RGD-mimic polyamidoamine-montmorillonite composites with tunable stiffness as scaffolds for bone tissue-engineering applications

Nicolò Mauro, Federica Chiellini, Elisabetta Ranucci, Paolo Ferruti, Michele Laus, Amedea Manfredi, Peter Griffiths, Matteo Gazzarri, Diego Antonioli, Nicolò Mauro, Cristina Bartoli

Risultato della ricerca: Article

9 Citazioni (Scopus)

Abstract

This paper reports on the development of montmorillonite (MMT)-reinforced hydrogels, based on a peptidomimetic polyamidoamine carrying guanidine pendants (AGMA1), as substrates for the osteo-induction of osteoblast precursor cells. AGMA1 hydrogels of various degrees of crosslinking responded favourably to MMT reinforcement, giving rise to composite hydrogels with shear storage modulus G', when fully swollen in water, up to 200 kPa, i.e. 20 times higher than the virgin hydrogels and of the same order or higher than other hydrogel-based composites proposed for orthopaedic applications. This significant improvement was ascribed to the effective interpenetration between the polymer matrix and the inorganic filler. AGMA1-MMT hydrogels, when evaluated as scaffolds for the osteogenic differentiation of mouse calvaria-derived pre-osteoblastic MC3T3-E1 cells, proved able to support cell adhesion and proliferation and clearly induced differentiation towards the osteoblastic phenotype, as indicated by different markers. In addition, AGMA1-MMT hydrogels proved completely degradable in aqueous media at pH 7.4 and did not provide any evidence of cytotoxicity. The experimental evidence suggests that AGMA1-MMT composites definitely warrant potential as scaffolds for osteoblast culture and bone grafts. Copyright © 2016 John Wiley & Sons, Ltd.
Lingua originaleEnglish
pagine (da-a)2164-2175
Numero di pagine12
RivistaJournal of Tissue Engineering and Regenerative Medicine
Volume11
Stato di pubblicazionePublished - 2017

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Bentonite
Hydrogels
Tissue Engineering
Scaffolds (biology)
Clay minerals
Tissue engineering
Bone
Stiffness
Bone and Bones
Composite materials
Osteoblasts
Peptidomimetics
Hydrogel
Cell adhesion
Guanidine
Orthopedics
Cell proliferation
Cytotoxicity
Polymer matrix
Cell culture

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Biomaterials

Cita questo

RGD-mimic polyamidoamine-montmorillonite composites with tunable stiffness as scaffolds for bone tissue-engineering applications. / Mauro, Nicolò; Chiellini, Federica; Ranucci, Elisabetta; Ferruti, Paolo; Laus, Michele; Manfredi, Amedea; Griffiths, Peter; Gazzarri, Matteo; Antonioli, Diego; Mauro, Nicolò; Bartoli, Cristina.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 11, 2017, pag. 2164-2175.

Risultato della ricerca: Article

Mauro, N, Chiellini, F, Ranucci, E, Ferruti, P, Laus, M, Manfredi, A, Griffiths, P, Gazzarri, M, Antonioli, D, Mauro, N & Bartoli, C 2017, 'RGD-mimic polyamidoamine-montmorillonite composites with tunable stiffness as scaffolds for bone tissue-engineering applications', Journal of Tissue Engineering and Regenerative Medicine, vol. 11, pagg. 2164-2175.
Mauro, Nicolò ; Chiellini, Federica ; Ranucci, Elisabetta ; Ferruti, Paolo ; Laus, Michele ; Manfredi, Amedea ; Griffiths, Peter ; Gazzarri, Matteo ; Antonioli, Diego ; Mauro, Nicolò ; Bartoli, Cristina. / RGD-mimic polyamidoamine-montmorillonite composites with tunable stiffness as scaffolds for bone tissue-engineering applications. In: Journal of Tissue Engineering and Regenerative Medicine. 2017 ; Vol. 11. pagg. 2164-2175.
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abstract = "This paper reports on the development of montmorillonite (MMT)-reinforced hydrogels, based on a peptidomimetic polyamidoamine carrying guanidine pendants (AGMA1), as substrates for the osteo-induction of osteoblast precursor cells. AGMA1 hydrogels of various degrees of crosslinking responded favourably to MMT reinforcement, giving rise to composite hydrogels with shear storage modulus G', when fully swollen in water, up to 200 kPa, i.e. 20 times higher than the virgin hydrogels and of the same order or higher than other hydrogel-based composites proposed for orthopaedic applications. This significant improvement was ascribed to the effective interpenetration between the polymer matrix and the inorganic filler. AGMA1-MMT hydrogels, when evaluated as scaffolds for the osteogenic differentiation of mouse calvaria-derived pre-osteoblastic MC3T3-E1 cells, proved able to support cell adhesion and proliferation and clearly induced differentiation towards the osteoblastic phenotype, as indicated by different markers. In addition, AGMA1-MMT hydrogels proved completely degradable in aqueous media at pH 7.4 and did not provide any evidence of cytotoxicity. The experimental evidence suggests that AGMA1-MMT composites definitely warrant potential as scaffolds for osteoblast culture and bone grafts. Copyright {\circledC} 2016 John Wiley & Sons, Ltd.",
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AU - Mauro, Nicolò

AU - Chiellini, Federica

AU - Ranucci, Elisabetta

AU - Ferruti, Paolo

AU - Laus, Michele

AU - Manfredi, Amedea

AU - Griffiths, Peter

AU - Gazzarri, Matteo

AU - Antonioli, Diego

AU - Mauro, Nicolò

AU - Bartoli, Cristina

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N2 - This paper reports on the development of montmorillonite (MMT)-reinforced hydrogels, based on a peptidomimetic polyamidoamine carrying guanidine pendants (AGMA1), as substrates for the osteo-induction of osteoblast precursor cells. AGMA1 hydrogels of various degrees of crosslinking responded favourably to MMT reinforcement, giving rise to composite hydrogels with shear storage modulus G', when fully swollen in water, up to 200 kPa, i.e. 20 times higher than the virgin hydrogels and of the same order or higher than other hydrogel-based composites proposed for orthopaedic applications. This significant improvement was ascribed to the effective interpenetration between the polymer matrix and the inorganic filler. AGMA1-MMT hydrogels, when evaluated as scaffolds for the osteogenic differentiation of mouse calvaria-derived pre-osteoblastic MC3T3-E1 cells, proved able to support cell adhesion and proliferation and clearly induced differentiation towards the osteoblastic phenotype, as indicated by different markers. In addition, AGMA1-MMT hydrogels proved completely degradable in aqueous media at pH 7.4 and did not provide any evidence of cytotoxicity. The experimental evidence suggests that AGMA1-MMT composites definitely warrant potential as scaffolds for osteoblast culture and bone grafts. Copyright © 2016 John Wiley & Sons, Ltd.

AB - This paper reports on the development of montmorillonite (MMT)-reinforced hydrogels, based on a peptidomimetic polyamidoamine carrying guanidine pendants (AGMA1), as substrates for the osteo-induction of osteoblast precursor cells. AGMA1 hydrogels of various degrees of crosslinking responded favourably to MMT reinforcement, giving rise to composite hydrogels with shear storage modulus G', when fully swollen in water, up to 200 kPa, i.e. 20 times higher than the virgin hydrogels and of the same order or higher than other hydrogel-based composites proposed for orthopaedic applications. This significant improvement was ascribed to the effective interpenetration between the polymer matrix and the inorganic filler. AGMA1-MMT hydrogels, when evaluated as scaffolds for the osteogenic differentiation of mouse calvaria-derived pre-osteoblastic MC3T3-E1 cells, proved able to support cell adhesion and proliferation and clearly induced differentiation towards the osteoblastic phenotype, as indicated by different markers. In addition, AGMA1-MMT hydrogels proved completely degradable in aqueous media at pH 7.4 and did not provide any evidence of cytotoxicity. The experimental evidence suggests that AGMA1-MMT composites definitely warrant potential as scaffolds for osteoblast culture and bone grafts. Copyright © 2016 John Wiley & Sons, Ltd.

KW - bone regeneration; composite; montmorillonite; osteo-inductive; polyamidoamines

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