PARTIAL SEQUENCING OF THE BETA-GLUCOSIDASE-ENCODING GENE FROM YEAST STRAINS ISOLATED FROM MUSTS AND WINES

Paola Quatrini; Anna Maria Puglia; Cristina Restuccia; Cinzia Caggia; Cinzia Lucia Randazzo; Rosa Palmeri; Riccardo Nunzio Barbagallo; Paolo Giudici; Giovanni Spagna; Sandra Marineo

PARTIAL SEQUENCING OF THE BETA-GLUCOSIDASE-ENCODING GENE FROM YEAST STRAINS ISOLATED FROM MUSTS AND WINES

Paola Quatrini, Anna Maria Puglia, Cristina Restuccia, Cinzia Caggia, Cinzia Lucia Randazzo, Rosa Palmeri, Riccardo Nunzio Barbagallo, Paolo Giudici, Giovanni Spagna, Sandra Marineo

Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche

Risultato della ricerca: Article

10 Citazioni (Scopus)

Abstract

The aim of the present work was the identification of the gene encoding for β-glucosidase and its partial sequencing in the strainsPichia anomala AL112,Hanseniaspora uvarum Y8 andSaccharomyces cerevisiae AL41. To this aim degenerated primers, designed on the basis of aminoacid similarities of four known yeast β-glucosidases, have been used in PCR amplifications. An expected fragment of about 200 bp was amplified from all the DNAs, cloned and sequenced. Sequence homology demonstrated for the first time the presence of a β-glucosidase encoding gene inHanseniaspora uvarum andSaccharomyces cerevisiae.

Lingua originale	English
pagine (da-a)	503-508
Numero di pagine	6
Rivista	Annals of Microbiology
Volume	58
Stato di pubblicazione	Published - 2008

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All Science Journal Classification (ASJC) codes

Applied Microbiology and Biotechnology

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Quatrini, P., Puglia, A. M., Restuccia, C., Caggia, C., Randazzo, C. L., Palmeri, R., Barbagallo, R. N., Giudici, P., Spagna, G., & Marineo, S. (2008). PARTIAL SEQUENCING OF THE BETA-GLUCOSIDASE-ENCODING GENE FROM YEAST STRAINS ISOLATED FROM MUSTS AND WINES. Annals of Microbiology, 58, 503-508.

PARTIAL SEQUENCING OF THE BETA-GLUCOSIDASE-ENCODING GENE FROM YEAST STRAINS ISOLATED FROM MUSTS AND WINES. / Quatrini, Paola ; Puglia, Anna Maria; Restuccia, Cristina; Caggia, Cinzia; Randazzo, Cinzia Lucia; Palmeri, Rosa; Barbagallo, Riccardo Nunzio; Giudici, Paolo; Spagna, Giovanni; Marineo, Sandra.

In: Annals of Microbiology, Vol. 58, 2008, pag. 503-508.

Risultato della ricerca: Article

Quatrini, Paola ; Puglia, Anna Maria ; Restuccia, Cristina ; Caggia, Cinzia ; Randazzo, Cinzia Lucia ; Palmeri, Rosa ; Barbagallo, Riccardo Nunzio ; Giudici, Paolo ; Spagna, Giovanni ; Marineo, Sandra. / PARTIAL SEQUENCING OF THE BETA-GLUCOSIDASE-ENCODING GENE FROM YEAST STRAINS ISOLATED FROM MUSTS AND WINES. In: Annals of Microbiology. 2008 ; Vol. 58. pagg. 503-508.

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abstract = "The aim of the present work was the identification of the gene encoding for β-glucosidase and its partial sequencing in the strainsPichia anomala AL112,Hanseniaspora uvarum Y8 andSaccharomyces cerevisiae AL41. To this aim degenerated primers, designed on the basis of aminoacid similarities of four known yeast β-glucosidases, have been used in PCR amplifications. An expected fragment of about 200 bp was amplified from all the DNAs, cloned and sequenced. Sequence homology demonstrated for the first time the presence of a β-glucosidase encoding gene inHanseniaspora uvarum andSaccharomyces cerevisiae.",

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AU - Quatrini, Paola

AU - Puglia, Anna Maria

AU - Restuccia, Cristina

AU - Caggia, Cinzia

AU - Randazzo, Cinzia Lucia

AU - Palmeri, Rosa

AU - Barbagallo, Riccardo Nunzio

AU - Giudici, Paolo

AU - Spagna, Giovanni

AU - Marineo, Sandra

PY - 2008

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N2 - The aim of the present work was the identification of the gene encoding for β-glucosidase and its partial sequencing in the strainsPichia anomala AL112,Hanseniaspora uvarum Y8 andSaccharomyces cerevisiae AL41. To this aim degenerated primers, designed on the basis of aminoacid similarities of four known yeast β-glucosidases, have been used in PCR amplifications. An expected fragment of about 200 bp was amplified from all the DNAs, cloned and sequenced. Sequence homology demonstrated for the first time the presence of a β-glucosidase encoding gene inHanseniaspora uvarum andSaccharomyces cerevisiae.

AB - The aim of the present work was the identification of the gene encoding for β-glucosidase and its partial sequencing in the strainsPichia anomala AL112,Hanseniaspora uvarum Y8 andSaccharomyces cerevisiae AL41. To this aim degenerated primers, designed on the basis of aminoacid similarities of four known yeast β-glucosidases, have been used in PCR amplifications. An expected fragment of about 200 bp was amplified from all the DNAs, cloned and sequenced. Sequence homology demonstrated for the first time the presence of a β-glucosidase encoding gene inHanseniaspora uvarum andSaccharomyces cerevisiae.

KW - Hanseniaspora uvarum

KW - Pichia anomala

KW - Saccharomyces cerevisiae

KW - gene partial sequencing

KW - β-glucosidase

KW - Hanseniaspora uvarum

KW - Pichia anomala

KW - Saccharomyces cerevisiae

KW - gene partial sequencing

KW - β-glucosidase

UR - http://hdl.handle.net/10447/45863

M3 - Article

VL - 58

SP - 503

EP - 508

JO - Annals of Microbiology

JF - Annals of Microbiology

SN - 1590-4261

ER -

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