Retrospective Proteomic Screening of 100 Breast Cancer Tissues

Patrizia Cancemi, Ida Pucci, Elena Roz, Nadia Ninfa Albanese, Rosa Musso, Salvatore Minafra

Risultato della ricerca: Article

4 Citazioni (Scopus)

Abstract

The present investigation has been conducted on one hundred tissue fragments of breast cancer, collected and immediately cryopreserved following the surgical resection. The specimens were selected from patients with invasive ductal carcinoma of the breast, the most frequent and potentially aggressive type of mammary cancer, with the objective to increase the knowledge of breast cancer molecular markers potentially useful for clinical applications. The proteomic screening; by 2D-IPG and mass spectrometry; allowed us to identify two main classes of protein clusters: proteins expressed ubiquitously at high levels in all patients; and proteins expressed sporadically among the same patients. Within the group of ubiquitous proteins, glycolytic enzymes and proteins with anti-apoptotic activity were predominant. Among the sporadic ones, proteins involved in cell motility, molecular chaperones and proteins involved in the detoxification appeared prevalent. The data of the present study indicates that the primary tumor growth is reasonably supported by concurrent events: the inhibition of apoptosis and stimulation of cellular proliferation, and the increased expression of glycolytic enzymes with multiple functions. The second phase of the evolution of the tumor can be prematurely scheduled by the occasional presence of proteins involved in cell motility and in the defenses of the oxidative stress. We suggest that this approach on large-scale 2D-IPG proteomics of breast cancer is currently a valid tool that offers the opportunity to evaluate on the same assay the presence and recurrence of individual proteins, their isoforms and short forms, to be proposed as prognostic indicators and susceptibility to metastasis in patients operated on for invasive ductal carcinoma of the breast.
Lingua originaleEnglish
Numero di pagine31
RivistaDefault journal
Volume5 (3)
Stato di pubblicazionePublished - 2017

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Proteomics
Screening
Tissue
Breast Neoplasms
Proteins
Carcinoma, Ductal, Breast
Cell Movement
Tumors
Apoptosis Regulatory Proteins
Molecular Chaperones
Detoxification
Enzymes
Oxidative stress
Mass Spectrometry
Neoplasms
Protein Isoforms
Oxidative Stress
Mass spectrometry
Assays
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry
  • Structural Biology

Cita questo

Cancemi, P., Pucci, I., Roz, E., Albanese, N. N., Musso, R., & Minafra, S. (2017). Retrospective Proteomic Screening of 100 Breast Cancer Tissues. Default journal, 5 (3).

Retrospective Proteomic Screening of 100 Breast Cancer Tissues. / Cancemi, Patrizia; Pucci, Ida; Roz, Elena; Albanese, Nadia Ninfa; Musso, Rosa; Minafra, Salvatore.

In: Default journal, Vol. 5 (3), 2017.

Risultato della ricerca: Article

Cancemi, P, Pucci, I, Roz, E, Albanese, NN, Musso, R & Minafra, S 2017, 'Retrospective Proteomic Screening of 100 Breast Cancer Tissues', Default journal, vol. 5 (3).
Cancemi, Patrizia ; Pucci, Ida ; Roz, Elena ; Albanese, Nadia Ninfa ; Musso, Rosa ; Minafra, Salvatore. / Retrospective Proteomic Screening of 100 Breast Cancer Tissues. In: Default journal. 2017 ; Vol. 5 (3).
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N2 - The present investigation has been conducted on one hundred tissue fragments of breast cancer, collected and immediately cryopreserved following the surgical resection. The specimens were selected from patients with invasive ductal carcinoma of the breast, the most frequent and potentially aggressive type of mammary cancer, with the objective to increase the knowledge of breast cancer molecular markers potentially useful for clinical applications. The proteomic screening; by 2D-IPG and mass spectrometry; allowed us to identify two main classes of protein clusters: proteins expressed ubiquitously at high levels in all patients; and proteins expressed sporadically among the same patients. Within the group of ubiquitous proteins, glycolytic enzymes and proteins with anti-apoptotic activity were predominant. Among the sporadic ones, proteins involved in cell motility, molecular chaperones and proteins involved in the detoxification appeared prevalent. The data of the present study indicates that the primary tumor growth is reasonably supported by concurrent events: the inhibition of apoptosis and stimulation of cellular proliferation, and the increased expression of glycolytic enzymes with multiple functions. The second phase of the evolution of the tumor can be prematurely scheduled by the occasional presence of proteins involved in cell motility and in the defenses of the oxidative stress. We suggest that this approach on large-scale 2D-IPG proteomics of breast cancer is currently a valid tool that offers the opportunity to evaluate on the same assay the presence and recurrence of individual proteins, their isoforms and short forms, to be proposed as prognostic indicators and susceptibility to metastasis in patients operated on for invasive ductal carcinoma of the breast.

AB - The present investigation has been conducted on one hundred tissue fragments of breast cancer, collected and immediately cryopreserved following the surgical resection. The specimens were selected from patients with invasive ductal carcinoma of the breast, the most frequent and potentially aggressive type of mammary cancer, with the objective to increase the knowledge of breast cancer molecular markers potentially useful for clinical applications. The proteomic screening; by 2D-IPG and mass spectrometry; allowed us to identify two main classes of protein clusters: proteins expressed ubiquitously at high levels in all patients; and proteins expressed sporadically among the same patients. Within the group of ubiquitous proteins, glycolytic enzymes and proteins with anti-apoptotic activity were predominant. Among the sporadic ones, proteins involved in cell motility, molecular chaperones and proteins involved in the detoxification appeared prevalent. The data of the present study indicates that the primary tumor growth is reasonably supported by concurrent events: the inhibition of apoptosis and stimulation of cellular proliferation, and the increased expression of glycolytic enzymes with multiple functions. The second phase of the evolution of the tumor can be prematurely scheduled by the occasional presence of proteins involved in cell motility and in the defenses of the oxidative stress. We suggest that this approach on large-scale 2D-IPG proteomics of breast cancer is currently a valid tool that offers the opportunity to evaluate on the same assay the presence and recurrence of individual proteins, their isoforms and short forms, to be proposed as prognostic indicators and susceptibility to metastasis in patients operated on for invasive ductal carcinoma of the breast.

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