Patterns of K-ras mutation in colorectal carcinomas from Iran and Italy (a Gruppo Oncologico dell'Italia Meridionale study): influence of microsatellite instability status and country of origin.

Bishehsari, F.; Mahdavinia, M.; Malekzadeh, R.; Verginelli, F.; Catalano, T.; Sotoudeh, M.; Esposito, D.; De Lellis, L.; Semeraro, D.; Colucci, G.; Hormazdi, M.; Rakhshani, N.; Cama, A.; Piantelli, M.; Iacobelli, S.; Mariani Costantini, R.

Risultato della ricerca: Article

29 Citazioni (Scopus)

Abstract

Background: K-ras mutations are a key step in colorectal cancer progression. Such mutations have been widely studied in case series from Western countries but there are few data on the rate and spectrum of mutations in tumors from countries where the epidemiological features of the disease are different. Patients and methods: Tumor samples from 182 Iranian colorectal cancer patients (170 sporadic cases and 12 HNPCC cases) were screened for K-ras mutations at codons 12, 13 and 61 by sequencing analysis. The cases were also characterized for microsatellite instability at mononucleotide repeats by PCR and fragment analysis, and classified according to microsatellite instability status. The frequency and the spectrum of K-ras mutations were compared with those observed in a series of colorectal cancer patients from Italy. Results: K-ras mutations were observed in 68/182 (37.4%) cases. Mutation frequencies were similar in HNPCC-associated, sporadic MSI-H and sporadic microsatellite-stable (MSS) tumors. However, the G13D substitution was more frequent in HNPCC (3/ 4, 75%) and sporadic MSI- H (7/11, 63.6%) tumors compared to sporadic MSS tumors (11/ 53, 20.4%) (P < 0.01). Comparison of mutations in the two series from Iran and Italy showed a significantly higher frequency of G13D among Italian patients. Conclusions: While the frequency of K-ras mutations could be similar, the mutational spectrum could be differentially influenced by genetic and environmental factors.
Lingua originaleEnglish
pagine (da-a)91-96
Numero di pagine6
RivistaAnnals of Oncology
Volume17
Stato di pubblicazionePublished - 2006

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology

Cita questo

Bishehsari, F.; Mahdavinia, M.; Malekzadeh, R.; Verginelli, F.; Catalano, T.; Sotoudeh, M.; Esposito, D.; De Lellis, L.; Semeraro, D.; Colucci, G.; Hormazdi, M.; Rakhshani, N.; Cama, A.; Piantelli, M.; Iacobelli, S.; Mariani Costantini, R. (2006). Patterns of K-ras mutation in colorectal carcinomas from Iran and Italy (a Gruppo Oncologico dell'Italia Meridionale study): influence of microsatellite instability status and country of origin. Annals of Oncology, 17, 91-96.

Patterns of K-ras mutation in colorectal carcinomas from Iran and Italy (a Gruppo Oncologico dell'Italia Meridionale study): influence of microsatellite instability status and country of origin. / Bishehsari, F.; Mahdavinia, M.; Malekzadeh, R.; Verginelli, F.; Catalano, T.; Sotoudeh, M.; Esposito, D.; De Lellis, L.; Semeraro, D.; Colucci, G.; Hormazdi, M.; Rakhshani, N.; Cama, A.; Piantelli, M.; Iacobelli, S.; Mariani Costantini, R.

In: Annals of Oncology, Vol. 17, 2006, pag. 91-96.

Risultato della ricerca: Article

Bishehsari, F.; Mahdavinia, M.; Malekzadeh, R.; Verginelli, F.; Catalano, T.; Sotoudeh, M.; Esposito, D.; De Lellis, L.; Semeraro, D.; Colucci, G.; Hormazdi, M.; Rakhshani, N.; Cama, A.; Piantelli, M.; Iacobelli, S.; Mariani Costantini, R. 2006, 'Patterns of K-ras mutation in colorectal carcinomas from Iran and Italy (a Gruppo Oncologico dell'Italia Meridionale study): influence of microsatellite instability status and country of origin.', Annals of Oncology, vol. 17, pagg. 91-96.
Bishehsari, F.; Mahdavinia, M.; Malekzadeh, R.; Verginelli, F.; Catalano, T.; Sotoudeh, M.; Esposito, D.; De Lellis, L.; Semeraro, D.; Colucci, G.; Hormazdi, M.; Rakhshani, N.; Cama, A.; Piantelli, M.; Iacobelli, S.; Mariani Costantini, R. Patterns of K-ras mutation in colorectal carcinomas from Iran and Italy (a Gruppo Oncologico dell'Italia Meridionale study): influence of microsatellite instability status and country of origin. Annals of Oncology. 2006;17:91-96.
Bishehsari, F.; Mahdavinia, M.; Malekzadeh, R.; Verginelli, F.; Catalano, T.; Sotoudeh, M.; Esposito, D.; De Lellis, L.; Semeraro, D.; Colucci, G.; Hormazdi, M.; Rakhshani, N.; Cama, A.; Piantelli, M.; Iacobelli, S.; Mariani Costantini, R. / Patterns of K-ras mutation in colorectal carcinomas from Iran and Italy (a Gruppo Oncologico dell'Italia Meridionale study): influence of microsatellite instability status and country of origin. In: Annals of Oncology. 2006 ; Vol. 17. pagg. 91-96.
@article{e4d640e1eddd454ab4cef298f876a109,
title = "Patterns of K-ras mutation in colorectal carcinomas from Iran and Italy (a Gruppo Oncologico dell'Italia Meridionale study): influence of microsatellite instability status and country of origin.",
abstract = "Background: K-ras mutations are a key step in colorectal cancer progression. Such mutations have been widely studied in case series from Western countries but there are few data on the rate and spectrum of mutations in tumors from countries where the epidemiological features of the disease are different. Patients and methods: Tumor samples from 182 Iranian colorectal cancer patients (170 sporadic cases and 12 HNPCC cases) were screened for K-ras mutations at codons 12, 13 and 61 by sequencing analysis. The cases were also characterized for microsatellite instability at mononucleotide repeats by PCR and fragment analysis, and classified according to microsatellite instability status. The frequency and the spectrum of K-ras mutations were compared with those observed in a series of colorectal cancer patients from Italy. Results: K-ras mutations were observed in 68/182 (37.4{\%}) cases. Mutation frequencies were similar in HNPCC-associated, sporadic MSI-H and sporadic microsatellite-stable (MSS) tumors. However, the G13D substitution was more frequent in HNPCC (3/ 4, 75{\%}) and sporadic MSI- H (7/11, 63.6{\%}) tumors compared to sporadic MSS tumors (11/ 53, 20.4{\%}) (P < 0.01). Comparison of mutations in the two series from Iran and Italy showed a significantly higher frequency of G13D among Italian patients. Conclusions: While the frequency of K-ras mutations could be similar, the mutational spectrum could be differentially influenced by genetic and environmental factors.",
author = "{Bishehsari, F.; Mahdavinia, M.; Malekzadeh, R.; Verginelli, F.; Catalano, T.; Sotoudeh, M.; Esposito, D.; De Lellis, L.; Semeraro, D.; Colucci, G.; Hormazdi, M.; Rakhshani, N.; Cama, A.; Piantelli, M.; Iacobelli, S.; Mariani Costantini, R.} and Antonio Russo and Viviana Bazan and Valentina Agnese",
year = "2006",
language = "English",
volume = "17",
pages = "91--96",
journal = "Annals of Oncology",
issn = "0923-7534",
publisher = "Oxford University Press",

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TY - JOUR

T1 - Patterns of K-ras mutation in colorectal carcinomas from Iran and Italy (a Gruppo Oncologico dell'Italia Meridionale study): influence of microsatellite instability status and country of origin.

AU - Bishehsari, F.; Mahdavinia, M.; Malekzadeh, R.; Verginelli, F.; Catalano, T.; Sotoudeh, M.; Esposito, D.; De Lellis, L.; Semeraro, D.; Colucci, G.; Hormazdi, M.; Rakhshani, N.; Cama, A.; Piantelli, M.; Iacobelli, S.; Mariani

AU - Russo, Antonio

AU - Bazan, Viviana

AU - Agnese, Valentina

PY - 2006

Y1 - 2006

N2 - Background: K-ras mutations are a key step in colorectal cancer progression. Such mutations have been widely studied in case series from Western countries but there are few data on the rate and spectrum of mutations in tumors from countries where the epidemiological features of the disease are different. Patients and methods: Tumor samples from 182 Iranian colorectal cancer patients (170 sporadic cases and 12 HNPCC cases) were screened for K-ras mutations at codons 12, 13 and 61 by sequencing analysis. The cases were also characterized for microsatellite instability at mononucleotide repeats by PCR and fragment analysis, and classified according to microsatellite instability status. The frequency and the spectrum of K-ras mutations were compared with those observed in a series of colorectal cancer patients from Italy. Results: K-ras mutations were observed in 68/182 (37.4%) cases. Mutation frequencies were similar in HNPCC-associated, sporadic MSI-H and sporadic microsatellite-stable (MSS) tumors. However, the G13D substitution was more frequent in HNPCC (3/ 4, 75%) and sporadic MSI- H (7/11, 63.6%) tumors compared to sporadic MSS tumors (11/ 53, 20.4%) (P < 0.01). Comparison of mutations in the two series from Iran and Italy showed a significantly higher frequency of G13D among Italian patients. Conclusions: While the frequency of K-ras mutations could be similar, the mutational spectrum could be differentially influenced by genetic and environmental factors.

AB - Background: K-ras mutations are a key step in colorectal cancer progression. Such mutations have been widely studied in case series from Western countries but there are few data on the rate and spectrum of mutations in tumors from countries where the epidemiological features of the disease are different. Patients and methods: Tumor samples from 182 Iranian colorectal cancer patients (170 sporadic cases and 12 HNPCC cases) were screened for K-ras mutations at codons 12, 13 and 61 by sequencing analysis. The cases were also characterized for microsatellite instability at mononucleotide repeats by PCR and fragment analysis, and classified according to microsatellite instability status. The frequency and the spectrum of K-ras mutations were compared with those observed in a series of colorectal cancer patients from Italy. Results: K-ras mutations were observed in 68/182 (37.4%) cases. Mutation frequencies were similar in HNPCC-associated, sporadic MSI-H and sporadic microsatellite-stable (MSS) tumors. However, the G13D substitution was more frequent in HNPCC (3/ 4, 75%) and sporadic MSI- H (7/11, 63.6%) tumors compared to sporadic MSS tumors (11/ 53, 20.4%) (P < 0.01). Comparison of mutations in the two series from Iran and Italy showed a significantly higher frequency of G13D among Italian patients. Conclusions: While the frequency of K-ras mutations could be similar, the mutational spectrum could be differentially influenced by genetic and environmental factors.

UR - http://hdl.handle.net/10447/5601

M3 - Article

VL - 17

SP - 91

EP - 96

JO - Annals of Oncology

JF - Annals of Oncology

SN - 0923-7534

ER -