The aim of this study was to evaluate the ability to resist disinfection of a polymicrobial association contaminatingthe surface of dental impressions obtained with two different elastomers: a polyether (Impregum) and an additionpolymerizedsilicone (Elite). Impressions were contaminated with a mixture of three biofilm-forming microorganisms(Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans) and disinfected immediately after contamination,or after microbial layers were allowed to develop during a six-hour storage. Two commercial disinfectantswere tested: MD 520 containing 0.5% glutaraldehyde and Sterigum Powder without glutaraldehyde. Residual contaminationwas recovered by mechanical rinsing immediately after disinfection and after a six-hour storage of disinfectedimpressions, and assessed by colony counting. Both disinfectants tested were shown to be effective in reducingthe microbial presence on the impression materials, achieving at least a 102 reduction of microbial counts comparedto water rinsing. However, Sterigum was generally less effective on the Elite elastomer and could not grant disinfectionon six-hour aged P. aeruginosa and C. albicans microbial layers. The results of this study suggest that the materialsused for the impressions influence the efficacy of disinfection. Disinfectants should be tested according to conditionsencountered in everyday clinical practice and the need for immediate disinfection of impressions should beclearly indicated by manufacturers.
|Numero di pagine||6|
|Stato di pubblicazione||Published - 2009|
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