Rapid Assessment of Gray Mold (Botrytis cinerea) Infection in Grapes with a Biosensor System

Luciano Cinquanta, Filippo De Curtis, Luciano Cinquanta, Marisa Di Matteo, Alessio Crescitelli, Francesca Malvano, Donatella Albanese

Risultato della ricerca: Article

4 Citazioni (Scopus)

Abstract

Abstract: Botrytis cinerea is the causative agent of gray mold disease, which causes considerable economic losses to winemakers. The extent of gray mold infection of winegrapes is commonly visually estimated, a method that is prone to assessor bias. Here, we used rapid and simple enzyme-based screening consisting of carbon-electrode, screen-printed amperometric biosensors to estimate gluconic acid and glycerol concentration in winegrapes infected with different degrees of B. cinerea. The lower limits of quantification of the screen-printed amperometric biosensors were 3 mg/L for gluconic acid (corresponding to an infection rate of less than 1%) and 35 mg/L for glycerol; the response times with a flow rate of 0.5 mL/min were in a range of 0.5 to 2 min in the linear ranges of the two assays. This study demonstrates the efficacy of amperometric biosensors for rapid analysis of gluconic acid and glycerol in grapes. The measurements confirmed that concentrations of both compounds are highly correlated with the rate of B. cinerea infection (R2 = 0.98). Thus, the biosensor developed to measure gluconic acid in grapes (or must) was more precise and gave a faster response than methods that currently exist for determining the rate of B. cinerea infection of grape berries.
Lingua originaleEnglish
pagine (da-a)502-508
Numero di pagine7
RivistaAmerican Journal of Enology and Viticulture
Volume66
Stato di pubblicazionePublished - 2016

All Science Journal Classification (ASJC) codes

  • Food Science
  • Horticulture

Cita questo

Cinquanta, L., De Curtis, F., Cinquanta, L., Di Matteo, M., Crescitelli, A., Malvano, F., & Albanese, D. (2016). Rapid Assessment of Gray Mold (Botrytis cinerea) Infection in Grapes with a Biosensor System. American Journal of Enology and Viticulture, 66, 502-508.

Rapid Assessment of Gray Mold (Botrytis cinerea) Infection in Grapes with a Biosensor System. / Cinquanta, Luciano; De Curtis, Filippo; Cinquanta, Luciano; Di Matteo, Marisa; Crescitelli, Alessio; Malvano, Francesca; Albanese, Donatella.

In: American Journal of Enology and Viticulture, Vol. 66, 2016, pag. 502-508.

Risultato della ricerca: Article

Cinquanta, L, De Curtis, F, Cinquanta, L, Di Matteo, M, Crescitelli, A, Malvano, F & Albanese, D 2016, 'Rapid Assessment of Gray Mold (Botrytis cinerea) Infection in Grapes with a Biosensor System', American Journal of Enology and Viticulture, vol. 66, pagg. 502-508.
Cinquanta, Luciano ; De Curtis, Filippo ; Cinquanta, Luciano ; Di Matteo, Marisa ; Crescitelli, Alessio ; Malvano, Francesca ; Albanese, Donatella. / Rapid Assessment of Gray Mold (Botrytis cinerea) Infection in Grapes with a Biosensor System. In: American Journal of Enology and Viticulture. 2016 ; Vol. 66. pagg. 502-508.
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abstract = "Abstract: Botrytis cinerea is the causative agent of gray mold disease, which causes considerable economic losses to winemakers. The extent of gray mold infection of winegrapes is commonly visually estimated, a method that is prone to assessor bias. Here, we used rapid and simple enzyme-based screening consisting of carbon-electrode, screen-printed amperometric biosensors to estimate gluconic acid and glycerol concentration in winegrapes infected with different degrees of B. cinerea. The lower limits of quantification of the screen-printed amperometric biosensors were 3 mg/L for gluconic acid (corresponding to an infection rate of less than 1{\%}) and 35 mg/L for glycerol; the response times with a flow rate of 0.5 mL/min were in a range of 0.5 to 2 min in the linear ranges of the two assays. This study demonstrates the efficacy of amperometric biosensors for rapid analysis of gluconic acid and glycerol in grapes. The measurements confirmed that concentrations of both compounds are highly correlated with the rate of B. cinerea infection (R2 = 0.98). Thus, the biosensor developed to measure gluconic acid in grapes (or must) was more precise and gave a faster response than methods that currently exist for determining the rate of B. cinerea infection of grape berries.",
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AU - Cinquanta, Luciano

AU - De Curtis, Filippo

AU - Cinquanta, Luciano

AU - Di Matteo, Marisa

AU - Crescitelli, Alessio

AU - Malvano, Francesca

AU - Albanese, Donatella

PY - 2016

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N2 - Abstract: Botrytis cinerea is the causative agent of gray mold disease, which causes considerable economic losses to winemakers. The extent of gray mold infection of winegrapes is commonly visually estimated, a method that is prone to assessor bias. Here, we used rapid and simple enzyme-based screening consisting of carbon-electrode, screen-printed amperometric biosensors to estimate gluconic acid and glycerol concentration in winegrapes infected with different degrees of B. cinerea. The lower limits of quantification of the screen-printed amperometric biosensors were 3 mg/L for gluconic acid (corresponding to an infection rate of less than 1%) and 35 mg/L for glycerol; the response times with a flow rate of 0.5 mL/min were in a range of 0.5 to 2 min in the linear ranges of the two assays. This study demonstrates the efficacy of amperometric biosensors for rapid analysis of gluconic acid and glycerol in grapes. The measurements confirmed that concentrations of both compounds are highly correlated with the rate of B. cinerea infection (R2 = 0.98). Thus, the biosensor developed to measure gluconic acid in grapes (or must) was more precise and gave a faster response than methods that currently exist for determining the rate of B. cinerea infection of grape berries.

AB - Abstract: Botrytis cinerea is the causative agent of gray mold disease, which causes considerable economic losses to winemakers. The extent of gray mold infection of winegrapes is commonly visually estimated, a method that is prone to assessor bias. Here, we used rapid and simple enzyme-based screening consisting of carbon-electrode, screen-printed amperometric biosensors to estimate gluconic acid and glycerol concentration in winegrapes infected with different degrees of B. cinerea. The lower limits of quantification of the screen-printed amperometric biosensors were 3 mg/L for gluconic acid (corresponding to an infection rate of less than 1%) and 35 mg/L for glycerol; the response times with a flow rate of 0.5 mL/min were in a range of 0.5 to 2 min in the linear ranges of the two assays. This study demonstrates the efficacy of amperometric biosensors for rapid analysis of gluconic acid and glycerol in grapes. The measurements confirmed that concentrations of both compounds are highly correlated with the rate of B. cinerea infection (R2 = 0.98). Thus, the biosensor developed to measure gluconic acid in grapes (or must) was more precise and gave a faster response than methods that currently exist for determining the rate of B. cinerea infection of grape berries.

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M3 - Article

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JO - American Journal of Enology and Viticulture

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SN - 0002-9254

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