PEG-benzofulvene copolymer hydrogels for antibody delivery

Gaetano Giammona, Mariano Licciardi, Gaetano Giammona, Andrea Cappelli, Germano Giuliani, Luigi Feruglio, Salvatore Valenti, Mariano Licciardi, Mauro Di Stefano, Mario Grassi

Risultato della ricerca: Article

14 Citazioni (Scopus)

Abstract

Poly[monomethylnona(ethylene glycol) 1-methylene-3-(4-methylphenyl)-1H-indene-2-carboxylate] (poly-1b) a new polymer based on a PEG-functionalized benzofulvene macromonomer have been investigated as hydrogel-based material for complexation and release of immunoglobulin (IgG) at physiological mimicking conditions. The polymer ability to complex human IgG has been studied by preparing copolymer/protein complexes obtained by spontaneous protein interactions onto polymer hydrogel aggregates, and the protein release rate has been evaluated at physiological conditions. SEM analysis was used to visualize the copolymer/IgG aggregates and its microstructured deposition. Moreover, rheological studies performed at 37 ◦C allowed determining hydrogel mechanical properties. On the basis of these information and NMR transverse relaxation measurements, the estimation of hydrogel mesh size distribution was possible. Finally, biological studies performed with poly-1b aqueous dispersions showed no cytotoxic effect on MCF-7 cell line, suggesting potential biocompatibility features for this polymer and making this new polymer a good potential candidate for the production of drug delivery systems.
Lingua originaleEnglish
pagine (da-a)-
Numero di pagine8
RivistaInternational Journal of Pharmaceutics
Volume2010
Stato di pubblicazionePublished - 2010

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Hydrogels
Hydrogel
Polymers
Antibodies
Immunoglobulin G
Drug Delivery Systems
Immunoglobulins
Proteins
Cell Line

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cita questo

Giammona, G., Licciardi, M., Giammona, G., Cappelli, A., Giuliani, G., Feruglio, L., ... Grassi, M. (2010). PEG-benzofulvene copolymer hydrogels for antibody delivery. International Journal of Pharmaceutics, 2010, -.

PEG-benzofulvene copolymer hydrogels for antibody delivery. / Giammona, Gaetano; Licciardi, Mariano; Giammona, Gaetano; Cappelli, Andrea; Giuliani, Germano; Feruglio, Luigi; Valenti, Salvatore; Licciardi, Mariano; Di Stefano, Mauro; Grassi, Mario.

In: International Journal of Pharmaceutics, Vol. 2010, 2010, pag. -.

Risultato della ricerca: Article

Giammona, G, Licciardi, M, Giammona, G, Cappelli, A, Giuliani, G, Feruglio, L, Valenti, S, Licciardi, M, Di Stefano, M & Grassi, M 2010, 'PEG-benzofulvene copolymer hydrogels for antibody delivery', International Journal of Pharmaceutics, vol. 2010, pagg. -.
Giammona, Gaetano ; Licciardi, Mariano ; Giammona, Gaetano ; Cappelli, Andrea ; Giuliani, Germano ; Feruglio, Luigi ; Valenti, Salvatore ; Licciardi, Mariano ; Di Stefano, Mauro ; Grassi, Mario. / PEG-benzofulvene copolymer hydrogels for antibody delivery. In: International Journal of Pharmaceutics. 2010 ; Vol. 2010. pagg. -.
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abstract = "Poly[monomethylnona(ethylene glycol) 1-methylene-3-(4-methylphenyl)-1H-indene-2-carboxylate] (poly-1b) a new polymer based on a PEG-functionalized benzofulvene macromonomer have been investigated as hydrogel-based material for complexation and release of immunoglobulin (IgG) at physiological mimicking conditions. The polymer ability to complex human IgG has been studied by preparing copolymer/protein complexes obtained by spontaneous protein interactions onto polymer hydrogel aggregates, and the protein release rate has been evaluated at physiological conditions. SEM analysis was used to visualize the copolymer/IgG aggregates and its microstructured deposition. Moreover, rheological studies performed at 37 ◦C allowed determining hydrogel mechanical properties. On the basis of these information and NMR transverse relaxation measurements, the estimation of hydrogel mesh size distribution was possible. Finally, biological studies performed with poly-1b aqueous dispersions showed no cytotoxic effect on MCF-7 cell line, suggesting potential biocompatibility features for this polymer and making this new polymer a good potential candidate for the production of drug delivery systems.",
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AU - Giammona, Gaetano

AU - Licciardi, Mariano

AU - Giammona, Gaetano

AU - Cappelli, Andrea

AU - Giuliani, Germano

AU - Feruglio, Luigi

AU - Valenti, Salvatore

AU - Licciardi, Mariano

AU - Di Stefano, Mauro

AU - Grassi, Mario

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N2 - Poly[monomethylnona(ethylene glycol) 1-methylene-3-(4-methylphenyl)-1H-indene-2-carboxylate] (poly-1b) a new polymer based on a PEG-functionalized benzofulvene macromonomer have been investigated as hydrogel-based material for complexation and release of immunoglobulin (IgG) at physiological mimicking conditions. The polymer ability to complex human IgG has been studied by preparing copolymer/protein complexes obtained by spontaneous protein interactions onto polymer hydrogel aggregates, and the protein release rate has been evaluated at physiological conditions. SEM analysis was used to visualize the copolymer/IgG aggregates and its microstructured deposition. Moreover, rheological studies performed at 37 ◦C allowed determining hydrogel mechanical properties. On the basis of these information and NMR transverse relaxation measurements, the estimation of hydrogel mesh size distribution was possible. Finally, biological studies performed with poly-1b aqueous dispersions showed no cytotoxic effect on MCF-7 cell line, suggesting potential biocompatibility features for this polymer and making this new polymer a good potential candidate for the production of drug delivery systems.

AB - Poly[monomethylnona(ethylene glycol) 1-methylene-3-(4-methylphenyl)-1H-indene-2-carboxylate] (poly-1b) a new polymer based on a PEG-functionalized benzofulvene macromonomer have been investigated as hydrogel-based material for complexation and release of immunoglobulin (IgG) at physiological mimicking conditions. The polymer ability to complex human IgG has been studied by preparing copolymer/protein complexes obtained by spontaneous protein interactions onto polymer hydrogel aggregates, and the protein release rate has been evaluated at physiological conditions. SEM analysis was used to visualize the copolymer/IgG aggregates and its microstructured deposition. Moreover, rheological studies performed at 37 ◦C allowed determining hydrogel mechanical properties. On the basis of these information and NMR transverse relaxation measurements, the estimation of hydrogel mesh size distribution was possible. Finally, biological studies performed with poly-1b aqueous dispersions showed no cytotoxic effect on MCF-7 cell line, suggesting potential biocompatibility features for this polymer and making this new polymer a good potential candidate for the production of drug delivery systems.

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