Rapid differentiation between livestock-associated and livestock-independent Staphylococcus aureus CC398 clades

Caterina Mammina, Judy N. Jiménez, Marc Stegger, Frederic Laurent, Alex Van Belkum, Cindy M. Liu, Maliha Aziz, Tania Contente-Cuomo, Stien Vandendriessche, Paal S. Andersen, Katerina Soldanova, Saara Salmenlinna, Jesper Larsen, Robert L. Skov, Anders R. Larsen, Lance B. Price, Andreas Petersen

    Risultato della ricerca: Articlepeer review

    56 Citazioni (Scopus)


    Staphylococcus aureus clonal complex 398 (CC398) isolates cluster into two distinct phylogenetic clades based on single-nucleotide polymorphisms (SNPs) revealing a basal human clade and a more derived livestock clade. The scn and tet(M) genes are strongly associated with the human and the livestock clade, respectively, due to loss and acquisition of mobile genetic elements. We present canonical single-nucleotide polymorphism (canSNP) assays that differentiate the two major host-associated S. aureus CC398 clades and a duplex PCR assay for detection of scn and tet (M). The canSNP assays correctly placed 88 S. aureus CC398 isolates from a reference collection into the human and livestock clades and the duplex PCR assay correctly identified scn and tet(M). The assays were successfully applied to a geographically diverse collection of 272 human S. aureus CC398 isolates. The simple assays described here generate signals comparable to a whole-genome phylogeny for major clade assignment and are easily integrated into S. aureus CC398 surveillance programs and epidemiological studies
    Lingua originaleEnglish
    Numero di pagine5
    RivistaPLoS One
    Stato di pubblicazionePublished - 2013

    All Science Journal Classification (ASJC) codes

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    • ???subjectarea.asjc.1100.1100???
    • ???subjectarea.asjc.1000???


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