Quantitative analysis of the impact of a human pathogenic mutation on the CCT5 chaperonin subunit using a proxy archaeal ortholog

Francesco Cappello, Dario Spigolon, D. Travis Gallagher, Dario Spigolon, Pier Luigi San Biagio, D. Travis Gallagher, Jatin Narang, Jatin Narang, Adrian Velazquez-Campoy, Donatella Bulone, Francesco Cappello, Frank T. Robb, Everly Conway De Macario, Alberto J.L. Macario, Pier Luigi San Biagio

Risultato della ricerca: Articlepeer review

4 Citazioni (Scopus)

Abstract

The human chaperonin complex is a ~ 1 MDa nanomachine composed of two octameric rings formed from eight similar but non-identical subunits called CCT. Here, we are elucidating the mechanism of a heritable CCT5 subunit mutation that causes profound neuropathy in humans. In previous work, we introduced an equivalent mutation in an archaeal chaperonin that assembles into two octameric rings like in humans but in which all subunits are identical. We reported that the hexadecamer formed by the mutant subunit is unstable with impaired chaperoning functions. This study quantifies the loss of structural stability in the hexadecamer due to the pathogenic mutation, using differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC). The disassembly of the wild type complex, which is tightly coupled with subunit denaturation, was decoupled by the mutation without affecting the stability of individual subunits. Our results verify the effectiveness of the homo-hexadecameric archaeal chaperonin as a proxy to assess the impact of subtle defects in heterologous systems with mutations in a single subunit.
Lingua originaleEnglish
pagine (da-a)66-71
Numero di pagine6
RivistaBiochemistry and Biophysics Reports
Volume12
Stato di pubblicazionePublished - 2017

All Science Journal Classification (ASJC) codes

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