Proteomic patterns of cultured breast cancer cells and epithelial mammary cells.

Simona Fontana, Patrizia Cancemi, Salvatore Minafra

Risultato della ricerca: Articlepeer review

48 Citazioni (Scopus)

Abstract

Breast cancer is one of the leading causes of death from cancer among women in western countries. The different types of breast cancer are grouped into invasive and noninvasive forms. Among the invasive types, ductal infiltrating carcinoma (DIC) is the most common and aggressive form. Using an in vitro model consisting of a DIC-derived cell line (8701-BC) and a nontumoral mammary epithelial cell line (HB2), we used the proteomics approach to search for homology and differences in protein expression patterns between tumoral and nontumoral phenotypes. Within an analysis window comprising 1,750 discernible spots we have currently catalogued 140 protein spots of potential interest. Fifty-eight of them were identified by gel matching with reference maps, immunodetection, or N-terminal microsequencing and classified into four functional groups. Twelve proteins were found differentially expressed in two cell lines: four were uniquely present in the neoplastic cell proteome and eight in epithelial cells. In addition, 53 proteins displayed different relative expression levels between the two cell lines, that is, 44 were more elevated in cancer cells and 9 in HB2 cells. Among proteins with greater relative abundance in cancer cells we identified glycolytic enzymes (or their isoforms), which may indicate that the known metabolic dysregulation in cancer can reflect oncogenic-related defects of glycolytic gene expression.
Lingua originaleEnglish
Numero di pagine18
RivistaAnnals of the New York Academy of Sciences
Volume963
Stato di pubblicazionePublished - 2002

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1300.1300???
  • History and Philosophy of Science

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