TY - JOUR
T1 - PARTIAL SEQUENCING OF THE BETA-GLUCOSIDASE-ENCODING GENE FROM YEAST STRAINS ISOLATED FROM MUSTS AND WINES
AU - Quatrini, Paola
AU - Puglia, Anna Maria
AU - Restuccia, Cristina
AU - Caggia, Cinzia
AU - Randazzo, Cinzia Lucia
AU - Palmeri, Rosa
AU - Barbagallo, Riccardo Nunzio
AU - Giudici, Paolo
AU - Spagna, Giovanni
AU - Marineo, Sandra
PY - 2008
Y1 - 2008
N2 - The aim of the present work was the identification of the gene encoding for β-glucosidase and its partial sequencing in the strainsPichia anomala AL112,Hanseniaspora uvarum Y8 andSaccharomyces cerevisiae AL41. To this aim degenerated primers, designed on the basis of aminoacid similarities of four known yeast β-glucosidases, have been used in PCR amplifications. An expected fragment of about 200 bp was amplified from all the DNAs, cloned and sequenced. Sequence homology demonstrated for the first time the presence of a β-glucosidase encoding gene inHanseniaspora uvarum andSaccharomyces cerevisiae.
AB - The aim of the present work was the identification of the gene encoding for β-glucosidase and its partial sequencing in the strainsPichia anomala AL112,Hanseniaspora uvarum Y8 andSaccharomyces cerevisiae AL41. To this aim degenerated primers, designed on the basis of aminoacid similarities of four known yeast β-glucosidases, have been used in PCR amplifications. An expected fragment of about 200 bp was amplified from all the DNAs, cloned and sequenced. Sequence homology demonstrated for the first time the presence of a β-glucosidase encoding gene inHanseniaspora uvarum andSaccharomyces cerevisiae.
KW - Hanseniaspora uvarum
KW - Pichia anomala
KW - Saccharomyces cerevisiae
KW - gene partial sequencing
KW - β-glucosidase
KW - Hanseniaspora uvarum
KW - Pichia anomala
KW - Saccharomyces cerevisiae
KW - gene partial sequencing
KW - β-glucosidase
UR - http://hdl.handle.net/10447/45863
M3 - Article
VL - 58
SP - 503
EP - 508
JO - Annals of Microbiology
JF - Annals of Microbiology
SN - 1590-4261
ER -