Abstract

PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα codingfor peroxisome proliferator activator receptor alpha, a central regulator of expressionof genes involved in fatty acid metabolism. The aim of our research was to study therole of polymorphism of PPARα gene in performance enhancing in Italian soccerplayers.METHODS: Sixty professional soccer players and thirty sedentary volunteers wereenrolled in the study. Samples of venous blood were obtained at rest in the morning byconventional clinical procedures. Serum was collected and lipid profile was measuredby using a commercial kit. An aliquote of anticoagulant-treated blood was used toprepare genomic DNA from mononuclear cells. The polymorphic site in PPARα intron 7was scanned by using PCR-RFLP protocol with Taq I enzyme.RESULTS: We found variations among genotype distribution of PPARα in professionalsoccer players versus sedentary volunteers. To enphatyzed this correlation, we alsoinvestigated lipidic profile variation.CONCLUSIONS: Our results evidenced an high expression of G allele as well as GGgenotype in accordance with other studies that associate this genotype withendurance performance. So PPARα G allele may be considered an endurance relatesallele. We can explain this one because PPARα is an important factor that regulate thebalance between fatty acid and glucose metabolism. Moreover, we analyzed thecorrelation between G allele distribution and athletes lipid profile but we didn’t findany correlation.
Lingua originaleEnglish
Numero di pagine0
Stato di pubblicazionePublished - 2012

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Peroxisome Proliferator-Activated Receptors
Soccer
Genes
Alleles
Volunteers
Fatty Acids
Genotype
Peroxisome Proliferators
Lipids
Regulator Genes
Athletes
Restriction Fragment Length Polymorphisms
Anticoagulants
Introns
Phenotype
Glucose
Polymerase Chain Reaction
DNA
Health
Enzymes

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@conference{eab77f4ef53445b29af46c707d5b23af,
title = "PPARα gene variation and phisycal performance in italian soccer players",
abstract = "PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα codingfor peroxisome proliferator activator receptor alpha, a central regulator of expressionof genes involved in fatty acid metabolism. The aim of our research was to study therole of polymorphism of PPARα gene in performance enhancing in Italian soccerplayers.METHODS: Sixty professional soccer players and thirty sedentary volunteers wereenrolled in the study. Samples of venous blood were obtained at rest in the morning byconventional clinical procedures. Serum was collected and lipid profile was measuredby using a commercial kit. An aliquote of anticoagulant-treated blood was used toprepare genomic DNA from mononuclear cells. The polymorphic site in PPARα intron 7was scanned by using PCR-RFLP protocol with Taq I enzyme.RESULTS: We found variations among genotype distribution of PPARα in professionalsoccer players versus sedentary volunteers. To enphatyzed this correlation, we alsoinvestigated lipidic profile variation.CONCLUSIONS: Our results evidenced an high expression of G allele as well as GGgenotype in accordance with other studies that associate this genotype withendurance performance. So PPARα G allele may be considered an endurance relatesallele. We can explain this one because PPARα is an important factor that regulate thebalance between fatty acid and glucose metabolism. Moreover, we analyzed thecorrelation between G allele distribution and athletes lipid profile but we didn’t findany correlation.",
keywords = "PPARα",
author = "Marcello Traina and Antonino Bianco and Patrizia Proia and Patrizia Saladino and Gabriella Schiera and Antonio Palma",
year = "2012",
language = "English",

}

TY - CONF

T1 - PPARα gene variation and phisycal performance in italian soccer players

AU - Traina, Marcello

AU - Bianco, Antonino

AU - Proia, Patrizia

AU - Saladino, Patrizia

AU - Schiera, Gabriella

AU - Palma, Antonio

PY - 2012

Y1 - 2012

N2 - PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα codingfor peroxisome proliferator activator receptor alpha, a central regulator of expressionof genes involved in fatty acid metabolism. The aim of our research was to study therole of polymorphism of PPARα gene in performance enhancing in Italian soccerplayers.METHODS: Sixty professional soccer players and thirty sedentary volunteers wereenrolled in the study. Samples of venous blood were obtained at rest in the morning byconventional clinical procedures. Serum was collected and lipid profile was measuredby using a commercial kit. An aliquote of anticoagulant-treated blood was used toprepare genomic DNA from mononuclear cells. The polymorphic site in PPARα intron 7was scanned by using PCR-RFLP protocol with Taq I enzyme.RESULTS: We found variations among genotype distribution of PPARα in professionalsoccer players versus sedentary volunteers. To enphatyzed this correlation, we alsoinvestigated lipidic profile variation.CONCLUSIONS: Our results evidenced an high expression of G allele as well as GGgenotype in accordance with other studies that associate this genotype withendurance performance. So PPARα G allele may be considered an endurance relatesallele. We can explain this one because PPARα is an important factor that regulate thebalance between fatty acid and glucose metabolism. Moreover, we analyzed thecorrelation between G allele distribution and athletes lipid profile but we didn’t findany correlation.

AB - PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα codingfor peroxisome proliferator activator receptor alpha, a central regulator of expressionof genes involved in fatty acid metabolism. The aim of our research was to study therole of polymorphism of PPARα gene in performance enhancing in Italian soccerplayers.METHODS: Sixty professional soccer players and thirty sedentary volunteers wereenrolled in the study. Samples of venous blood were obtained at rest in the morning byconventional clinical procedures. Serum was collected and lipid profile was measuredby using a commercial kit. An aliquote of anticoagulant-treated blood was used toprepare genomic DNA from mononuclear cells. The polymorphic site in PPARα intron 7was scanned by using PCR-RFLP protocol with Taq I enzyme.RESULTS: We found variations among genotype distribution of PPARα in professionalsoccer players versus sedentary volunteers. To enphatyzed this correlation, we alsoinvestigated lipidic profile variation.CONCLUSIONS: Our results evidenced an high expression of G allele as well as GGgenotype in accordance with other studies that associate this genotype withendurance performance. So PPARα G allele may be considered an endurance relatesallele. We can explain this one because PPARα is an important factor that regulate thebalance between fatty acid and glucose metabolism. Moreover, we analyzed thecorrelation between G allele distribution and athletes lipid profile but we didn’t findany correlation.

KW - PPARα

UR - http://hdl.handle.net/10447/68724

M3 - Other

ER -