Proteomic Profiling of Colon Cancer Tissues: Discovery of New Candidate Biomarkers

Miriam Buttacavoli, Salvatore Feo, Patrizia Cancemi, Nadia Ninfa Albanese, Ida Pucci-Minafra, Elena Roz, Patrizia Cancemi

Risultato della ricerca: Articlepeer review

2 Citazioni (Scopus)

Abstract

Colon cancer is an aggressive tumor form with a poor prognosis. This study reports a comparative proteomic analysis performed by using two-dimensional differential in-gel electrophoresis (2D-DIGE) between 26 pooled colon cancer surgical tissues and adjacent non-tumoral tissues, to identify potential target proteins correlated with carcinogenesis. The DAVID functional classification tool revealed that most of the differentially regulated proteins, acting both intracellularly and extracellularly, concur across multiple cancer steps. The identified protein classes include proteins involved in cell proliferation, apoptosis, metabolic pathways, oxidative stress, cell motility, Ras signal transduction, and cytoskeleton. Interestingly, networks and pathways analysis showed that the identified proteins could be biologically inter-connected to the tumor-host microenvironment, including innate immune response, platelet and neutrophil degranulation, and hemostasis. Finally, transgelin (TAGL), here identified for the first time with four different protein species, collectively down-regulated in colon cancer tissues, emerged as a top-ranked biomarker for colorectal cancer (CRC). In conclusion, our findings revealed a different proteomic profiling in colon cancer tissues characterized by the deregulation of specific pathways involved in hallmarks of cancer. All of these proteins may represent promising novel colon cancer biomarkers and potential therapeutic targets, if validated in larger cohorts of patients.
Lingua originaleEnglish
pagine (da-a)1-17
Numero di pagine17
RivistaInternational Journal of Molecular Sciences
Volume21
Stato di pubblicazionePublished - 2020

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint Entra nei temi di ricerca di 'Proteomic Profiling of Colon Cancer Tissues: Discovery of New Candidate Biomarkers'. Insieme formano una fingerprint unica.

Cita questo