Potential use of new diphenylurea derivatives in micropropagation of Capparis spinosa L.

Francesco Sottile, Maria Beatrice Del Signore, Francesco Carimi, Angela Carra, Ada Ricci

Risultato della ricerca: Article

13 Citazioni (Scopus)

Abstract

A protocol for in vitro multiplication of caper (Capparis spinosa L. subsp. rupestris) from nodal segments collected from mature plants was developed. For shoot multiplication, one auxin (indol-3-butyric acid, IBA) and cytokinins of two different classes were used: the N6-substituted adenine derivatives 6-benzylamino purine (BAP), and the two synthetic phenylurea derivatives Nphenyl-N0-benzothiazol-6-ylurea (PBU) and N-phenyl-N0-(1,2,3-thidiazol-5-yl) urea (thidiazuron, TDZ). Maximum shoot production was achieved from explants cultured with the adeninic cytokinin BAP (4 lM) and the auxin IBA (0.5 lM). New shoots longer than 1 cm were used for rooting. To induce root formation, three auxins [indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and 3-Indoleacetic acid (IAA)] and two synthetic phenylurea derivatives [N,N-bis-(2,3-methylenedioxyphenyl)urea (2,3-MDPU) and N,N-bis-(3,4-methylenedioxyphenyl)urea(3,4-MDPU)] were used. All rooting compounds tested stimulated the formation of roots. However, the best result in terms of a high percentage of rooted shoots having awell-developed root system with many lateral roots was achieved with the synthetic phenylurea 2,3-MDPU (1 lM) with 93.7% of well rooted plantlets. About 80% of rootedplantlets were successfully acclimatized and transferred to the greenhouse.
Lingua originaleEnglish
Numero di pagine0
RivistaPlant Growth Regulation
Stato di pubblicazionePublished - 2011

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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