Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization

Matteo Levantino, Henning Tidow, Stephan Niebling, Diana Monteiro, Inokentijs Josts, Yunyun Gao, Yunyun Gao, Matteo Levantino

Risultato della ricerca: Articlepeer review

20 Citazioni (Scopus)


This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged.
Lingua originaleEnglish
pagine (da-a)667-672
Numero di pagine6
Stato di pubblicazionePublished - 2018

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1300.1303???
  • ???subjectarea.asjc.2500.2500???
  • ???subjectarea.asjc.3100.3104???


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