Protective effects of Aphanizomenon Flose-Aquae (AFA) extract on experimental colitis in rat

Risultato della ricerca: Other

Abstract

Inflammatory bowel diseases (IBD) are chronic immune disorders, whichnecessitate long-term dependence on powerful drugs. Recently, the use of natural product-based therapieshas emerged as a promising intervention for IBD. Aphanizomenon flose-aquae (AFA) is a unicellular bluegreenmicroalgae, traditionally used for its health-enhancing antioxidant and anti-inflammatory properties;we aimed to evaluate its protective effects on animal model of experimental colitis.Colitis was induced in rat by intracolonic instillation of 15 mg of 2,4-dinitrobenzene sulfonic acid (DNBS).Rats received AFA (100 mg/kg/d) by oral route, 4 days before and 6 days after colitis induction. The effectsof AFA on DNBS-induced colitis were evaluated by assessment of macroscopic, microscopic signs of colitisand by biochemical assay of the markers of inflammation.Obvious reduction of body weight, loose faeces/ diarrhoea, increase in colon weight/length ratio, colonicinflammation, mucosa injuries and increase in myeloperoxidase levels were observed in DNBS- groups.AFA pre-treatment improved body-weight, stool consistency, and colon shortening as well as attenuated theextent of colonic damage, likely protecting the mucosal epithelium. Colonic histopathological changes,cellular neutrophil infiltration and the subsequent increase in colonic myeloperoxidase levels induced byDNBS, were also reduced after AFA administration. AFA treatment was ineffective in control group.The present study, for the first time, shows the protective effects of AFA extract on DNBS-induced colitis inrats. Further studies could be addressed to evaluate if Blue-green alga AFA could be used as nutraceuticals inthe treatment of the IBD.
Lingua originaleEnglish
Pagine66-66
Numero di pagine1
Stato di pubblicazionePublished - 2018

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Aphanizomenon
Colitis
Sulfonic Acids
Dinitrobenzenes
Inflammatory Bowel Diseases
Peroxidase
Colon
Body Weight
Neutrophil Infiltration
Immune System Diseases
Cyanobacteria
Dietary Supplements
Biological Products
Feces
Diarrhea
Mucous Membrane
Anti-Inflammatory Agents
Epithelium
Animal Models
Antioxidants

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@conference{23bf1f8aaefa4983a54295c06b1e7d73,
title = "Protective effects of Aphanizomenon Flose-Aquae (AFA) extract on experimental colitis in rat",
abstract = "Inflammatory bowel diseases (IBD) are chronic immune disorders, whichnecessitate long-term dependence on powerful drugs. Recently, the use of natural product-based therapieshas emerged as a promising intervention for IBD. Aphanizomenon flose-aquae (AFA) is a unicellular bluegreenmicroalgae, traditionally used for its health-enhancing antioxidant and anti-inflammatory properties;we aimed to evaluate its protective effects on animal model of experimental colitis.Colitis was induced in rat by intracolonic instillation of 15 mg of 2,4-dinitrobenzene sulfonic acid (DNBS).Rats received AFA (100 mg/kg/d) by oral route, 4 days before and 6 days after colitis induction. The effectsof AFA on DNBS-induced colitis were evaluated by assessment of macroscopic, microscopic signs of colitisand by biochemical assay of the markers of inflammation.Obvious reduction of body weight, loose faeces/ diarrhoea, increase in colon weight/length ratio, colonicinflammation, mucosa injuries and increase in myeloperoxidase levels were observed in DNBS- groups.AFA pre-treatment improved body-weight, stool consistency, and colon shortening as well as attenuated theextent of colonic damage, likely protecting the mucosal epithelium. Colonic histopathological changes,cellular neutrophil infiltration and the subsequent increase in colonic myeloperoxidase levels induced byDNBS, were also reduced after AFA administration. AFA treatment was ineffective in control group.The present study, for the first time, shows the protective effects of AFA extract on DNBS-induced colitis inrats. Further studies could be addressed to evaluate if Blue-green alga AFA could be used as nutraceuticals inthe treatment of the IBD.",
author = "Antonella Amato and Zizzo, {Maria Grazia} and Serio, {Rosa Maria} and Caldara, {Gaetano Felice} and Annalisa Bellanca",
year = "2018",
language = "English",
pages = "66--66",

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TY - CONF

T1 - Protective effects of Aphanizomenon Flose-Aquae (AFA) extract on experimental colitis in rat

AU - Amato, Antonella

AU - Zizzo, Maria Grazia

AU - Serio, Rosa Maria

AU - Caldara, Gaetano Felice

AU - Bellanca, Annalisa

PY - 2018

Y1 - 2018

N2 - Inflammatory bowel diseases (IBD) are chronic immune disorders, whichnecessitate long-term dependence on powerful drugs. Recently, the use of natural product-based therapieshas emerged as a promising intervention for IBD. Aphanizomenon flose-aquae (AFA) is a unicellular bluegreenmicroalgae, traditionally used for its health-enhancing antioxidant and anti-inflammatory properties;we aimed to evaluate its protective effects on animal model of experimental colitis.Colitis was induced in rat by intracolonic instillation of 15 mg of 2,4-dinitrobenzene sulfonic acid (DNBS).Rats received AFA (100 mg/kg/d) by oral route, 4 days before and 6 days after colitis induction. The effectsof AFA on DNBS-induced colitis were evaluated by assessment of macroscopic, microscopic signs of colitisand by biochemical assay of the markers of inflammation.Obvious reduction of body weight, loose faeces/ diarrhoea, increase in colon weight/length ratio, colonicinflammation, mucosa injuries and increase in myeloperoxidase levels were observed in DNBS- groups.AFA pre-treatment improved body-weight, stool consistency, and colon shortening as well as attenuated theextent of colonic damage, likely protecting the mucosal epithelium. Colonic histopathological changes,cellular neutrophil infiltration and the subsequent increase in colonic myeloperoxidase levels induced byDNBS, were also reduced after AFA administration. AFA treatment was ineffective in control group.The present study, for the first time, shows the protective effects of AFA extract on DNBS-induced colitis inrats. Further studies could be addressed to evaluate if Blue-green alga AFA could be used as nutraceuticals inthe treatment of the IBD.

AB - Inflammatory bowel diseases (IBD) are chronic immune disorders, whichnecessitate long-term dependence on powerful drugs. Recently, the use of natural product-based therapieshas emerged as a promising intervention for IBD. Aphanizomenon flose-aquae (AFA) is a unicellular bluegreenmicroalgae, traditionally used for its health-enhancing antioxidant and anti-inflammatory properties;we aimed to evaluate its protective effects on animal model of experimental colitis.Colitis was induced in rat by intracolonic instillation of 15 mg of 2,4-dinitrobenzene sulfonic acid (DNBS).Rats received AFA (100 mg/kg/d) by oral route, 4 days before and 6 days after colitis induction. The effectsof AFA on DNBS-induced colitis were evaluated by assessment of macroscopic, microscopic signs of colitisand by biochemical assay of the markers of inflammation.Obvious reduction of body weight, loose faeces/ diarrhoea, increase in colon weight/length ratio, colonicinflammation, mucosa injuries and increase in myeloperoxidase levels were observed in DNBS- groups.AFA pre-treatment improved body-weight, stool consistency, and colon shortening as well as attenuated theextent of colonic damage, likely protecting the mucosal epithelium. Colonic histopathological changes,cellular neutrophil infiltration and the subsequent increase in colonic myeloperoxidase levels induced byDNBS, were also reduced after AFA administration. AFA treatment was ineffective in control group.The present study, for the first time, shows the protective effects of AFA extract on DNBS-induced colitis inrats. Further studies could be addressed to evaluate if Blue-green alga AFA could be used as nutraceuticals inthe treatment of the IBD.

UR - http://hdl.handle.net/10447/294051

M3 - Other

SP - 66

EP - 66

ER -