Relaxation induced by N-terminal fragments of chromogranin A in mouse gastric preparations.

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Abstract

A definitive role for chromogranin A (CGA)-derived fragments in the control of the gastrointestinal smooth muscle contractility has not beenyet established. The purpose of the present study was to evaluate, in vitro, the effects of the recombinant vasostatin 1–78 (VS-1), CGA 7–57 andCGA 47–66 on the mouse gastric mechanical activity, recording the changes of intraluminal pressure. VS-1, CGA 7–57 and CGA 47–66produced concentration-dependent relaxations. Mouse anti-vasostatin-1 monoclonal antibody 5A8, recognising the region 53–57, abolished therelaxation induced by VS-1, indicating the specificity of the effect. The relaxation was significantly reduced by tetrodotoxin (TTX), blocker ofneuronal voltage-dependent Na+ channels, L-NAME, inhibitor of nitric oxide (NO) synthase, or apamin, blocker of small conductance Ca2+-dependent K+ channels. The joint application of TTX and L-NAME did not show any additive effects, whereas TTX plus apamin abolished theVS-1 response. The results suggest that the N-terminal CGA-derived peptides are able to relax mouse gastric muscle and, therefore, they point outan inhibitory role of vasostatin I in the gastrointestinal tract. The relaxation is mediated in part by neural mechanisms through NO production andin part by non-neural mechanisms involving the opening of small conductance Ca2+-dependent K+ channels.
Lingua originaleEnglish
pagine (da-a)90-95
Numero di pagine6
RivistaRegulatory Peptides
Volume139
Stato di pubblicazionePublished - 2007

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Chromogranin A
Stomach
Tetrodotoxin
Apamin
NG-Nitroarginine Methyl Ester
Muscle
Nitric Oxide Synthase
Smooth Muscle
Gastrointestinal Tract
Nitric Oxide
Monoclonal Antibodies
Pressure
Muscles
Peptides
Electric potential
vasostatin

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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@article{d9e92b076ae6432597ead95faee189a8,
title = "Relaxation induced by N-terminal fragments of chromogranin A in mouse gastric preparations.",
abstract = "A definitive role for chromogranin A (CGA)-derived fragments in the control of the gastrointestinal smooth muscle contractility has not beenyet established. The purpose of the present study was to evaluate, in vitro, the effects of the recombinant vasostatin 1–78 (VS-1), CGA 7–57 andCGA 47–66 on the mouse gastric mechanical activity, recording the changes of intraluminal pressure. VS-1, CGA 7–57 and CGA 47–66produced concentration-dependent relaxations. Mouse anti-vasostatin-1 monoclonal antibody 5A8, recognising the region 53–57, abolished therelaxation induced by VS-1, indicating the specificity of the effect. The relaxation was significantly reduced by tetrodotoxin (TTX), blocker ofneuronal voltage-dependent Na+ channels, L-NAME, inhibitor of nitric oxide (NO) synthase, or apamin, blocker of small conductance Ca2+-dependent K+ channels. The joint application of TTX and L-NAME did not show any additive effects, whereas TTX plus apamin abolished theVS-1 response. The results suggest that the N-terminal CGA-derived peptides are able to relax mouse gastric muscle and, therefore, they point outan inhibitory role of vasostatin I in the gastrointestinal tract. The relaxation is mediated in part by neural mechanisms through NO production andin part by non-neural mechanisms involving the opening of small conductance Ca2+-dependent K+ channels.",
author = "Flavia Mule' and Serio, {Rosa Maria} and Antonella Amato",
year = "2007",
language = "English",
volume = "139",
pages = "90--95",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",

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TY - JOUR

T1 - Relaxation induced by N-terminal fragments of chromogranin A in mouse gastric preparations.

AU - Mule', Flavia

AU - Serio, Rosa Maria

AU - Amato, Antonella

PY - 2007

Y1 - 2007

N2 - A definitive role for chromogranin A (CGA)-derived fragments in the control of the gastrointestinal smooth muscle contractility has not beenyet established. The purpose of the present study was to evaluate, in vitro, the effects of the recombinant vasostatin 1–78 (VS-1), CGA 7–57 andCGA 47–66 on the mouse gastric mechanical activity, recording the changes of intraluminal pressure. VS-1, CGA 7–57 and CGA 47–66produced concentration-dependent relaxations. Mouse anti-vasostatin-1 monoclonal antibody 5A8, recognising the region 53–57, abolished therelaxation induced by VS-1, indicating the specificity of the effect. The relaxation was significantly reduced by tetrodotoxin (TTX), blocker ofneuronal voltage-dependent Na+ channels, L-NAME, inhibitor of nitric oxide (NO) synthase, or apamin, blocker of small conductance Ca2+-dependent K+ channels. The joint application of TTX and L-NAME did not show any additive effects, whereas TTX plus apamin abolished theVS-1 response. The results suggest that the N-terminal CGA-derived peptides are able to relax mouse gastric muscle and, therefore, they point outan inhibitory role of vasostatin I in the gastrointestinal tract. The relaxation is mediated in part by neural mechanisms through NO production andin part by non-neural mechanisms involving the opening of small conductance Ca2+-dependent K+ channels.

AB - A definitive role for chromogranin A (CGA)-derived fragments in the control of the gastrointestinal smooth muscle contractility has not beenyet established. The purpose of the present study was to evaluate, in vitro, the effects of the recombinant vasostatin 1–78 (VS-1), CGA 7–57 andCGA 47–66 on the mouse gastric mechanical activity, recording the changes of intraluminal pressure. VS-1, CGA 7–57 and CGA 47–66produced concentration-dependent relaxations. Mouse anti-vasostatin-1 monoclonal antibody 5A8, recognising the region 53–57, abolished therelaxation induced by VS-1, indicating the specificity of the effect. The relaxation was significantly reduced by tetrodotoxin (TTX), blocker ofneuronal voltage-dependent Na+ channels, L-NAME, inhibitor of nitric oxide (NO) synthase, or apamin, blocker of small conductance Ca2+-dependent K+ channels. The joint application of TTX and L-NAME did not show any additive effects, whereas TTX plus apamin abolished theVS-1 response. The results suggest that the N-terminal CGA-derived peptides are able to relax mouse gastric muscle and, therefore, they point outan inhibitory role of vasostatin I in the gastrointestinal tract. The relaxation is mediated in part by neural mechanisms through NO production andin part by non-neural mechanisms involving the opening of small conductance Ca2+-dependent K+ channels.

UR - http://hdl.handle.net/10447/7733

M3 - Article

VL - 139

SP - 90

EP - 95

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

ER -