Quantitative immunomorphological analysis of heat shock proteins in thyroid follicular adenoma and carcinoma tissues reveals their potential for differential diagnosis and points to a role in carcinogenesis

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Abstract

Hsp27, Hsp60, Hsp70, and Hsp90 are chaperones that play a crucial role in cellularhomeostasis and differentiation, but they may be implicated in carcinogenesis. Follicular neoplasmsof the thyroid include follicular adenoma and follicular carcinoma. The former is a very frequent benignencapsulated nodule, whereas the other is a nodule that infiltrates the capsule, blood vessels and theadjacent parenchyma, with a tendency to metastasize. The main objective was to assess the potentialof the Hsps in differential diagnosis and carcinogenesis. We quantified by immunohistochemistryHsp27, Hsp60, Hsp70, and Hsp90 on thin sections of human thyroid tissue with follicular adenoma orfollicular carcinoma, comparing the tumor with the adjacent peritumoral tissue. Hsp60, Hsp70, andHsp90 were increased in follicular carcinoma compared to follicular adenoma, while Hsp27 showedno difference. Histochemical quantification of Hsp60, Hsp70, and Hsp90 allows diagnostic distinctionbetween follicular adenoma and carcinoma, and between tumor and adjacent non-tumoral tissue.The quantitative variations of these chaperones in follicular carcinoma suggest their involvement intumorigenesis, for instance in processes such as invasion of thyroid parenchyma and metastasization.
Lingua originaleEnglish
pagine (da-a)4324-
Numero di pagine11
RivistaAPPLIED SCIENCES
Volume9
Stato di pubblicazionePublished - 2019

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Heat-Shock Proteins
quantitative analysis
cancer
shock
Tissue
proteins
Proteins
heat
Tumors
Chemical analysis
nodules
Blood vessels
tumors
Capsules
blood vessels
capsules
tendencies
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

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@article{005c8fda5cd84f52847e92b6a57c741e,
title = "Quantitative immunomorphological analysis of heat shock proteins in thyroid follicular adenoma and carcinoma tissues reveals their potential for differential diagnosis and points to a role in carcinogenesis",
abstract = "Hsp27, Hsp60, Hsp70, and Hsp90 are chaperones that play a crucial role in cellularhomeostasis and differentiation, but they may be implicated in carcinogenesis. Follicular neoplasmsof the thyroid include follicular adenoma and follicular carcinoma. The former is a very frequent benignencapsulated nodule, whereas the other is a nodule that infiltrates the capsule, blood vessels and theadjacent parenchyma, with a tendency to metastasize. The main objective was to assess the potentialof the Hsps in differential diagnosis and carcinogenesis. We quantified by immunohistochemistryHsp27, Hsp60, Hsp70, and Hsp90 on thin sections of human thyroid tissue with follicular adenoma orfollicular carcinoma, comparing the tumor with the adjacent peritumoral tissue. Hsp60, Hsp70, andHsp90 were increased in follicular carcinoma compared to follicular adenoma, while Hsp27 showedno difference. Histochemical quantification of Hsp60, Hsp70, and Hsp90 allows diagnostic distinctionbetween follicular adenoma and carcinoma, and between tumor and adjacent non-tumoral tissue.The quantitative variations of these chaperones in follicular carcinoma suggest their involvement intumorigenesis, for instance in processes such as invasion of thyroid parenchyma and metastasization.",
keywords = "Carcinogenesis, Chaperonopathies, Differential diagnosis, Follicular adenoma, Follicular carcinoma, Hsp27, Hsp60, Hsp70, Hsp90, Molecular chaperone, Thyroid",
author = "Calogero Cipolla and Sabrina David and Giuseppa Graceffa and Francesca Rappa and Alessandro Pitruzzella and Fabio Bucchieri and Francesco Cappello and Vitale, {Alessandra Maria} and Daniela Cabibi and Letizia Paladino and Fucarino, {Alberto Giuseppe} and {De Macario}, {Everly Conway} and Francesco Cappello and Macario, {Alberto J.L.} and Stefania Martorana",
year = "2019",
language = "English",
volume = "9",
pages = "4324--",
journal = "Applied Sciences (Switzerland)",
issn = "2076-3417",
publisher = "Multidisciplinary Digital Publishing Institute",

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TY - JOUR

T1 - Quantitative immunomorphological analysis of heat shock proteins in thyroid follicular adenoma and carcinoma tissues reveals their potential for differential diagnosis and points to a role in carcinogenesis

AU - Cipolla, Calogero

AU - David, Sabrina

AU - Graceffa, Giuseppa

AU - Rappa, Francesca

AU - Pitruzzella, Alessandro

AU - Bucchieri, Fabio

AU - Cappello, Francesco

AU - Vitale, Alessandra Maria

AU - Cabibi, Daniela

AU - Paladino, Letizia

AU - Fucarino, Alberto Giuseppe

AU - De Macario, Everly Conway

AU - Cappello, Francesco

AU - Macario, Alberto J.L.

AU - Martorana, Stefania

PY - 2019

Y1 - 2019

N2 - Hsp27, Hsp60, Hsp70, and Hsp90 are chaperones that play a crucial role in cellularhomeostasis and differentiation, but they may be implicated in carcinogenesis. Follicular neoplasmsof the thyroid include follicular adenoma and follicular carcinoma. The former is a very frequent benignencapsulated nodule, whereas the other is a nodule that infiltrates the capsule, blood vessels and theadjacent parenchyma, with a tendency to metastasize. The main objective was to assess the potentialof the Hsps in differential diagnosis and carcinogenesis. We quantified by immunohistochemistryHsp27, Hsp60, Hsp70, and Hsp90 on thin sections of human thyroid tissue with follicular adenoma orfollicular carcinoma, comparing the tumor with the adjacent peritumoral tissue. Hsp60, Hsp70, andHsp90 were increased in follicular carcinoma compared to follicular adenoma, while Hsp27 showedno difference. Histochemical quantification of Hsp60, Hsp70, and Hsp90 allows diagnostic distinctionbetween follicular adenoma and carcinoma, and between tumor and adjacent non-tumoral tissue.The quantitative variations of these chaperones in follicular carcinoma suggest their involvement intumorigenesis, for instance in processes such as invasion of thyroid parenchyma and metastasization.

AB - Hsp27, Hsp60, Hsp70, and Hsp90 are chaperones that play a crucial role in cellularhomeostasis and differentiation, but they may be implicated in carcinogenesis. Follicular neoplasmsof the thyroid include follicular adenoma and follicular carcinoma. The former is a very frequent benignencapsulated nodule, whereas the other is a nodule that infiltrates the capsule, blood vessels and theadjacent parenchyma, with a tendency to metastasize. The main objective was to assess the potentialof the Hsps in differential diagnosis and carcinogenesis. We quantified by immunohistochemistryHsp27, Hsp60, Hsp70, and Hsp90 on thin sections of human thyroid tissue with follicular adenoma orfollicular carcinoma, comparing the tumor with the adjacent peritumoral tissue. Hsp60, Hsp70, andHsp90 were increased in follicular carcinoma compared to follicular adenoma, while Hsp27 showedno difference. Histochemical quantification of Hsp60, Hsp70, and Hsp90 allows diagnostic distinctionbetween follicular adenoma and carcinoma, and between tumor and adjacent non-tumoral tissue.The quantitative variations of these chaperones in follicular carcinoma suggest their involvement intumorigenesis, for instance in processes such as invasion of thyroid parenchyma and metastasization.

KW - Carcinogenesis

KW - Chaperonopathies

KW - Differential diagnosis

KW - Follicular adenoma

KW - Follicular carcinoma

KW - Hsp27

KW - Hsp60

KW - Hsp70

KW - Hsp90

KW - Molecular chaperone

KW - Thyroid

UR - http://hdl.handle.net/10447/379894

UR - https://res.mdpi.com/d_attachment/applsci/applsci-09-04324/article_deploy/applsci-09-04324.pdf

M3 - Article

VL - 9

SP - 4324-

JO - Applied Sciences (Switzerland)

JF - Applied Sciences (Switzerland)

SN - 2076-3417

ER -