Removal of old protein layers from surfaces of works of artby new enzymes

Risultato della ricerca: Other


The aim of the present project is to set up bio-cleaning protocols in order to easily remove altered proteinlayers (e.g. animal glues) by enzymatic proteins (proteases).Protein molecules were isolated from marine invertebrate organisms, characterized by size exclusion highpressureliquid chromatography (Waters: SEC-HPLC, BioSuite 250 to 10㎛ SEC 7.5 x 300 mm) and theirgelatinase activity analyzed by zymography on polyacrilamide gel. The remarkably proteolytic activity and thereaction temperature range of these enzymes, 4° to 37℃, made it possible to hypothesize their use in bioremediationprojects. Tests were performed on protein coating found on some polychrome wooden artifactsexposed to different environmental conditions (temperature, humidity, UV radiation). Particularly, alteredanimal glue layers were removed by using these enzymes at“ room temperature” (19-25.5℃) without changingthe temperature of the surface or of the enzyme solution. The results of the applications were satisfying sincethe removal of the protein layers was carried out in controlled conditions, conducted at the same temperatureas that of the environments where the objects were exposed/stored. In conclusion, the hypothesis is that these enzymes will improve bio-cleaning efficiency even when used atlow temperature instead of commercially available proteases, which are usually active at ≥ 37℃, thusrespecting the aim of conservative restoration.
Lingua originaleEnglish
Numero di pagine2
Stato di pubblicazionePublished - 2012


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