mohammad H. Derakhshan, Kamran Ghaffarzadehgan, Sauid Ishaq, Ilaria Russo, Azita Ganji, Andra Neefjes-Borst, Vincenzo Villanacci, Geoffrey Holmes, Roxana Maxim, Kamran Rostami, Matt W. Johnson, Anna Bozzola, Amitabh Srivastava, Sherly Mathews, Calvin Heal, Mohammad Rostami-NejadAlexandra Ciobanu, Mohammad R. Zali, Alessandra Mandolesi, Luca Elli, Masoud Sotoudeh, Brigitte Bancel, Angelo Sidoni, Arzu Ensari, Carolina Ciacci, Carlo Catassi, James J. Going, Umberto Volta, Gabrio Bassotti, Stefano Ferrero, Adrian Bateman, Chris J. Mulder, Michelangelo Fiorentino, Michael N. Marsh, Kamran Ghaffarzadehgan, David Aldulaimi, Hamid Mohaghegh

Risultato della ricerca: Articlepeer review

32 Citazioni (Scopus)


OBJECTIVES:Counting intraepithelial lymphocytes (IEL) is central to the histological diagnosis of coeliac disease (CD), but no definitive 'normal' IEL range has ever been published. In this multicentre study, receiver operating characteristic (ROC) curve analysis was used to determine the optimal cut-off between normal and CD (Marsh III lesion) duodenal mucosa, based on IEL counts on >400 mucosal biopsy specimens.DESIGN:The study was designed at the International Meeting on Digestive Pathology, Bucharest 2015. Investigators from 19 centres, eight countries of three continents, recruited 198 patients with Marsh III histology and 203 controls and used one agreed protocol to count IEL/100 enterocytes in well-oriented duodenal biopsies. Demographic and serological data were also collected.RESULTS:The mean ages of CD and control groups were 45.5 (neonate to 82) and 38.3 (2-88) years. Mean IEL count was 54±18/100 enterocytes in CD and 13±8 in normal controls (p=0.0001). ROC analysis indicated an optimal cut-off point of 25 IEL/100 enterocytes, with 99% sensitivity, 92% specificity and 99.5% area under the curve. Other cut-offs between 20 and 40 IEL were less discriminatory. Additionally, there was a sufficiently high number of biopsies to explore IEL counts across the subclassification of the Marsh III lesion.CONCLUSION:Our ROC curve analyses demonstrate that for Marsh III lesions, a cut-off of 25 IEL/100 enterocytes optimises discrimination between normal control and CD biopsies. No differences in IEL counts were found between Marsh III a, b and c lesions. There was an indication of a continuously graded dose-response by IEL to environmental (gluten) antigenic influence.
Lingua originaleEnglish
pagine (da-a)2080-2086
Numero di pagine7
Stato di pubblicazionePublished - 2017

All Science Journal Classification (ASJC) codes

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