Abstract: Botrytis cinerea is the causative agent of gray mold disease, which causes considerable economic lossesto winemakers. The extent of gray mold infection of winegrapes is commonly visually estimated, a method thatis prone to assessor bias. Here, we used rapid and simple enzyme-based screening consisting of carbon-electrode,screen-printed amperometric biosensors to estimate gluconic acid and glycerol concentration in winegrapes infectedwith different degrees of B. cinerea. The lower limits of quantification of the screen-printed amperometric biosensorswere 3 mg/L for gluconic acid (corresponding to an infection rate of less than 1%) and 35 mg/L for glycerol;the response times with a flow rate of 0.5 mL/min were in a range of 0.5 to 2 min in the linear ranges of the twoassays. This study demonstrates the efficacy of amperometric biosensors for rapid analysis of gluconic acid andglycerol in grapes. The measurements confirmed that concentrations of both compounds are highly correlated withthe rate of B. cinerea infection (R2 = 0.98). Thus, the biosensor developed to measure gluconic acid in grapes (ormust) was more precise and gave a faster response than methods that currently exist for determining the rate of B.cinerea infection of grape berries.
|Numero di pagine||7|
|Rivista||American Journal of Enology and Viticulture|
|Stato di pubblicazione||Published - 2016|
All Science Journal Classification (ASJC) codes
- Food Science