TY - JOUR
T1 - PTP1B phosphatase as a novel target of oleuropein activity in MCF-7 breast cancer model
AU - Lo Celso, Fabrizio
AU - Barone, Giampaolo
AU - Lo Bosco, Giosue'
AU - Daca, Agnieszka
AU - Kuban-Jankowska, Alicja
AU - Wyszkowska, Roksana
AU - Gorska-Ponikowska, Magdalena
AU - Przychodzen, Paulina
AU - Kostrzewa, Tomasz
AU - Kamm, Anna
AU - Gorska-Ponikowska, Magdalena
AU - Lo Bosco, Giosuè
AU - Bosco, Giosuè Lo
PY - 2019
Y1 - 2019
N2 - Phosphatase PTP1B has become a therapeutic target for the treatment of type 2-diabetes, whereas recent studies have revealed that PTP1B plays a pivotal role in pathophysiology and development of breast cancer. Oleuropein is a natural, phenolic compound with anticancer activity. The aim of this study was to address the question whether PTP1B constitutes a target for oleuropein in breast cancer MCF-7 cells. The cellular MCF-7 breast cancer model was used in the study. The experiments were performed using cellular viability tests, Elisa assays, immunoprecipitation, flow cytometry analyses and computer modelling. Herein, we evidenced that the reduced activity of phosphatase PTP1B after treatment with oleuropein is strictly correlated with decreased MCF-7 cellular viability and cell cycle arrest. These results provide new insight into further research on oleuropein and possible role of the compound in adjuvant treatment of breast cancer
AB - Phosphatase PTP1B has become a therapeutic target for the treatment of type 2-diabetes, whereas recent studies have revealed that PTP1B plays a pivotal role in pathophysiology and development of breast cancer. Oleuropein is a natural, phenolic compound with anticancer activity. The aim of this study was to address the question whether PTP1B constitutes a target for oleuropein in breast cancer MCF-7 cells. The cellular MCF-7 breast cancer model was used in the study. The experiments were performed using cellular viability tests, Elisa assays, immunoprecipitation, flow cytometry analyses and computer modelling. Herein, we evidenced that the reduced activity of phosphatase PTP1B after treatment with oleuropein is strictly correlated with decreased MCF-7 cellular viability and cell cycle arrest. These results provide new insight into further research on oleuropein and possible role of the compound in adjuvant treatment of breast cancer
UR - http://hdl.handle.net/10447/367184
M3 - Article
VL - 61
SP - 1
EP - 9
JO - Toxicology in Vitro
JF - Toxicology in Vitro
SN - 0887-2333
ER -