The aim of the present study was to analyzeand compare the effects of several metals on the embryosof the sea urchin Paracentrotus lividus, a key specieswithin the Mediterranean Sea ecosystem. Embryos werecontinuously exposed from fertilization to the followingmetals: 0.6 mg/l copper, 3 mg/l lead, and 6 mg/l nickel.The embryos were then monitored for metal responses atthe gastrula stage, which occurred 24 h after exposure. Abiochemical multi-experimental approach was taken andinvolved the investigation of the levels of HSC70 expressionand the involvement of heat shock factor (HSF) and/ormetal transcription factor (MTF) in the response. Immunoblottingassays and electrophoretic mobility shift assays(EMSA) were used to detect stress protein levels and tostudy the interaction between DNA and specific transcriptionfactors, respectively. In the 1 h during exposureto heavy metals, changes in HSC70 levels and HSC70 aphosphorylation state were observed. Rapid changes inHSF and MTF DNA-binding activity also occurred duringthe early stages of heavy metal exposure. In contrast, fewdevelopmental abnormalities were observed at the gastrulastage but more abnormalities were observed 48 h aftermetal exposure. These data demonstrate that changes inHSC70 levels and phosphorylation state as well as in HSFand MTF binding activities may be used to rapidly detectresponses to heavy metal exposure. Detection of biochemicaland molecular changes in response to metalexposure before manifestation of morpho-pathologicaleffects are important for the prediction of morbidity, andthese markers will be useful for determining the response to exposure as part of a toxicological exposure–responseexperiment and for determining responses for an impactassessment.
|Numero di pagine||9|
|Stato di pubblicazione||Published - 2011|
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