Postnatal development of the dopaminergic signaling involved in the modulation of intestinal motility in mice

Michelangelo Auteri, Flavia Mule', Mariangela Mastropaolo, Maria Grazia Zizzo, Rosa Maria Serio, Gaetano Felice Caldara, Domenico Nuzzo, Giacomo Cavallaro, Ilaria Amodeo, Marta Di Carlo, Monica Fumagalli, Fabio Mosca, Domenico Nuzzo, Marta Di Carlo

Risultato della ricerca: Article

5 Citazioni (Scopus)

Abstract

Background:Since antidopaminergic drugs are pharmacological agents employed in the management of gastrointestinal motor disorders at all ages, we investigated whether the enteric dopaminergic system may undergo developmental changes after birth.Methods:Intestinal mechanical activity was examined in vitro as changes in isometric tension.Results:In 2-d-old (P2) mice, dopamine induced a contractile effect, decreasing in intensity with age, replaced, at the weaning (day 20), by a relaxant response. Both responses were tetrodotoxin (TTX)-insensitive. In P2, dopaminergic contraction was inhibited by D1-like receptor antagonist and mimicked by D1-like receptor agonist. In 90-d-old (P90) mice, the relaxation was reduced by both D1- and D2-like receptor antagonists, and mimicked by D1- and D2-like receptor agonists. In P2, contraction was antagonized by phospholipase C inhibitor, while in P90 relaxation was antagonized by adenylyl cyclase inhibitor and potentiated by phospholipase C inhibitor. The presence of dopamine receptors was assessed by immunofluorescence. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed a significant increase in D1, D2, and D3 receptor expression in proximal intestine with the age.Conclusion:In mouse small intestine, the response to dopamine undergoes developmental changes shifting from contraction to relaxation at weaning, as the consequence of D2-like receptor recruitment and increased expression of D1 receptors.
Lingua originaleEnglish
pagine (da-a)440-447
Numero di pagine8
RivistaPediatric Research
Volume80
Stato di pubblicazionePublished - 2016

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Gastrointestinal Motility
Type C Phospholipases
Weaning
Dopamine
Tetrodotoxin
Dopamine Receptors
Small Intestine
Intestines
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Parturition
Pharmacology
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cita questo

Postnatal development of the dopaminergic signaling involved in the modulation of intestinal motility in mice. / Auteri, Michelangelo; Mule', Flavia; Mastropaolo, Mariangela; Zizzo, Maria Grazia; Serio, Rosa Maria; Caldara, Gaetano Felice; Nuzzo, Domenico; Cavallaro, Giacomo; Amodeo, Ilaria; Di Carlo, Marta; Fumagalli, Monica; Mosca, Fabio; Nuzzo, Domenico; Di Carlo, Marta.

In: Pediatric Research, Vol. 80, 2016, pag. 440-447.

Risultato della ricerca: Article

Auteri, M, Mule', F, Mastropaolo, M, Zizzo, MG, Serio, RM, Caldara, GF, Nuzzo, D, Cavallaro, G, Amodeo, I, Di Carlo, M, Fumagalli, M, Mosca, F, Nuzzo, D & Di Carlo, M 2016, 'Postnatal development of the dopaminergic signaling involved in the modulation of intestinal motility in mice', Pediatric Research, vol. 80, pagg. 440-447.
Auteri, Michelangelo ; Mule', Flavia ; Mastropaolo, Mariangela ; Zizzo, Maria Grazia ; Serio, Rosa Maria ; Caldara, Gaetano Felice ; Nuzzo, Domenico ; Cavallaro, Giacomo ; Amodeo, Ilaria ; Di Carlo, Marta ; Fumagalli, Monica ; Mosca, Fabio ; Nuzzo, Domenico ; Di Carlo, Marta. / Postnatal development of the dopaminergic signaling involved in the modulation of intestinal motility in mice. In: Pediatric Research. 2016 ; Vol. 80. pagg. 440-447.
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abstract = "Background:Since antidopaminergic drugs are pharmacological agents employed in the management of gastrointestinal motor disorders at all ages, we investigated whether the enteric dopaminergic system may undergo developmental changes after birth.Methods:Intestinal mechanical activity was examined in vitro as changes in isometric tension.Results:In 2-d-old (P2) mice, dopamine induced a contractile effect, decreasing in intensity with age, replaced, at the weaning (day 20), by a relaxant response. Both responses were tetrodotoxin (TTX)-insensitive. In P2, dopaminergic contraction was inhibited by D1-like receptor antagonist and mimicked by D1-like receptor agonist. In 90-d-old (P90) mice, the relaxation was reduced by both D1- and D2-like receptor antagonists, and mimicked by D1- and D2-like receptor agonists. In P2, contraction was antagonized by phospholipase C inhibitor, while in P90 relaxation was antagonized by adenylyl cyclase inhibitor and potentiated by phospholipase C inhibitor. The presence of dopamine receptors was assessed by immunofluorescence. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed a significant increase in D1, D2, and D3 receptor expression in proximal intestine with the age.Conclusion:In mouse small intestine, the response to dopamine undergoes developmental changes shifting from contraction to relaxation at weaning, as the consequence of D2-like receptor recruitment and increased expression of D1 receptors.",
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author = "Michelangelo Auteri and Flavia Mule' and Mariangela Mastropaolo and Zizzo, {Maria Grazia} and Serio, {Rosa Maria} and Caldara, {Gaetano Felice} and Domenico Nuzzo and Giacomo Cavallaro and Ilaria Amodeo and {Di Carlo}, Marta and Monica Fumagalli and Fabio Mosca and Domenico Nuzzo and {Di Carlo}, Marta",
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T1 - Postnatal development of the dopaminergic signaling involved in the modulation of intestinal motility in mice

AU - Auteri, Michelangelo

AU - Mule', Flavia

AU - Mastropaolo, Mariangela

AU - Zizzo, Maria Grazia

AU - Serio, Rosa Maria

AU - Caldara, Gaetano Felice

AU - Nuzzo, Domenico

AU - Cavallaro, Giacomo

AU - Amodeo, Ilaria

AU - Di Carlo, Marta

AU - Fumagalli, Monica

AU - Mosca, Fabio

AU - Nuzzo, Domenico

AU - Di Carlo, Marta

PY - 2016

Y1 - 2016

N2 - Background:Since antidopaminergic drugs are pharmacological agents employed in the management of gastrointestinal motor disorders at all ages, we investigated whether the enteric dopaminergic system may undergo developmental changes after birth.Methods:Intestinal mechanical activity was examined in vitro as changes in isometric tension.Results:In 2-d-old (P2) mice, dopamine induced a contractile effect, decreasing in intensity with age, replaced, at the weaning (day 20), by a relaxant response. Both responses were tetrodotoxin (TTX)-insensitive. In P2, dopaminergic contraction was inhibited by D1-like receptor antagonist and mimicked by D1-like receptor agonist. In 90-d-old (P90) mice, the relaxation was reduced by both D1- and D2-like receptor antagonists, and mimicked by D1- and D2-like receptor agonists. In P2, contraction was antagonized by phospholipase C inhibitor, while in P90 relaxation was antagonized by adenylyl cyclase inhibitor and potentiated by phospholipase C inhibitor. The presence of dopamine receptors was assessed by immunofluorescence. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed a significant increase in D1, D2, and D3 receptor expression in proximal intestine with the age.Conclusion:In mouse small intestine, the response to dopamine undergoes developmental changes shifting from contraction to relaxation at weaning, as the consequence of D2-like receptor recruitment and increased expression of D1 receptors.

AB - Background:Since antidopaminergic drugs are pharmacological agents employed in the management of gastrointestinal motor disorders at all ages, we investigated whether the enteric dopaminergic system may undergo developmental changes after birth.Methods:Intestinal mechanical activity was examined in vitro as changes in isometric tension.Results:In 2-d-old (P2) mice, dopamine induced a contractile effect, decreasing in intensity with age, replaced, at the weaning (day 20), by a relaxant response. Both responses were tetrodotoxin (TTX)-insensitive. In P2, dopaminergic contraction was inhibited by D1-like receptor antagonist and mimicked by D1-like receptor agonist. In 90-d-old (P90) mice, the relaxation was reduced by both D1- and D2-like receptor antagonists, and mimicked by D1- and D2-like receptor agonists. In P2, contraction was antagonized by phospholipase C inhibitor, while in P90 relaxation was antagonized by adenylyl cyclase inhibitor and potentiated by phospholipase C inhibitor. The presence of dopamine receptors was assessed by immunofluorescence. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed a significant increase in D1, D2, and D3 receptor expression in proximal intestine with the age.Conclusion:In mouse small intestine, the response to dopamine undergoes developmental changes shifting from contraction to relaxation at weaning, as the consequence of D2-like receptor recruitment and increased expression of D1 receptors.

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