Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 851-857 |
| Numero di pagine | 7 |
| Rivista | Anticancer Research |
| Volume | 24 |
| Stato di pubblicazione | Published - 2004 |
All Science Journal Classification (ASJC) codes
- Cancer Research
- Oncology
Cita questo
Resistance to gemcitabine in a lymphoma cell line resistant to Fas-mediated apoptosis. / D'Alessandro, Natale; Dusonchet, Luisa; Rausa, Luciano; Grimaudo, Stefania; Meli, Maria; Notarbartolo Di Villarosa, Monica; Papoff, Giuliana; Ruberti, Giovina.
In: Anticancer Research, Vol. 24, 2004, pag. 851-857.Risultato della ricerca: Article
}
TY - JOUR
T1 - Resistance to gemcitabine in a lymphoma cell line resistant to Fas-mediated apoptosis
AU - D'Alessandro, Natale
AU - Dusonchet, Luisa
AU - Rausa, Luciano
AU - Grimaudo, Stefania
AU - Meli, Maria
AU - Notarbartolo Di Villarosa, Monica
AU - Papoff, Giuliana
AU - Ruberti, Giovina
PY - 2004
Y1 - 2004
N2 - Background: The T-cell lymphoma cell line HuT78B1, selected for resistance to Fas-mediated apoptosis, resulted unexpectedly resistant to the apoptotic and cytotoxic effects of gemcitabine (dFdC). We investigated whether this resistance was due to the impairment of the Fas/Fas-ligand (FasL) system. Materials and Methods: dFdC effects were studied in HuT78B1 and in the parental Fas-sensitive HuT78 cells exposed to inhibitors of the Fas/FasL system. Results: FasL- and Fas-blocking antibodies did not interfere with dFdC-induced apoptosis in HuT78 cells, whereas inhibitors of caspase-8, -9, -1 or -3 had partial inhibitory effects. Notably, in HuT78B1 cells there was a markedly reduced dFdC accumulation notwithstanding a high activity of the activating enzyme deoxycytidine kinase. dFdC accumulation in HuT78 cells was unaffected by a Fas-blocking antibody. Conclusion: This is the first time that the selection of a Fas-resistant cell line led to the isolation of a cell clone unable to accumulate the deoxycytidine analog dFdC. Our results show that this alteration is independent from the impairment of the Fas/FasL system.
AB - Background: The T-cell lymphoma cell line HuT78B1, selected for resistance to Fas-mediated apoptosis, resulted unexpectedly resistant to the apoptotic and cytotoxic effects of gemcitabine (dFdC). We investigated whether this resistance was due to the impairment of the Fas/Fas-ligand (FasL) system. Materials and Methods: dFdC effects were studied in HuT78B1 and in the parental Fas-sensitive HuT78 cells exposed to inhibitors of the Fas/FasL system. Results: FasL- and Fas-blocking antibodies did not interfere with dFdC-induced apoptosis in HuT78 cells, whereas inhibitors of caspase-8, -9, -1 or -3 had partial inhibitory effects. Notably, in HuT78B1 cells there was a markedly reduced dFdC accumulation notwithstanding a high activity of the activating enzyme deoxycytidine kinase. dFdC accumulation in HuT78 cells was unaffected by a Fas-blocking antibody. Conclusion: This is the first time that the selection of a Fas-resistant cell line led to the isolation of a cell clone unable to accumulate the deoxycytidine analog dFdC. Our results show that this alteration is independent from the impairment of the Fas/FasL system.
UR - http://hdl.handle.net/10447/14028
M3 - Article
VL - 24
SP - 851
EP - 857
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
ER -