Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 273-278 |
| Numero di pagine | 6 |
| Rivista | OPEN ACCESS JOURNAL OF SPORTS MEDICINE |
| Volume | 5 |
| Stato di pubblicazione | Published - 2014 |
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PPAR alpha gene variants as predicted performance-enhancing polymorphisms in professional Italian soccer players. / Traina, Marcello; Palma, Antonio; Saladino, Patrizia; Bianco, Antonino; Schiera, Gabriella; Proia, Patrizia.
In: OPEN ACCESS JOURNAL OF SPORTS MEDICINE, Vol. 5, 2014, pag. 273-278.Risultato della ricerca: Article
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TY - JOUR
T1 - PPAR alpha gene variants as predicted performance-enhancing polymorphisms in professional Italian soccer players
AU - Traina, Marcello
AU - Palma, Antonio
AU - Saladino, Patrizia
AU - Bianco, Antonino
AU - Schiera, Gabriella
AU - Proia, Patrizia
PY - 2014
Y1 - 2014
N2 - Background: The PPARα gene encodes the peroxisome proliferator-activator receptor alpha, acentral regulator of expression of other genes involved in fatty acid metabolism. The purpose ofthis study was to determine the prevalence of G allele of the PPARα intron 7 G/C polymorphism(rs4253778) in professional Italian soccer players.Methods: Sixty professional soccer players and 30 sedentary volunteers were enrolled in thestudy. Samples of venous blood were obtained at rest, in the morning, by conventional clinicalprocedures; blood serum was collected and total cholesterol, high-density lipoprotein cholesterol,low-density lipoprotein cholesterol, and triglycerides were measured. An aliquot of anticoagulanttreatedblood was used to prepare genomic DNA from whole blood. The G/C polymorphic sitein PPARα intron 7 was scanned by using the PCR-RFLP (polymerase chain reaction restrictionfragment length polymorphism) protocol with TaqI enzyme.Results: We found variations in genotype distribution of PPARα polymorphism betweenprofessional soccer players and sedentary volunteers. Particularly, G alleles and the GG genotypewere significantly more frequent in soccer players compared with healthy controls (64% versus48%). No significant correlations were found between lipid profile and genotype background.Conclusion: Previous results demonstrated an association of intron 7 G allele as well as theGG genotype in endurance athletes. Our result suggests that this is the case also in professionalsoccer players.
AB - Background: The PPARα gene encodes the peroxisome proliferator-activator receptor alpha, acentral regulator of expression of other genes involved in fatty acid metabolism. The purpose ofthis study was to determine the prevalence of G allele of the PPARα intron 7 G/C polymorphism(rs4253778) in professional Italian soccer players.Methods: Sixty professional soccer players and 30 sedentary volunteers were enrolled in thestudy. Samples of venous blood were obtained at rest, in the morning, by conventional clinicalprocedures; blood serum was collected and total cholesterol, high-density lipoprotein cholesterol,low-density lipoprotein cholesterol, and triglycerides were measured. An aliquot of anticoagulanttreatedblood was used to prepare genomic DNA from whole blood. The G/C polymorphic sitein PPARα intron 7 was scanned by using the PCR-RFLP (polymerase chain reaction restrictionfragment length polymorphism) protocol with TaqI enzyme.Results: We found variations in genotype distribution of PPARα polymorphism betweenprofessional soccer players and sedentary volunteers. Particularly, G alleles and the GG genotypewere significantly more frequent in soccer players compared with healthy controls (64% versus48%). No significant correlations were found between lipid profile and genotype background.Conclusion: Previous results demonstrated an association of intron 7 G allele as well as theGG genotype in endurance athletes. Our result suggests that this is the case also in professionalsoccer players.
UR - http://hdl.handle.net/10447/97866
M3 - Article
VL - 5
SP - 273
EP - 278
JO - OPEN ACCESS JOURNAL OF SPORTS MEDICINE
JF - OPEN ACCESS JOURNAL OF SPORTS MEDICINE
SN - 1179-1543
ER -