Radiation synthesis of nanogels as therapeutic agent vectors

Risultato della ricerca: Otherpeer review


Nanogels (NGs) are physically or chemically crosslinked polymer networks and arepromising candidates in the development of therapeutic agent vectors. In fact,thanks to their tunable size and properties, they offer unique advantages, includinga large and flexible surface for multivalent bio-conjugation, an internal 3D aqueousenvironment for incorporation and protection of (bio)molecular drugs, stimuliresponsivenessto achieve temporal and/or site control of the release function andbiocompatibility.In order to develop effective NGs-based biomedical devices an inexpensive, robustand versatile synthetic methodology is required. In this perspective, we haveproduced NGs with high yields and through-puts by pulsed electron-beamirradiation. In particular, using an industrial electron accelerator, carboxylfunctionalized NGs from a dilute aqueous solutions of poly(N-vinyl pyrrolidone)(PVP), and acrylic acid as functional monomer have been produced. This processallows simultaneous polymer cross-linking and monomer grafting. Moreover,depending on the value of the total irradiation dose, also the sterilization of theirradiated material can be obtained. Since organic solvents, toxic initiators orcatalysts and surfactants are not required, this procedure can be defined as aneco-friendly and clean one.Crosslinked nanoparticles with multi-armed surfaces, and size, crosslinking density,and surface electric charge density controlled, have been generated. Nanogelsproduced have been proven to be hemocompatible and not cytotoxic or genotoxicat the cellular level.NGs have been decorated with fluorescent probes, proteins and anti-MiR. Thanks tothe use of fluorescent variants, it has been argued that NGs showed a good affinityfor cells, as they rapidly and quantitatively bypass the cellular compartments,accumulating in specific cell portions for the first hours, and being completelyreleased from the cells in the next 24 hours. Moreover, it has been proven that theproteins and anti-MiR, once conjugated, maintain their biological activity givingrise to targeting features toward specific cell types.
Lingua originaleEnglish
Numero di pagine2
Stato di pubblicazionePublished - 2015


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