SCOPETo inquire with certainty of the analytical data on the effects of fat acids.INTRODUCTIONThe consumption of trans isomers of unsaturated fatty acids has been associated with untoward metabolic effects.Several clinical investigations demonstrated that trans fatty acids increase plasma LDL-cholesterol and lipoprotein (a) andreduce HDL-cholesterol concentrations. These alterations of plasma lipid profiles indicate an atherogenic effect of transfatty acids. Moreover, in infant and children, trans fatty acids in blood plasma may impair human growth.METHODOLOGYWe have put to point one methodology analytics in order to dose trans fatty acids in the blood. Such methodologyanalytics previews the use of the technology of mass for having the certainty of the analytical data in one complex matrixwhich that one of the blood.The conditions analytics preview: extraction, methylation with acetyl chloride, extraction with Hexane, evaporationunder nitrogen current, to bring back by volume with Hexane, separation gas chromatography, quantization in SCAN byQualifier Ion ratios and SIM identification.Quadrupolar GCMS settings:Column: SPTM-2560; Oven: 140C (5min) to 240C at 4C/min; Carrier: Helium, 20cm/sec; Transfer line: 250CInjector: 1μl, splitless, 260CRESULTSIn a population of approximately 60 healthy adult and 40 healthy children we have found blood plasma arachidonicacid (C20:4)-6) levels and the product/substrate ratios of arachidonic acid synthesis (C20:40)-6/C18:20)-6) inversely correlatedto the level of the principal trans fatty acid, trans octadecaenoic. This fact is of extreme importance.It appears desirable to limit the dietary intake of trans fatty acids. The major part of human trans fatty acid exposure isthe consequence of industrial food processing and the major dietary sources of trans fatty acids are partially hydrogenatedvegetable and fish oils. Refinement of the industrial technology of partial hydrogenation and appropriate food labellingmay lead to a considerably decrease of human exposure to transfatty acids.REFERENCESDemmelmalr H, Festl B, Wolfram G, Koletzko B. Trans fatty acid contents in spreads and cold cuts usually consumedby children.Z Ern~hrungswiss 1996; 35:235-240Koletzko B, Edenhofer S, Lipowsky G, Reinhardt D. Effects of a low birthweight infant formula containing docosahexaenoicand arachidonic acids at human milk levels. J Pediatr Gastroenterol Nutr 1995; 21:200-208
Lingua originaleEnglish
Stato di pubblicazionePublished - 2007

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