Potential involvement of IL-9 and Th9 cells in the pathogenesis of rheumatoid arthritis

Francesco Ciccia, Riccardo Alessandro, Giuliana Guggino, Giovanni Triolo, Francesco Dieli, Guido Sireci, Giuliana Guggino, Giovanni Triolo, Aroldo Rizzo, Carlo Maurizio Montecucco, Carlo Maurizio Montecucco, Francesco Ciccia, Antonio Manzo, Barbara Vitolo

Risultato della ricerca: Article

40 Citazioni (Scopus)

Abstract

Objective. IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. Methods. IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4+ T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. Results. IL-9 was overexpressed in RA synovial tissues and correlated with the degree of histological organization of B and T cells in ectopic lymphoid structures. The majority of IL-9-producing cells were identified as CD3+ cells. Increased mRNA and protein expression of IL-9R, IL-4, TSLP and TGF-β was also observed in RA synovial tissue. Blood peripheral Th9 cells were expanded by citrullinated peptides. Conclusion. These results indicate that Th9 cells and IL-9 were frequently detected in peripheral blood mononuclear cells and synovia of RA patients. A possible pathogenic role for Th9 in RA is discussed.
Lingua originaleEnglish
pagine (da-a)2264-2272
Numero di pagine9
RivistaRheumatology
Volume54
Stato di pubblicazionePublished - 2015

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Interleukin-9
Rheumatoid Arthritis
Interleukin-4
Peptides
Synovial Fluid
T-Lymphocyte Subsets
Real-Time Polymerase Chain Reaction
Blood Cells
Flow Cytometry
B-Lymphocytes
Joints
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Pharmacology (medical)

Cita questo

Potential involvement of IL-9 and Th9 cells in the pathogenesis of rheumatoid arthritis. / Ciccia, Francesco; Alessandro, Riccardo; Guggino, Giuliana; Triolo, Giovanni; Dieli, Francesco; Sireci, Guido; Guggino, Giuliana; Triolo, Giovanni; Rizzo, Aroldo; Montecucco, Carlo Maurizio; Montecucco, Carlo Maurizio; Ciccia, Francesco; Manzo, Antonio; Vitolo, Barbara.

In: Rheumatology, Vol. 54, 2015, pag. 2264-2272.

Risultato della ricerca: Article

Ciccia, F, Alessandro, R, Guggino, G, Triolo, G, Dieli, F, Sireci, G, Guggino, G, Triolo, G, Rizzo, A, Montecucco, CM, Montecucco, CM, Ciccia, F, Manzo, A & Vitolo, B 2015, 'Potential involvement of IL-9 and Th9 cells in the pathogenesis of rheumatoid arthritis', Rheumatology, vol. 54, pagg. 2264-2272.
Ciccia, Francesco ; Alessandro, Riccardo ; Guggino, Giuliana ; Triolo, Giovanni ; Dieli, Francesco ; Sireci, Guido ; Guggino, Giuliana ; Triolo, Giovanni ; Rizzo, Aroldo ; Montecucco, Carlo Maurizio ; Montecucco, Carlo Maurizio ; Ciccia, Francesco ; Manzo, Antonio ; Vitolo, Barbara. / Potential involvement of IL-9 and Th9 cells in the pathogenesis of rheumatoid arthritis. In: Rheumatology. 2015 ; Vol. 54. pagg. 2264-2272.
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title = "Potential involvement of IL-9 and Th9 cells in the pathogenesis of rheumatoid arthritis",
abstract = "Objective. IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. Methods. IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4+ T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. Results. IL-9 was overexpressed in RA synovial tissues and correlated with the degree of histological organization of B and T cells in ectopic lymphoid structures. The majority of IL-9-producing cells were identified as CD3+ cells. Increased mRNA and protein expression of IL-9R, IL-4, TSLP and TGF-β was also observed in RA synovial tissue. Blood peripheral Th9 cells were expanded by citrullinated peptides. Conclusion. These results indicate that Th9 cells and IL-9 were frequently detected in peripheral blood mononuclear cells and synovia of RA patients. A possible pathogenic role for Th9 in RA is discussed.",
keywords = "Citrullinated peptide; IL-9; Rheumatoid arthritis; Th9 cells; Adolescent; Adult; Arthritis, Cultured; Cytokines; Female; Gene Expression Regulation; Humans; Interleukin-4; Interleukin-9; Lymphocyte Activation; Male; Middle Aged; RNA, Messenger; Synovial Membrane; T-Lymphocyte Subsets; Transforming Growth Factor beta; Young Adult; Rheumatology; Medicine (all); Pharmacology (medical), Rheumatoid; CD4-Positive T-Lymphocytes; Cells",
author = "Francesco Ciccia and Riccardo Alessandro and Giuliana Guggino and Giovanni Triolo and Francesco Dieli and Guido Sireci and Giuliana Guggino and Giovanni Triolo and Aroldo Rizzo and Montecucco, {Carlo Maurizio} and Montecucco, {Carlo Maurizio} and Francesco Ciccia and Antonio Manzo and Barbara Vitolo",
year = "2015",
language = "English",
volume = "54",
pages = "2264--2272",
journal = "Rheumatology",
issn = "1462-0324",
publisher = "Oxford University Press",

}

TY - JOUR

T1 - Potential involvement of IL-9 and Th9 cells in the pathogenesis of rheumatoid arthritis

AU - Ciccia, Francesco

AU - Alessandro, Riccardo

AU - Guggino, Giuliana

AU - Triolo, Giovanni

AU - Dieli, Francesco

AU - Sireci, Guido

AU - Guggino, Giuliana

AU - Triolo, Giovanni

AU - Rizzo, Aroldo

AU - Montecucco, Carlo Maurizio

AU - Montecucco, Carlo Maurizio

AU - Ciccia, Francesco

AU - Manzo, Antonio

AU - Vitolo, Barbara

PY - 2015

Y1 - 2015

N2 - Objective. IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. Methods. IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4+ T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. Results. IL-9 was overexpressed in RA synovial tissues and correlated with the degree of histological organization of B and T cells in ectopic lymphoid structures. The majority of IL-9-producing cells were identified as CD3+ cells. Increased mRNA and protein expression of IL-9R, IL-4, TSLP and TGF-β was also observed in RA synovial tissue. Blood peripheral Th9 cells were expanded by citrullinated peptides. Conclusion. These results indicate that Th9 cells and IL-9 were frequently detected in peripheral blood mononuclear cells and synovia of RA patients. A possible pathogenic role for Th9 in RA is discussed.

AB - Objective. IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. Methods. IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4+ T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. Results. IL-9 was overexpressed in RA synovial tissues and correlated with the degree of histological organization of B and T cells in ectopic lymphoid structures. The majority of IL-9-producing cells were identified as CD3+ cells. Increased mRNA and protein expression of IL-9R, IL-4, TSLP and TGF-β was also observed in RA synovial tissue. Blood peripheral Th9 cells were expanded by citrullinated peptides. Conclusion. These results indicate that Th9 cells and IL-9 were frequently detected in peripheral blood mononuclear cells and synovia of RA patients. A possible pathogenic role for Th9 in RA is discussed.

KW - Citrullinated peptide; IL-9; Rheumatoid arthritis; Th9 cells; Adolescent; Adult; Arthritis

KW - Cultured; Cytokines; Female; Gene Expression Regulation; Humans; Interleukin-4; Interleukin-9; Lymphocyte Activation; Male; Middle Aged; RNA

KW - Messenger; Synovial Membrane; T-Lymphocyte Subsets; Transforming Growth Factor beta; Young Adult; Rheumatology; Medicine (all); Pharmacology (medical)

KW - Rheumatoid; CD4-Positive T-Lymphocytes; Cells

UR - http://hdl.handle.net/10447/207045

UR - http://rheumatology.oxfordjournals.org/

M3 - Article

VL - 54

SP - 2264

EP - 2272

JO - Rheumatology

JF - Rheumatology

SN - 1462-0324

ER -