Perinatal alcohol intake, maternal behaviour and transgenerational inheritance of drug abuse; effects of drinking pattern and environmental enrichment

Risultato della ricerca: Other

Abstract

Alcohol drinking during pregnancy and post-partum is a major concern because of the persistent neurobehavioral deficitsin the offspring that include increased vulnerability to substance abuse (McMurray et al., 2008). Moreover, the pattern ofalcohol consumption accounts for specific neurobiological alterations that involve brain regions, that significantly overlapwith those involved in maternal care behaviour (Stuber et al., 2008; George et al. 2012). Rodent studies on environmentalenrichment have clearly demonstrated the capacity for non-drug modification of addiction-related behaviours (Nader et al.,2012).Thus, this study aimed at: (I) exploring the consequences of continuous vs. intermittent alcohol-drinking pattern onmaternal behaviour and offspring vulnerability to alcohol; (II) evaluating the effects of environmental enrichment on theoffspring behavioural response to alcohol.Female rats were given continuous- or intermittent (CA, IA), two-bottle choice with water and 20% alcohol along a12-week period. Alcohol drinking was suspended during mating and resumed from late gestation throughout lactation,when they were assessed for home-cage undisturbed maternal behaviour. In the adulthood, alcohol-exposed offspring,reared in either standard- or enriched- conditions, were assessed for alcohol consummatory behaviour in a free-choiceparadigm (10% alcohol and water) and tested for the deprivation effect. Moreover, they were tested for behaviouralreactivity in the open field; anxiety-like behaviour in the elevated plus maze; depressive-like behaviour in the forced swimtest; spatial learning and memory and cognitive flexibility in the Morris water maze during the drinking paradigm.Alcohol drinking was able to disrupt spontaneous maternal behaviour, more in IA-dams (p<0.001) than CA ones. In theoffspring, perinatal alcohol IA induced higher alcohol intake and a stronger deprivation effect, with respect to CA(p<0.01). Overall, perinatal alcohol exposure decreased behavioural reactivity. Indeed, perinatally CA- and IA-exposedgroups spent less time in the central area of the open field (p<0.001) and on the open arms of the elevated plus maze(p<0.01), and showed increased immobility in the forced swim test (p<0.01), when compared to controls; notably, perinatalIA-group displayed learning and memory impairment (p<0.05) and decreased cognitive flexibility (p<0.5) with respect tocontrols in the water maze. The environmental enrichment exerted a protective role, in perinatally IA-rats and controlstowards the deprivation effect (p<00.1). Moreover, the enriched rearing condition increased behavioural reactivity(p<0.01), decreased anxiety-like behaviour (p<0.001) in both CA and IA- groups, and improved spatial learning andmemory in IA group (p<0.01). No effect was observed on depressive-like behaviour and reversal learning.In conclusion, this study indicates for the first time that the pattern of alcohol consumption can be responsible for differentextents of maternal behaviour disruption and detrimental consequences in the offspring. Rearing conditions that promotehigh sensory, motor, affective and cognitive stimulations foster resilience to alcohol abuse and improve affective tone andcognitive function; nevertheless they cannot be sufficient to full recovery from harmful effects of perinatal alcoholexposure.McMurray et al. (2008) Neurotoxicol Teratol. 30(6):475-86.Stuber et al. (2008) Alcohol Clin Exp Res. 32(10):1714-20George et al. (2012) Proc Natl Acad Sci U S A.109(44):18156-61Nader et al. (2012) Neuropsychopharmacol. 37, 1579-1587
Lingua originaleEnglish
Numero di pagine0
Stato di pubblicazionePublished - 2015

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Maternal Behavior
Drinking
Substance-Related Disorders
Alcohols
Alcohol Drinking
Water
Consummatory Behavior
Anxiety
Reversal Learning
Water Deprivation
Pregnancy
Lactation
Alcoholism
Rodentia
Learning

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@conference{a77c5a5978c347ac9085c2318eb09ca5,
title = "Perinatal alcohol intake, maternal behaviour and transgenerational inheritance of drug abuse; effects of drinking pattern and environmental enrichment",
abstract = "Alcohol drinking during pregnancy and post-partum is a major concern because of the persistent neurobehavioral deficitsin the offspring that include increased vulnerability to substance abuse (McMurray et al., 2008). Moreover, the pattern ofalcohol consumption accounts for specific neurobiological alterations that involve brain regions, that significantly overlapwith those involved in maternal care behaviour (Stuber et al., 2008; George et al. 2012). Rodent studies on environmentalenrichment have clearly demonstrated the capacity for non-drug modification of addiction-related behaviours (Nader et al.,2012).Thus, this study aimed at: (I) exploring the consequences of continuous vs. intermittent alcohol-drinking pattern onmaternal behaviour and offspring vulnerability to alcohol; (II) evaluating the effects of environmental enrichment on theoffspring behavioural response to alcohol.Female rats were given continuous- or intermittent (CA, IA), two-bottle choice with water and 20{\%} alcohol along a12-week period. Alcohol drinking was suspended during mating and resumed from late gestation throughout lactation,when they were assessed for home-cage undisturbed maternal behaviour. In the adulthood, alcohol-exposed offspring,reared in either standard- or enriched- conditions, were assessed for alcohol consummatory behaviour in a free-choiceparadigm (10{\%} alcohol and water) and tested for the deprivation effect. Moreover, they were tested for behaviouralreactivity in the open field; anxiety-like behaviour in the elevated plus maze; depressive-like behaviour in the forced swimtest; spatial learning and memory and cognitive flexibility in the Morris water maze during the drinking paradigm.Alcohol drinking was able to disrupt spontaneous maternal behaviour, more in IA-dams (p<0.001) than CA ones. In theoffspring, perinatal alcohol IA induced higher alcohol intake and a stronger deprivation effect, with respect to CA(p<0.01). Overall, perinatal alcohol exposure decreased behavioural reactivity. Indeed, perinatally CA- and IA-exposedgroups spent less time in the central area of the open field (p<0.001) and on the open arms of the elevated plus maze(p<0.01), and showed increased immobility in the forced swim test (p<0.01), when compared to controls; notably, perinatalIA-group displayed learning and memory impairment (p<0.05) and decreased cognitive flexibility (p<0.5) with respect tocontrols in the water maze. The environmental enrichment exerted a protective role, in perinatally IA-rats and controlstowards the deprivation effect (p<00.1). Moreover, the enriched rearing condition increased behavioural reactivity(p<0.01), decreased anxiety-like behaviour (p<0.001) in both CA and IA- groups, and improved spatial learning andmemory in IA group (p<0.01). No effect was observed on depressive-like behaviour and reversal learning.In conclusion, this study indicates for the first time that the pattern of alcohol consumption can be responsible for differentextents of maternal behaviour disruption and detrimental consequences in the offspring. Rearing conditions that promotehigh sensory, motor, affective and cognitive stimulations foster resilience to alcohol abuse and improve affective tone andcognitive function; nevertheless they cannot be sufficient to full recovery from harmful effects of perinatal alcoholexposure.McMurray et al. (2008) Neurotoxicol Teratol. 30(6):475-86.Stuber et al. (2008) Alcohol Clin Exp Res. 32(10):1714-20George et al. (2012) Proc Natl Acad Sci U S A.109(44):18156-61Nader et al. (2012) Neuropsychopharmacol. 37, 1579-1587",
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year = "2015",
language = "English",

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TY - CONF

T1 - Perinatal alcohol intake, maternal behaviour and transgenerational inheritance of drug abuse; effects of drinking pattern and environmental enrichment

AU - Cannizzaro, Carla

AU - Brancato, Anna

PY - 2015

Y1 - 2015

N2 - Alcohol drinking during pregnancy and post-partum is a major concern because of the persistent neurobehavioral deficitsin the offspring that include increased vulnerability to substance abuse (McMurray et al., 2008). Moreover, the pattern ofalcohol consumption accounts for specific neurobiological alterations that involve brain regions, that significantly overlapwith those involved in maternal care behaviour (Stuber et al., 2008; George et al. 2012). Rodent studies on environmentalenrichment have clearly demonstrated the capacity for non-drug modification of addiction-related behaviours (Nader et al.,2012).Thus, this study aimed at: (I) exploring the consequences of continuous vs. intermittent alcohol-drinking pattern onmaternal behaviour and offspring vulnerability to alcohol; (II) evaluating the effects of environmental enrichment on theoffspring behavioural response to alcohol.Female rats were given continuous- or intermittent (CA, IA), two-bottle choice with water and 20% alcohol along a12-week period. Alcohol drinking was suspended during mating and resumed from late gestation throughout lactation,when they were assessed for home-cage undisturbed maternal behaviour. In the adulthood, alcohol-exposed offspring,reared in either standard- or enriched- conditions, were assessed for alcohol consummatory behaviour in a free-choiceparadigm (10% alcohol and water) and tested for the deprivation effect. Moreover, they were tested for behaviouralreactivity in the open field; anxiety-like behaviour in the elevated plus maze; depressive-like behaviour in the forced swimtest; spatial learning and memory and cognitive flexibility in the Morris water maze during the drinking paradigm.Alcohol drinking was able to disrupt spontaneous maternal behaviour, more in IA-dams (p<0.001) than CA ones. In theoffspring, perinatal alcohol IA induced higher alcohol intake and a stronger deprivation effect, with respect to CA(p<0.01). Overall, perinatal alcohol exposure decreased behavioural reactivity. Indeed, perinatally CA- and IA-exposedgroups spent less time in the central area of the open field (p<0.001) and on the open arms of the elevated plus maze(p<0.01), and showed increased immobility in the forced swim test (p<0.01), when compared to controls; notably, perinatalIA-group displayed learning and memory impairment (p<0.05) and decreased cognitive flexibility (p<0.5) with respect tocontrols in the water maze. The environmental enrichment exerted a protective role, in perinatally IA-rats and controlstowards the deprivation effect (p<00.1). Moreover, the enriched rearing condition increased behavioural reactivity(p<0.01), decreased anxiety-like behaviour (p<0.001) in both CA and IA- groups, and improved spatial learning andmemory in IA group (p<0.01). No effect was observed on depressive-like behaviour and reversal learning.In conclusion, this study indicates for the first time that the pattern of alcohol consumption can be responsible for differentextents of maternal behaviour disruption and detrimental consequences in the offspring. Rearing conditions that promotehigh sensory, motor, affective and cognitive stimulations foster resilience to alcohol abuse and improve affective tone andcognitive function; nevertheless they cannot be sufficient to full recovery from harmful effects of perinatal alcoholexposure.McMurray et al. (2008) Neurotoxicol Teratol. 30(6):475-86.Stuber et al. (2008) Alcohol Clin Exp Res. 32(10):1714-20George et al. (2012) Proc Natl Acad Sci U S A.109(44):18156-61Nader et al. (2012) Neuropsychopharmacol. 37, 1579-1587

AB - Alcohol drinking during pregnancy and post-partum is a major concern because of the persistent neurobehavioral deficitsin the offspring that include increased vulnerability to substance abuse (McMurray et al., 2008). Moreover, the pattern ofalcohol consumption accounts for specific neurobiological alterations that involve brain regions, that significantly overlapwith those involved in maternal care behaviour (Stuber et al., 2008; George et al. 2012). Rodent studies on environmentalenrichment have clearly demonstrated the capacity for non-drug modification of addiction-related behaviours (Nader et al.,2012).Thus, this study aimed at: (I) exploring the consequences of continuous vs. intermittent alcohol-drinking pattern onmaternal behaviour and offspring vulnerability to alcohol; (II) evaluating the effects of environmental enrichment on theoffspring behavioural response to alcohol.Female rats were given continuous- or intermittent (CA, IA), two-bottle choice with water and 20% alcohol along a12-week period. Alcohol drinking was suspended during mating and resumed from late gestation throughout lactation,when they were assessed for home-cage undisturbed maternal behaviour. In the adulthood, alcohol-exposed offspring,reared in either standard- or enriched- conditions, were assessed for alcohol consummatory behaviour in a free-choiceparadigm (10% alcohol and water) and tested for the deprivation effect. Moreover, they were tested for behaviouralreactivity in the open field; anxiety-like behaviour in the elevated plus maze; depressive-like behaviour in the forced swimtest; spatial learning and memory and cognitive flexibility in the Morris water maze during the drinking paradigm.Alcohol drinking was able to disrupt spontaneous maternal behaviour, more in IA-dams (p<0.001) than CA ones. In theoffspring, perinatal alcohol IA induced higher alcohol intake and a stronger deprivation effect, with respect to CA(p<0.01). Overall, perinatal alcohol exposure decreased behavioural reactivity. Indeed, perinatally CA- and IA-exposedgroups spent less time in the central area of the open field (p<0.001) and on the open arms of the elevated plus maze(p<0.01), and showed increased immobility in the forced swim test (p<0.01), when compared to controls; notably, perinatalIA-group displayed learning and memory impairment (p<0.05) and decreased cognitive flexibility (p<0.5) with respect tocontrols in the water maze. The environmental enrichment exerted a protective role, in perinatally IA-rats and controlstowards the deprivation effect (p<00.1). Moreover, the enriched rearing condition increased behavioural reactivity(p<0.01), decreased anxiety-like behaviour (p<0.001) in both CA and IA- groups, and improved spatial learning andmemory in IA group (p<0.01). No effect was observed on depressive-like behaviour and reversal learning.In conclusion, this study indicates for the first time that the pattern of alcohol consumption can be responsible for differentextents of maternal behaviour disruption and detrimental consequences in the offspring. Rearing conditions that promotehigh sensory, motor, affective and cognitive stimulations foster resilience to alcohol abuse and improve affective tone andcognitive function; nevertheless they cannot be sufficient to full recovery from harmful effects of perinatal alcoholexposure.McMurray et al. (2008) Neurotoxicol Teratol. 30(6):475-86.Stuber et al. (2008) Alcohol Clin Exp Res. 32(10):1714-20George et al. (2012) Proc Natl Acad Sci U S A.109(44):18156-61Nader et al. (2012) Neuropsychopharmacol. 37, 1579-1587

UR - http://hdl.handle.net/10447/148751

UR - http://congresso.sifweb.org/abs/526.pdf

M3 - Other

ER -