Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury.

Maria Rosaria Bonsignore, Laura Chimenti, Ramon Farre, José Ramírez, Tomás Luque, Daniel Navajas

Risultato della ricerca: Article

22 Citazioni (Scopus)

Abstract

ckground information: Bone marrow-derived mesenchymal stem cells (MSCs) reduce acute lung injury in animals challenged by bleomycin or bacterial lipopolysaccaride. It is not known, however, whether MSCs protect from ventilator- induced lung injury (VILI). Question of the study: Whether MSCs have a potential role in preventing or modulating VILI in healthy rats subjected to high-volume ventilation. Materials and methods: 24 Sprague-Dawley rats (250-300 g) were subjected to high- volume mechanical ventilation (25 ml/kg). MSCs (5x106) were intravenously or intratracheally administered (N=8 each) 30 min before starting over-ventilation and 8 rats were MSC-untreated. Spontaneously breathing anesthetised rats (N=8) served as controls. After 3 h of over-ventilation/control the animals were sacrificed and lung tissue and bronchoalveolar lavage fluid (BALF) were sampled for further analysis. Results: When compared with controls, MSC-untreated over-ventilated rats exhibited typical VILI features. Lung edema histological lung injury index, concentrations of total protein, interleukin-1β, macrophage inflammatory protein-2 and number of neutrophils in BALF and vascular cell adhesion protein-1 in lung tissue significantly increased in overventilated rats. All these indices of VILI moved significantly towards normalization in the rats treated with MSCs, whether intravenously or intratracheally. Answer to question: Both local and systemic pre-treatment with MSCs reduced VILI in a rat model.
Lingua originaleEnglish
pagine (da-a)939-948
Numero di pagine10
RivistaEuropean Respiratory Journal
Volume40
Stato di pubblicazionePublished - 2012

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Ventilator-Induced Lung Injury
Mesenchymal Stromal Cells
Bronchoalveolar Lavage Fluid
Ventilation
Therapeutics
Chemokine CXCL2
Lung
Acute Lung Injury
Bleomycin
Lung Injury
Interleukin-1beta
Artificial Respiration
Cell Adhesion
Blood Vessels
Sprague Dawley Rats
Edema
Respiration
Proteins
Neutrophils
Bone Marrow

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cita questo

Bonsignore, M. R., Chimenti, L., Farre, R., Ramírez, J., Luque, T., & Navajas, D. (2012). Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury. European Respiratory Journal, 40, 939-948.

Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury. / Bonsignore, Maria Rosaria; Chimenti, Laura; Farre, Ramon; Ramírez, José; Luque, Tomás; Navajas, Daniel.

In: European Respiratory Journal, Vol. 40, 2012, pag. 939-948.

Risultato della ricerca: Article

Bonsignore, MR, Chimenti, L, Farre, R, Ramírez, J, Luque, T & Navajas, D 2012, 'Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury.', European Respiratory Journal, vol. 40, pagg. 939-948.
Bonsignore, Maria Rosaria ; Chimenti, Laura ; Farre, Ramon ; Ramírez, José ; Luque, Tomás ; Navajas, Daniel. / Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury. In: European Respiratory Journal. 2012 ; Vol. 40. pagg. 939-948.
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AU - Luque, Tomás

AU - Navajas, Daniel

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N2 - ckground information: Bone marrow-derived mesenchymal stem cells (MSCs) reduce acute lung injury in animals challenged by bleomycin or bacterial lipopolysaccaride. It is not known, however, whether MSCs protect from ventilator- induced lung injury (VILI). Question of the study: Whether MSCs have a potential role in preventing or modulating VILI in healthy rats subjected to high-volume ventilation. Materials and methods: 24 Sprague-Dawley rats (250-300 g) were subjected to high- volume mechanical ventilation (25 ml/kg). MSCs (5x106) were intravenously or intratracheally administered (N=8 each) 30 min before starting over-ventilation and 8 rats were MSC-untreated. Spontaneously breathing anesthetised rats (N=8) served as controls. After 3 h of over-ventilation/control the animals were sacrificed and lung tissue and bronchoalveolar lavage fluid (BALF) were sampled for further analysis. Results: When compared with controls, MSC-untreated over-ventilated rats exhibited typical VILI features. Lung edema histological lung injury index, concentrations of total protein, interleukin-1β, macrophage inflammatory protein-2 and number of neutrophils in BALF and vascular cell adhesion protein-1 in lung tissue significantly increased in overventilated rats. All these indices of VILI moved significantly towards normalization in the rats treated with MSCs, whether intravenously or intratracheally. Answer to question: Both local and systemic pre-treatment with MSCs reduced VILI in a rat model.

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