PARTIAL SEQUENCING OF THE BETA-GLUCOSIDASE-ENCODING GENE FROM YEAST STRAINS ISOLATED FROM MUSTS AND WINES

Paola Quatrini, Anna Maria Puglia, Cristina Restuccia, Cinzia Caggia, Cinzia Lucia Randazzo, Rosa Palmeri, Riccardo Nunzio Barbagallo, Paolo Giudici, Giovanni Spagna, Sandra Marineo

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10 Citazioni (Scopus)

Abstract

The aim of the present work was the identification of the gene encoding for β-glucosidase and its partial sequencing in the strainsPichia anomala AL112,Hanseniaspora uvarum Y8 andSaccharomyces cerevisiae AL41. To this aim degenerated primers, designed on the basis of aminoacid similarities of four known yeast β-glucosidases, have been used in PCR amplifications. An expected fragment of about 200 bp was amplified from all the DNAs, cloned and sequenced. Sequence homology demonstrated for the first time the presence of a β-glucosidase encoding gene inHanseniaspora uvarum andSaccharomyces cerevisiae.
Lingua originaleEnglish
pagine (da-a)503-508
Numero di pagine6
RivistaAnnals of Microbiology
Volume58
Stato di pubblicazionePublished - 2008

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All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology

Cita questo

Quatrini, P., Puglia, A. M., Restuccia, C., Caggia, C., Randazzo, C. L., Palmeri, R., Barbagallo, R. N., Giudici, P., Spagna, G., & Marineo, S. (2008). PARTIAL SEQUENCING OF THE BETA-GLUCOSIDASE-ENCODING GENE FROM YEAST STRAINS ISOLATED FROM MUSTS AND WINES. Annals of Microbiology, 58, 503-508.