PPAR alpha gene variants as predicted performance-enhancing polymorphisms in professional Italian soccer players

Risultato della ricerca: Article

Abstract

Background: The PPARα gene encodes the peroxisome proliferator-activator receptor alpha, acentral regulator of expression of other genes involved in fatty acid metabolism. The purpose ofthis study was to determine the prevalence of G allele of the PPARα intron 7 G/C polymorphism(rs4253778) in professional Italian soccer players.Methods: Sixty professional soccer players and 30 sedentary volunteers were enrolled in thestudy. Samples of venous blood were obtained at rest, in the morning, by conventional clinicalprocedures; blood serum was collected and total cholesterol, high-density lipoprotein cholesterol,low-density lipoprotein cholesterol, and triglycerides were measured. An aliquot of anticoagulanttreatedblood was used to prepare genomic DNA from whole blood. The G/C polymorphic sitein PPARα intron 7 was scanned by using the PCR-RFLP (polymerase chain reaction restrictionfragment length polymorphism) protocol with TaqI enzyme.Results: We found variations in genotype distribution of PPARα polymorphism betweenprofessional soccer players and sedentary volunteers. Particularly, G alleles and the GG genotypewere significantly more frequent in soccer players compared with healthy controls (64% versus48%). No significant correlations were found between lipid profile and genotype background.Conclusion: Previous results demonstrated an association of intron 7 G allele as well as theGG genotype in endurance athletes. Our result suggests that this is the case also in professionalsoccer players.
Lingua originaleEnglish
pagine (da-a)273-278
Numero di pagine6
RivistaOPEN ACCESS JOURNAL OF SPORTS MEDICINE
Volume5
Stato di pubblicazionePublished - 2014

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PPAR alpha
Peroxisome Proliferator-Activated Receptors
Soccer
Introns
Alleles
Genotype
Genes
Volunteers
Peroxisome Proliferators
Athletes
LDL Cholesterol
HDL Cholesterol
Fatty Acids
Cholesterol
Lipids
Gene Expression
Polymerase Chain Reaction
DNA
Enzymes
Serum

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@article{8a1973f83d4e4661ba95ee7f89d3e24b,
title = "PPAR alpha gene variants as predicted performance-enhancing polymorphisms in professional Italian soccer players",
abstract = "Background: The PPARα gene encodes the peroxisome proliferator-activator receptor alpha, acentral regulator of expression of other genes involved in fatty acid metabolism. The purpose ofthis study was to determine the prevalence of G allele of the PPARα intron 7 G/C polymorphism(rs4253778) in professional Italian soccer players.Methods: Sixty professional soccer players and 30 sedentary volunteers were enrolled in thestudy. Samples of venous blood were obtained at rest, in the morning, by conventional clinicalprocedures; blood serum was collected and total cholesterol, high-density lipoprotein cholesterol,low-density lipoprotein cholesterol, and triglycerides were measured. An aliquot of anticoagulanttreatedblood was used to prepare genomic DNA from whole blood. The G/C polymorphic sitein PPARα intron 7 was scanned by using the PCR-RFLP (polymerase chain reaction restrictionfragment length polymorphism) protocol with TaqI enzyme.Results: We found variations in genotype distribution of PPARα polymorphism betweenprofessional soccer players and sedentary volunteers. Particularly, G alleles and the GG genotypewere significantly more frequent in soccer players compared with healthy controls (64{\%} versus48{\%}). No significant correlations were found between lipid profile and genotype background.Conclusion: Previous results demonstrated an association of intron 7 G allele as well as theGG genotype in endurance athletes. Our result suggests that this is the case also in professionalsoccer players.",
author = "Marcello Traina and Antonio Palma and Patrizia Saladino and Antonino Bianco and Gabriella Schiera and Patrizia Proia",
year = "2014",
language = "English",
volume = "5",
pages = "273--278",
journal = "OPEN ACCESS JOURNAL OF SPORTS MEDICINE",
issn = "1179-1543",

}

TY - JOUR

T1 - PPAR alpha gene variants as predicted performance-enhancing polymorphisms in professional Italian soccer players

AU - Traina, Marcello

AU - Palma, Antonio

AU - Saladino, Patrizia

AU - Bianco, Antonino

AU - Schiera, Gabriella

AU - Proia, Patrizia

PY - 2014

Y1 - 2014

N2 - Background: The PPARα gene encodes the peroxisome proliferator-activator receptor alpha, acentral regulator of expression of other genes involved in fatty acid metabolism. The purpose ofthis study was to determine the prevalence of G allele of the PPARα intron 7 G/C polymorphism(rs4253778) in professional Italian soccer players.Methods: Sixty professional soccer players and 30 sedentary volunteers were enrolled in thestudy. Samples of venous blood were obtained at rest, in the morning, by conventional clinicalprocedures; blood serum was collected and total cholesterol, high-density lipoprotein cholesterol,low-density lipoprotein cholesterol, and triglycerides were measured. An aliquot of anticoagulanttreatedblood was used to prepare genomic DNA from whole blood. The G/C polymorphic sitein PPARα intron 7 was scanned by using the PCR-RFLP (polymerase chain reaction restrictionfragment length polymorphism) protocol with TaqI enzyme.Results: We found variations in genotype distribution of PPARα polymorphism betweenprofessional soccer players and sedentary volunteers. Particularly, G alleles and the GG genotypewere significantly more frequent in soccer players compared with healthy controls (64% versus48%). No significant correlations were found between lipid profile and genotype background.Conclusion: Previous results demonstrated an association of intron 7 G allele as well as theGG genotype in endurance athletes. Our result suggests that this is the case also in professionalsoccer players.

AB - Background: The PPARα gene encodes the peroxisome proliferator-activator receptor alpha, acentral regulator of expression of other genes involved in fatty acid metabolism. The purpose ofthis study was to determine the prevalence of G allele of the PPARα intron 7 G/C polymorphism(rs4253778) in professional Italian soccer players.Methods: Sixty professional soccer players and 30 sedentary volunteers were enrolled in thestudy. Samples of venous blood were obtained at rest, in the morning, by conventional clinicalprocedures; blood serum was collected and total cholesterol, high-density lipoprotein cholesterol,low-density lipoprotein cholesterol, and triglycerides were measured. An aliquot of anticoagulanttreatedblood was used to prepare genomic DNA from whole blood. The G/C polymorphic sitein PPARα intron 7 was scanned by using the PCR-RFLP (polymerase chain reaction restrictionfragment length polymorphism) protocol with TaqI enzyme.Results: We found variations in genotype distribution of PPARα polymorphism betweenprofessional soccer players and sedentary volunteers. Particularly, G alleles and the GG genotypewere significantly more frequent in soccer players compared with healthy controls (64% versus48%). No significant correlations were found between lipid profile and genotype background.Conclusion: Previous results demonstrated an association of intron 7 G allele as well as theGG genotype in endurance athletes. Our result suggests that this is the case also in professionalsoccer players.

UR - http://hdl.handle.net/10447/97866

M3 - Article

VL - 5

SP - 273

EP - 278

JO - OPEN ACCESS JOURNAL OF SPORTS MEDICINE

JF - OPEN ACCESS JOURNAL OF SPORTS MEDICINE

SN - 1179-1543

ER -