Probing in cell protein structural changes with time-resolved X-ray scattering

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Abstract

Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.
Lingua originaleEnglish
pagine (da-a)6434-6437
Numero di pagine4
RivistaSoft Matter
Volume8
Stato di pubblicazionePublished - 2012

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Molecular biology
Photolysis
X ray scattering
Hemoglobins
Blood
Cells
proteins
molecular biology
Lasers
hemoglobin
erythrocytes
cells
scattering
flash
photolysis
Proteins
x rays
lasers

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Condensed Matter Physics

Cita questo

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title = "Probing in cell protein structural changes with time-resolved X-ray scattering",
abstract = "Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.",
author = "Antonio Cupane and Alessandro Spilotros and Matteo Levantino and Giorgio Schiro' and Marco Cammarata and Michael Wulff",
year = "2012",
language = "English",
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journal = "Soft Matter",
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publisher = "Royal Society of Chemistry",

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T1 - Probing in cell protein structural changes with time-resolved X-ray scattering

AU - Cupane, Antonio

AU - Spilotros, Alessandro

AU - Levantino, Matteo

AU - Schiro', Giorgio

AU - Cammarata, Marco

AU - Wulff, Michael

PY - 2012

Y1 - 2012

N2 - Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.

AB - Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.

UR - http://hdl.handle.net/10447/63231

UR - http://pubs.rsc.org/en/content/articlelanding/2012/sm/c2sm25676b

M3 - Article

VL - 8

SP - 6434

EP - 6437

JO - Soft Matter

JF - Soft Matter

SN - 1744-683X

ER -