TY - JOUR
T1 - Relevance of 3d culture systems to study osteosarcoma environment
AU - Alessandro, Riccardo
AU - Bellavia, Daniele
AU - Salamanna, Francesca
AU - De Luca, Angela
AU - Carina, Valeria
AU - Costa, Viviana
AU - Alessandro, Riccardo
AU - Giavaresi, Gianluca
AU - Raimondi, Lavinia
AU - Fini, Milena
PY - 2018
Y1 - 2018
N2 - Osteosarcoma (OS) is the most common primary malignant tumor of bone, which preferentially develops lung metastasis. Although standard chemotherapy has significantly improved long-term survival over the past few decades, the outcome for patients with metastatic or recurrent OS remains dramatically poor. Novel therapies are therefore required to slow progression and eradicate the disease. Furthermore, to better understand the cellular and molecular mechanisms responsible for OS onset and progression, the development of novel predictive culture systems resembling the native three-dimensional (3D) tumor microenvironment are mandatory. 'Tumor engineering' approaches radically changed the previous scenario, through the development of advanced and alternative 3D cell culture in vitro models able to tightly mimic the in vivo tumor microenvironment.In this review, we will summarize the state of the art in this novel area, illustrating the different methods and techniques employed to realize 3D OS cell culture models and we report the achieved results, which highlight the efficacy of these models in reproducing the tumor milieu. Although data need to be further validated, the scientific studies reviewed here are certainly promising and give new insights into the clinical practice.
AB - Osteosarcoma (OS) is the most common primary malignant tumor of bone, which preferentially develops lung metastasis. Although standard chemotherapy has significantly improved long-term survival over the past few decades, the outcome for patients with metastatic or recurrent OS remains dramatically poor. Novel therapies are therefore required to slow progression and eradicate the disease. Furthermore, to better understand the cellular and molecular mechanisms responsible for OS onset and progression, the development of novel predictive culture systems resembling the native three-dimensional (3D) tumor microenvironment are mandatory. 'Tumor engineering' approaches radically changed the previous scenario, through the development of advanced and alternative 3D cell culture in vitro models able to tightly mimic the in vivo tumor microenvironment.In this review, we will summarize the state of the art in this novel area, illustrating the different methods and techniques employed to realize 3D OS cell culture models and we report the achieved results, which highlight the efficacy of these models in reproducing the tumor milieu. Although data need to be further validated, the scientific studies reviewed here are certainly promising and give new insights into the clinical practice.
KW - 3D cell culture system
KW - Osteosarcoma
KW - Scaffolds
KW - Spheroids
KW - 3D cell culture system
KW - Osteosarcoma
KW - Scaffolds
KW - Spheroids
UR - http://hdl.handle.net/10447/296843
M3 - Article
VL - 37
SP - 2
EP - 16
JO - JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
JF - JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
SN - 1756-9966
ER -