Retinol oxidation to retinoic acid in human thyroid glandular cells

Gennaro Taibi, Concetta Nicotra, Giuseppe Carruba, Concetta M. A. Nicotra, Letizia Cocciadiferro, Giuseppe Carruba, Letizia Cocciadiferro, Maria Concetta Gueli

    Risultato della ricerca: Article

    Abstract

    Retinoic acid is regarded as the retinol metabolite that controls proliferation and differentiationof epithelial cells. In the present study, we investigated the potential role of xanthinedehydrogenase (XDH) in retinoic acid biosynthesis in human thyroid glandular cells (HTGC).In particular, we observed that cellular retinoids binding proteins (CRBPs) are also implicatedin the biosynthetic pathway leading to retinoic acid formation in primary cultures of HTGC,as we have already reported for human mammary epithelial cells (HMEC). After partial proteinpurification, the enzyme responsible for retinoic acid biosynthesis was identified and quantifiedas XDH by immunoassay, by its ability to oxidize xanthine to uric acid and its sensitivity to theinhibitory effect of oxypurinol. The evidence of XDH-driven formation of retinoic acid in HTGCcultures further corroborates the potential role of XDH in retinoic acid biosynthesis in theepithelia.
    Lingua originaleEnglish
    pagine (da-a)796-803
    Numero di pagine8
    RivistaJournal of Enzyme Inhibition and Medicinal Chemistry
    Volume29
    Stato di pubblicazionePublished - 2014

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    Tretinoin
    Vitamin A
    Thyroid Gland
    Oxypurinol
    Epithelial Cells
    Retinol-Binding Proteins
    Xanthine
    Biosynthetic Pathways
    Uric Acid
    Immunoassay
    Breast
    Enzymes

    All Science Journal Classification (ASJC) codes

    • Pharmacology
    • Drug Discovery

    Cita questo

    Taibi, G., Nicotra, C., Carruba, G., Nicotra, C. M. A., Cocciadiferro, L., Carruba, G., ... Gueli, M. C. (2014). Retinol oxidation to retinoic acid in human thyroid glandular cells. Journal of Enzyme Inhibition and Medicinal Chemistry, 29, 796-803.

    Retinol oxidation to retinoic acid in human thyroid glandular cells. / Taibi, Gennaro; Nicotra, Concetta; Carruba, Giuseppe; Nicotra, Concetta M. A.; Cocciadiferro, Letizia; Carruba, Giuseppe; Cocciadiferro, Letizia; Gueli, Maria Concetta.

    In: Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 29, 2014, pag. 796-803.

    Risultato della ricerca: Article

    Taibi, G, Nicotra, C, Carruba, G, Nicotra, CMA, Cocciadiferro, L, Carruba, G, Cocciadiferro, L & Gueli, MC 2014, 'Retinol oxidation to retinoic acid in human thyroid glandular cells', Journal of Enzyme Inhibition and Medicinal Chemistry, vol. 29, pagg. 796-803.
    Taibi, Gennaro ; Nicotra, Concetta ; Carruba, Giuseppe ; Nicotra, Concetta M. A. ; Cocciadiferro, Letizia ; Carruba, Giuseppe ; Cocciadiferro, Letizia ; Gueli, Maria Concetta. / Retinol oxidation to retinoic acid in human thyroid glandular cells. In: Journal of Enzyme Inhibition and Medicinal Chemistry. 2014 ; Vol. 29. pagg. 796-803.
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    abstract = "Retinoic acid is regarded as the retinol metabolite that controls proliferation and differentiationof epithelial cells. In the present study, we investigated the potential role of xanthinedehydrogenase (XDH) in retinoic acid biosynthesis in human thyroid glandular cells (HTGC).In particular, we observed that cellular retinoids binding proteins (CRBPs) are also implicatedin the biosynthetic pathway leading to retinoic acid formation in primary cultures of HTGC,as we have already reported for human mammary epithelial cells (HMEC). After partial proteinpurification, the enzyme responsible for retinoic acid biosynthesis was identified and quantifiedas XDH by immunoassay, by its ability to oxidize xanthine to uric acid and its sensitivity to theinhibitory effect of oxypurinol. The evidence of XDH-driven formation of retinoic acid in HTGCcultures further corroborates the potential role of XDH in retinoic acid biosynthesis in theepithelia.",
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    AU - Taibi, Gennaro

    AU - Nicotra, Concetta

    AU - Carruba, Giuseppe

    AU - Nicotra, Concetta M. A.

    AU - Cocciadiferro, Letizia

    AU - Carruba, Giuseppe

    AU - Cocciadiferro, Letizia

    AU - Gueli, Maria Concetta

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    AB - Retinoic acid is regarded as the retinol metabolite that controls proliferation and differentiationof epithelial cells. In the present study, we investigated the potential role of xanthinedehydrogenase (XDH) in retinoic acid biosynthesis in human thyroid glandular cells (HTGC).In particular, we observed that cellular retinoids binding proteins (CRBPs) are also implicatedin the biosynthetic pathway leading to retinoic acid formation in primary cultures of HTGC,as we have already reported for human mammary epithelial cells (HMEC). After partial proteinpurification, the enzyme responsible for retinoic acid biosynthesis was identified and quantifiedas XDH by immunoassay, by its ability to oxidize xanthine to uric acid and its sensitivity to theinhibitory effect of oxypurinol. The evidence of XDH-driven formation of retinoic acid in HTGCcultures further corroborates the potential role of XDH in retinoic acid biosynthesis in theepithelia.

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