Quantitative determination of casein genetic variants in goat milk: Application in Girgentana dairy goat breed

Montalbano, M; Segreto, R; Maria Teresa Sardina.

Risultato della ricerca: Article

7 Citazioni (Scopus)

Abstract

The study was conducted to develop a high-performance liquid chromatographic (HPLC) method to quantify casein genetic variants (s2-, β-, and κ-casein) in milk of homozygous individuals of Girgentana goat breed. For calibration experiments, pure genetic variants were extracted from individual milk samples of animals with known genotypes. The described HPLC approach was precise, accurate and highly suitable for quantification of goat casein genetic variants of homozygous individuals. The amount of each casein per allele was: s2-casein A=2.9 ± 0.8 g/L and F=1.8 ± 0.4 g/L; β-casein C=3.0 ± 0.8 g/L and C1=2.0 ± 0.7 g/L and κ-casein A=1.6 ± 0.3 g/L and B=1.1 ± 0.2 g/L. A good correlation was found between the quantities of s2-casein genetic variants A and F, and β-casein C and C1 with other previously described method. The main important result was obtained for κ-casein because, till now, no data were available on quantification of single genetic variants for this protein.
Lingua originaleEnglish
pagine (da-a)760-764
Numero di pagine5
RivistaFood Chemistry
Volume192
Stato di pubblicazionePublished - 2016

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dairy breeds
goat breeds
Dairies
dairy goats
goat milk
Caseins
Goats
casein
Milk
kappa-casein
beta-casein
milk
liquids
calibration
goats
Girgentana (goat breed)
alleles
genotype
methodology
Liquids

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine(all)
  • Analytical Chemistry

Cita questo

Quantitative determination of casein genetic variants in goat milk: Application in Girgentana dairy goat breed. / Montalbano, M; Segreto, R; Maria Teresa Sardina.

In: Food Chemistry, Vol. 192, 2016, pag. 760-764.

Risultato della ricerca: Article

Montalbano, M; Segreto, R; Maria Teresa Sardina. 2016, 'Quantitative determination of casein genetic variants in goat milk: Application in Girgentana dairy goat breed', Food Chemistry, vol. 192, pagg. 760-764.
Montalbano, M; Segreto, R; Maria Teresa Sardina. / Quantitative determination of casein genetic variants in goat milk: Application in Girgentana dairy goat breed. In: Food Chemistry. 2016 ; Vol. 192. pagg. 760-764.
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abstract = "The study was conducted to develop a high-performance liquid chromatographic (HPLC) method to quantify casein genetic variants (s2-, β-, and κ-casein) in milk of homozygous individuals of Girgentana goat breed. For calibration experiments, pure genetic variants were extracted from individual milk samples of animals with known genotypes. The described HPLC approach was precise, accurate and highly suitable for quantification of goat casein genetic variants of homozygous individuals. The amount of each casein per allele was: s2-casein A=2.9 ± 0.8 g/L and F=1.8 ± 0.4 g/L; β-casein C=3.0 ± 0.8 g/L and C1=2.0 ± 0.7 g/L and κ-casein A=1.6 ± 0.3 g/L and B=1.1 ± 0.2 g/L. A good correlation was found between the quantities of s2-casein genetic variants A and F, and β-casein C and C1 with other previously described method. The main important result was obtained for κ-casein because, till now, no data were available on quantification of single genetic variants for this protein.",
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AU - Montalbano, M; Segreto, R; Maria Teresa Sardina.

AU - Mastrangelo, Salvatore

AU - Sardina, Maria Teresa

AU - Di Gerlando, Rosalia

PY - 2016

Y1 - 2016

N2 - The study was conducted to develop a high-performance liquid chromatographic (HPLC) method to quantify casein genetic variants (s2-, β-, and κ-casein) in milk of homozygous individuals of Girgentana goat breed. For calibration experiments, pure genetic variants were extracted from individual milk samples of animals with known genotypes. The described HPLC approach was precise, accurate and highly suitable for quantification of goat casein genetic variants of homozygous individuals. The amount of each casein per allele was: s2-casein A=2.9 ± 0.8 g/L and F=1.8 ± 0.4 g/L; β-casein C=3.0 ± 0.8 g/L and C1=2.0 ± 0.7 g/L and κ-casein A=1.6 ± 0.3 g/L and B=1.1 ± 0.2 g/L. A good correlation was found between the quantities of s2-casein genetic variants A and F, and β-casein C and C1 with other previously described method. The main important result was obtained for κ-casein because, till now, no data were available on quantification of single genetic variants for this protein.

AB - The study was conducted to develop a high-performance liquid chromatographic (HPLC) method to quantify casein genetic variants (s2-, β-, and κ-casein) in milk of homozygous individuals of Girgentana goat breed. For calibration experiments, pure genetic variants were extracted from individual milk samples of animals with known genotypes. The described HPLC approach was precise, accurate and highly suitable for quantification of goat casein genetic variants of homozygous individuals. The amount of each casein per allele was: s2-casein A=2.9 ± 0.8 g/L and F=1.8 ± 0.4 g/L; β-casein C=3.0 ± 0.8 g/L and C1=2.0 ± 0.7 g/L and κ-casein A=1.6 ± 0.3 g/L and B=1.1 ± 0.2 g/L. A good correlation was found between the quantities of s2-casein genetic variants A and F, and β-casein C and C1 with other previously described method. The main important result was obtained for κ-casein because, till now, no data were available on quantification of single genetic variants for this protein.

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JF - Food Chemistry

SN - 0308-8146

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