Porous PLLA scaffolds are optimal substrates for internal colonization by A6 mesoangioblastsand immunocytochemical analyses

Claudio Luparello, Giuseppina Turturici, Gabriella Sconzo, Vincenzo La Carrubba, Valerio Maria Bartolo Brucato, Fabiana Geraci, Francesco Carfi' Pavia, Giuseppina Turturici, Carfì-Pavia, Luparello, La Carrubba, Fabiana Geraci, Brucato, Sconzo

Risultato della ricerca: Articlepeer review

8 Citazioni (Scopus)

Abstract

In the present paper, mouse mesoangioblasts were seeded onto bidimensional matrices andwithin three-dimensional porous scaffolds of poly(L-lactic acid) (PLLA), in the presence or absence oftype I collagen coating, observed under the scanning electron microscope, and tested for their adhesion,survival and proliferation. Immunolocalization of Hsp70, an abundant and ubiquitous intracellularprotein in these cells, was also performed in sectioned cell-containing scaffolds under the confocalfluorescence microscope to check whether "in situ" analysis of intracellular constituents was feasible.The data obtained show that PLLA films allow direct cell adhesion and represent an optimal supportfor cell growth, and that the internal surfaces of PLLA polymeric sponges can be colonized bymesoangioblasts which can be submitted to "in situ" confocal microscopic analyses for possiblemonitoring of time-dependent expression of differentiation markers.
Lingua originaleEnglish
pagine (da-a)873-879
Numero di pagine7
RivistaJournal of Biosciences
Stato di pubblicazionePublished - 2009

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1100.1100???

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