Reinterpreting a community outbreak of Salmonella enterica serotype Enteritidisin the light of molecular typing

Caterina Mammina, Cristina Romani, Antonino Nastasi, Maria Ida Buonomini, Pierluigi Nicoletti

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    4 Citazioni (Scopus)

    Abstract

    Background: In November 2005, a large outbreak due to Salmonella enterica serotype Enteritidis(S. Enteritidis) was observed within children who had eaten their meals at 53 school cafeterias inFlorence and the surrounding area. A total of 154 isolates of S. Enteritidis were recovered fromhuman cases between November 2005 and January 2006. All strains were assigned phage type 8(PT8) and a common XbaI pulsotype.This paper reports the findings of a molecular epidemiological investigation performed on 124strains of S. Enteritidis isolated in the years 2005 and 2006 in Florence and the surrounding area,including the epidemic isolates.Methods: One hundred twenty-four human isolates of S. Enteritidis identified in the periodJanuary 2005 – December 2006 were submitted to molecular typing by single enzyme – amplifiedfragment length polymorphism (SE-AFLP).Results: Molecular subtyping by SE-AFLP yielded five different profiles. In the pre-epidemic phase,type A included 78.4% of isolates, whereas only three (8.1%) belonged to type C. All isolates, butone, of the epidemic phase were indistinguishable and attributed to type C. In the post-epidemicperiod, a polymorphic pattern of SE-AFLP types was again recognized but type C accounted for73.3% of the isolates during the first six months of 2006, whereas during the remaining six monthstype A regained the first place, including 52.0% of the isolates.Conclusion: The epidemic event was attributed to the emergence and clonal expansion of a strainof S. Enteritidis PT8-SE-AFLP type C. Circulation of the epidemic clone was much more extensivethan the surveillance and traditional laboratory data demonstrated.
    Lingua originaleEnglish
    Numero di pagine6
    RivistaBMC Public Health
    Volume7
    Stato di pubblicazionePublished - 2007

    All Science Journal Classification (ASJC) codes

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