Production of an egg yolk antibody against Parietaria judaica 2 allergen.

Giacomo De Leo, Simona Fontana, Riccardo Alessandro, Simona Taverna, Marilisa Barranca, Giovanni Cassata, Giovanni Duro, Michel Becchi, Alessia Gallo, Simona Principe

Risultato della ricerca: Articlepeer review

9 Citazioni (Scopus)


Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combination of molecular biology, 2-dimensional PAGE, and use of nonmammalian antibodies represents a powerful tool for reliable identification of allergens.
Lingua originaleEnglish
pagine (da-a)1773-1778
Numero di pagine6
RivistaPoultry Science
Stato di pubblicazionePublished - 2009

All Science Journal Classification (ASJC) codes

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