PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα codingfor peroxisome proliferator activator receptor alpha, a central regulator of expressionof genes involved in fatty acid metabolism. The aim of our research was to study therole of polymorphism of PPARα gene in performance enhancing in Italian soccerplayers.METHODS: Sixty professional soccer players and thirty sedentary volunteers wereenrolled in the study. Samples of venous blood were obtained at rest in the morning byconventional clinical procedures. Serum was collected and lipid profile was measuredby using a commercial kit. An aliquote of anticoagulant-treated blood was used toprepare genomic DNA from mononuclear cells. The polymorphic site in PPARα intron 7was scanned by using PCR-RFLP protocol with Taq I enzyme.RESULTS: We found variations among genotype distribution of PPARα in professionalsoccer players versus sedentary volunteers. To enphatyzed this correlation, we alsoinvestigated lipidic profile variation.CONCLUSIONS: Our results evidenced an high expression of G allele as well as GGgenotype in accordance with other studies that associate this genotype withendurance performance. So PPARα G allele may be considered an endurance relatesallele. We can explain this one because PPARα is an important factor that regulate thebalance between fatty acid and glucose metabolism. Moreover, we analyzed thecorrelation between G allele distribution and athletes lipid profile but we didn’t findany correlation.
|Numero di pagine||0|
|Stato di pubblicazione||Published - 2012|