PPARα gene variation and phisycal performance in italian soccer players

Contrò, V

Risultato della ricerca: Paper

Abstract

PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα coding for peroxisome proliferator activator receptor alpha, a central regulator of expression of genes involved in fatty acid metabolism. The aim of our research was to study the role of polymorphism of PPARα gene in performance enhancing in Italian soccer players. METHODS: Sixty professional soccer players and thirty sedentary volunteers were enrolled in the study. Samples of venous blood were obtained at rest in the morning by conventional clinical procedures. Serum was collected and lipid profile was measured by using a commercial kit. An aliquote of anticoagulant-treated blood was used to prepare genomic DNA from mononuclear cells. The polymorphic site in PPARα intron 7 was scanned by using PCR-RFLP protocol with Taq I enzyme. RESULTS: We found variations among genotype distribution of PPARα in professional soccer players versus sedentary volunteers. To enphatyzed this correlation, we also investigated lipidic profile variation. CONCLUSIONS: Our results evidenced an high expression of G allele as well as GG genotype in accordance with other studies that associate this genotype with endurance performance. So PPARα G allele may be considered an endurance relates allele. We can explain this one because PPARα is an important factor that regulate the balance between fatty acid and glucose metabolism. Moreover, we analyzed the correlation between G allele distribution and athletes lipid profile but we didn’t find any correlation.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2012

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Soccer
Alleles
Volunteers
Fatty Acids
Genotype
Genes
Peroxisome Proliferators
Lipids
Regulator Genes
Athletes
Restriction Fragment Length Polymorphisms
Anticoagulants
Introns
Phenotype
Glucose
Polymerase Chain Reaction
DNA
Health
Enzymes
Serum

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PPARα gene variation and phisycal performance in italian soccer players. / Contrò, V.

2012.

Risultato della ricerca: Paper

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title = "PPARα gene variation and phisycal performance in italian soccer players",
abstract = "PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα coding for peroxisome proliferator activator receptor alpha, a central regulator of expression of genes involved in fatty acid metabolism. The aim of our research was to study the role of polymorphism of PPARα gene in performance enhancing in Italian soccer players. METHODS: Sixty professional soccer players and thirty sedentary volunteers were enrolled in the study. Samples of venous blood were obtained at rest in the morning by conventional clinical procedures. Serum was collected and lipid profile was measured by using a commercial kit. An aliquote of anticoagulant-treated blood was used to prepare genomic DNA from mononuclear cells. The polymorphic site in PPARα intron 7 was scanned by using PCR-RFLP protocol with Taq I enzyme. RESULTS: We found variations among genotype distribution of PPARα in professional soccer players versus sedentary volunteers. To enphatyzed this correlation, we also investigated lipidic profile variation. CONCLUSIONS: Our results evidenced an high expression of G allele as well as GG genotype in accordance with other studies that associate this genotype with endurance performance. So PPARα G allele may be considered an endurance relates allele. We can explain this one because PPARα is an important factor that regulate the balance between fatty acid and glucose metabolism. Moreover, we analyzed the correlation between G allele distribution and athletes lipid profile but we didn’t find any correlation.",
keywords = "PPARα",
author = "{Contr{\`o}, V} and Marcello Traina and Antonio Palma and Gabriella Schiera and Patrizia Proia and Antonino Bianco and Patrizia Saladino",
year = "2012",
language = "English",

}

TY - CONF

T1 - PPARα gene variation and phisycal performance in italian soccer players

AU - Contrò, V

AU - Traina, Marcello

AU - Palma, Antonio

AU - Schiera, Gabriella

AU - Proia, Patrizia

AU - Bianco, Antonino

AU - Saladino, Patrizia

PY - 2012

Y1 - 2012

N2 - PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα coding for peroxisome proliferator activator receptor alpha, a central regulator of expression of genes involved in fatty acid metabolism. The aim of our research was to study the role of polymorphism of PPARα gene in performance enhancing in Italian soccer players. METHODS: Sixty professional soccer players and thirty sedentary volunteers were enrolled in the study. Samples of venous blood were obtained at rest in the morning by conventional clinical procedures. Serum was collected and lipid profile was measured by using a commercial kit. An aliquote of anticoagulant-treated blood was used to prepare genomic DNA from mononuclear cells. The polymorphic site in PPARα intron 7 was scanned by using PCR-RFLP protocol with Taq I enzyme. RESULTS: We found variations among genotype distribution of PPARα in professional soccer players versus sedentary volunteers. To enphatyzed this correlation, we also investigated lipidic profile variation. CONCLUSIONS: Our results evidenced an high expression of G allele as well as GG genotype in accordance with other studies that associate this genotype with endurance performance. So PPARα G allele may be considered an endurance relates allele. We can explain this one because PPARα is an important factor that regulate the balance between fatty acid and glucose metabolism. Moreover, we analyzed the correlation between G allele distribution and athletes lipid profile but we didn’t find any correlation.

AB - PURPOSE: One of the genes of the health-related fitness phenotype is a PPARα coding for peroxisome proliferator activator receptor alpha, a central regulator of expression of genes involved in fatty acid metabolism. The aim of our research was to study the role of polymorphism of PPARα gene in performance enhancing in Italian soccer players. METHODS: Sixty professional soccer players and thirty sedentary volunteers were enrolled in the study. Samples of venous blood were obtained at rest in the morning by conventional clinical procedures. Serum was collected and lipid profile was measured by using a commercial kit. An aliquote of anticoagulant-treated blood was used to prepare genomic DNA from mononuclear cells. The polymorphic site in PPARα intron 7 was scanned by using PCR-RFLP protocol with Taq I enzyme. RESULTS: We found variations among genotype distribution of PPARα in professional soccer players versus sedentary volunteers. To enphatyzed this correlation, we also investigated lipidic profile variation. CONCLUSIONS: Our results evidenced an high expression of G allele as well as GG genotype in accordance with other studies that associate this genotype with endurance performance. So PPARα G allele may be considered an endurance relates allele. We can explain this one because PPARα is an important factor that regulate the balance between fatty acid and glucose metabolism. Moreover, we analyzed the correlation between G allele distribution and athletes lipid profile but we didn’t find any correlation.

KW - PPARα

UR - http://hdl.handle.net/10447/68724

M3 - Paper

ER -