Pancreatic islets from non-heart beating donor pig: Two-layer preservation method in an in vitro porcine model

Roberta Altomare, Francesco Cacciabaudo, Vincenzo Davide Palumbo, Giovanni Cassata, Giuseppe Damiano, Maria Concetta Gioviale, Attilio Ignazio Lo Monte, Giuseppe Damiano, Maurizio Bellavia, Giovanni Cassata, Francesco Cacciabaudo, Roberta Altomare, Roberto Puleio, Maria Concetta Gioviale, Attilio Ignazio Lo Monte, Vincenzo Davide Palumbo, Maurizio Bellavia

Risultato della ricerca: Articlepeer review

8 Citazioni (Scopus)

Abstract

Purpose: Pancreata from non-heart beating donors could represent an unlimited source of islets if their cell viability can be efficiently preserved during the time necessary to process the organs by the use of a better solution of preservation compared to the classic University of Wisconsin solution. The aim of this study was to determine whether it is possible to obtain functioning "alive islets" from non-heart-beating donors by comparing, on a porcine model, the classic "UW ice-store" method with a two-layer cold storage method (TLM) using oxygenated Perfluorocarbons (PFC) and UW.Methods: Whole pancreata were harvested from 20 NHBDs female pigs with similar characteristics and preserved for 4 h in UW solution (n=10) or TLM (UW/PFC) solution (n=10). The isolated islets were then evaluated for number, viability, purity, and insulin secretion, also estimated after 8 weeks of cryopreservation.Results: The total number of islets obtained from isolation, and their function assayed by the insulin stimulation index, before and after cryopreservation, showed a higher value in the TLM group. No significative differences in terms of purity and viability before and after cryopreservation were found when comparing the two groups.Conclusions: TLM solution for NHBDs porcine pancreata with cold ischemia time lower than 4 h offers significant advantages over UW solution storage, thereby increasing the isolation yield and isolation success rate of the pancreatic porcine islets
Lingua originaleEnglish
pagine (da-a)519-525
Numero di pagine7
RivistaInternational Journal of Artificial Organs
Volume34
Stato di pubblicazionePublished - 2011

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.2700.2701???
  • ???subjectarea.asjc.2500.2502???
  • ???subjectarea.asjc.2200.2204???

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