Prevalenza di infezione da patogeni a trasmissione ematica (HIV,HBV,HCV)in tossicodipendenti afferenti ai SERT dell'area palermitana

Nino Romano, Fabio Tramuto, Francesco Vitale, Georgina Mbisa, Amy Hutchinson, James J. Goedert, Elizabeth E. Brown, Carmela Lauria, Scott Durum, Stephen J. Chanock, Diego Serraino, Denise Whitby, Massimo Giuliani, Angelo Messina, Vickie Marshall, M. Daniele Fallin

Risultato della ricerca: Articlepeer review

26 Citazioni (Scopus)

Abstract

Background. Kaposi sarcoma (KS) is primarily caused by human herpesvirus (HHV)-8 infection, and the risk is increased with high HHV-8 lytic or latent antibody titers or the detection of HHV-8 DNA in peripheral blood mononuclear cells (PBMCs). Host genes important for control of HHV-8 infection are not well characterized. Methods. In 172 HHV-8 latent nuclear antigen (LANA)-seropositive adults in Italy without KS, we examined correlations of common variants in host immune genes with the detection of HHV-8 DNA in PBMCs and with high lytic and latent antibody titers. Twenty-eight single-nucleotide polymorphisms in 14 genes were analyzed. We detected HHV-8 DNA in PBMCs with real-time amplification of the K6 gene, anti-K8.1 (lytic) titers with enzyme-linked immunosorbent assay, and anti-LANA (latent) titers with immunofluorescence. Results. Detection of HHV-8 DNA in PBMCs was not significantly related to any variant examined. In contrast, a 3-locus haplotype of IL4, which contains the -1098G allele (rs2243248), was overrepresented among subjects with high lytic titers (odds ratio [OR], 2.8 [95% confidence interval {CI}, 1.1-6.7]), compared with those with low titers, as was the functional promoter variant of IL6, C-236C (rs1800795) (OR, 3.7 [95% CI, 1.1-12.8]). Compared with subjects with low HHV-8 latent antibody titers, analysis of inferred haplotypes for IL12A revealed an overrepresentation of -798T/277A in subjects with high HHV-8 latent antibody titers (OR, 2.4 [95% CI, 1.1-5.2]). Conclusions. Our observations are the first to provide preliminary evidence suggesting that common variants in key host immune genes could influence the control of HHV-8 infection.
Lingua originaleUndefined/Unknown
pagine (da-a)218-218
RivistaJournal of Preventive Medicine and Hygiene
Volume45/4
Stato di pubblicazionePublished - 2004

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

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