Proteins participating to the post-transcriptional regulation of the mitochondrial cytochrome c oxidase subunit IV via elements located in the 3′UTR

Anna Maria Rinaldi, Elisa Ferruggia, Giuseppe Cannino

Risultato della ricerca: Articlepeer review

13 Citazioni (Scopus)

Abstract

In developing rat brain cytochrome c oxidase subunit IV (COXIV) expression is also regulated at posttranscriptionallevel and two 30UTR-COXIV RNA-binding factors have been identified.Here, we report the enrichment and identification of the factors from just born rat brains by affinitychromatography of biotinylated 30UTR-COXIV RNA–protein complexes on streptavidin-conjugated paramagneticparticles.We successfully isolated two main proteins of about 86 and 42 kDa, whose sequences were highlyattributable to Hsp90 and Actin. The purified proteins maintain RNA-binding ability and specificity forCOXIV messenger and, interacting with the 30UTR, then could negatively modulate mRNA translation.We also studied the content of Hsp90 and Actin during postnatal brain development and demonstratedthat in just born rat brain, when the COXIV protein appears at low level, Hsp90 was not phosphorylated.Vice versa in the adult tissue, when COXIV accumulates, Hsp90 appeared phosphorylated in serine thereforeit could be unable to bind COXIV messenger, suggesting that the phosphorylation event could provokethe loss of Hsp90 binding to mRNA.We hypothesize a new post-transcriptional mechanism regulating a messenger encoded by nucleargenome for a mitochondrial protein and that Hsp90 and Actin, could represent key players in COXIVtranslation.
Lingua originaleEnglish
Numero di pagine10
RivistaMitochondrion
Volume2009-08-22
Stato di pubblicazionePublished - 2009
Pubblicato esternamente

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