Oxygen Transfer Performances of Unbaffled Bio-Reactors with Various Aspect Ratios

Busciglio, A

Risultato della ricerca: Article

3 Citazioni (Scopus)

Abstract

Cultivation of microorganisms, plants or animal cells requires liquid agitation in order to ensure oxygen and nutrient transfer and to maintain cell suspension. Many studies on animal cell damage due to mechanical agitation and sparging aeration have shown that mechanical damage of freely suspended animal cells is in most cases associated with bursting bubbles at the air–liquid interface (Barrett et al., 2010). Gas bubbles are usually generated by direct air sparging aimed at supplying oxygen to the culture medium. Mechanical agitation may also introduce gas bubbles in the culture medium via vortexing entrainment from the free surface. In this work oxygen transfer performance of an unbaffled stirred bioreactor, with various aspect ratios, is presented in view of its use as a biochemical reactor for animal cell growth. In practice oxygen mass transfer occurs through the (more or less deep) free surface vortex which takes place when agitation is started in unbaffled vessels. If this vortex is not allowed to reach impeller blades, bubble formation and subsequent bursting at the free-surface is avoided. Experimental results show that this kind of bioreactor can provide sufficient oxygen mass transfer for animal cell growth, so resulting in a viable alternative to the more common sparged reactors. The mass transfer performance observed with the different aspect ratio configurations is also presented and discussed.
Lingua originaleEnglish
pagine (da-a)1-6
Numero di pagine6
RivistaChemical Engineering Transactions
Stato di pubblicazionePublished - 2014

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Aspect ratio
Animals
Oxygen
Mass transfer
Cells
Cell growth
Bioreactors
Bubbles (in fluids)
Culture Media
Vortex flow
Gases
Bubble formation
Liquids
Air
Microorganisms
Nutrients
Suspensions

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)

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title = "Oxygen Transfer Performances of Unbaffled Bio-Reactors with Various Aspect Ratios",
abstract = "Cultivation of microorganisms, plants or animal cells requires liquid agitation in order to ensure oxygen and nutrient transfer and to maintain cell suspension. Many studies on animal cell damage due to mechanical agitation and sparging aeration have shown that mechanical damage of freely suspended animal cells is in most cases associated with bursting bubbles at the air–liquid interface (Barrett et al., 2010). Gas bubbles are usually generated by direct air sparging aimed at supplying oxygen to the culture medium. Mechanical agitation may also introduce gas bubbles in the culture medium via vortexing entrainment from the free surface. In this work oxygen transfer performance of an unbaffled stirred bioreactor, with various aspect ratios, is presented in view of its use as a biochemical reactor for animal cell growth. In practice oxygen mass transfer occurs through the (more or less deep) free surface vortex which takes place when agitation is started in unbaffled vessels. If this vortex is not allowed to reach impeller blades, bubble formation and subsequent bursting at the free-surface is avoided. Experimental results show that this kind of bioreactor can provide sufficient oxygen mass transfer for animal cell growth, so resulting in a viable alternative to the more common sparged reactors. The mass transfer performance observed with the different aspect ratio configurations is also presented and discussed.",
author = "{Busciglio, A} and Franco Grisafi and Alberto Brucato and Micale, {Giorgio Domenico Maria} and Francesca Scargiali and Alessandro Tamburini",
year = "2014",
language = "English",
pages = "1--6",
journal = "Chemical Engineering Transactions",
issn = "2283-9216",
publisher = "AIDIC-Italian Association of Chemical Engineering",

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T1 - Oxygen Transfer Performances of Unbaffled Bio-Reactors with Various Aspect Ratios

AU - Busciglio, A

AU - Grisafi, Franco

AU - Brucato, Alberto

AU - Micale, Giorgio Domenico Maria

AU - Scargiali, Francesca

AU - Tamburini, Alessandro

PY - 2014

Y1 - 2014

N2 - Cultivation of microorganisms, plants or animal cells requires liquid agitation in order to ensure oxygen and nutrient transfer and to maintain cell suspension. Many studies on animal cell damage due to mechanical agitation and sparging aeration have shown that mechanical damage of freely suspended animal cells is in most cases associated with bursting bubbles at the air–liquid interface (Barrett et al., 2010). Gas bubbles are usually generated by direct air sparging aimed at supplying oxygen to the culture medium. Mechanical agitation may also introduce gas bubbles in the culture medium via vortexing entrainment from the free surface. In this work oxygen transfer performance of an unbaffled stirred bioreactor, with various aspect ratios, is presented in view of its use as a biochemical reactor for animal cell growth. In practice oxygen mass transfer occurs through the (more or less deep) free surface vortex which takes place when agitation is started in unbaffled vessels. If this vortex is not allowed to reach impeller blades, bubble formation and subsequent bursting at the free-surface is avoided. Experimental results show that this kind of bioreactor can provide sufficient oxygen mass transfer for animal cell growth, so resulting in a viable alternative to the more common sparged reactors. The mass transfer performance observed with the different aspect ratio configurations is also presented and discussed.

AB - Cultivation of microorganisms, plants or animal cells requires liquid agitation in order to ensure oxygen and nutrient transfer and to maintain cell suspension. Many studies on animal cell damage due to mechanical agitation and sparging aeration have shown that mechanical damage of freely suspended animal cells is in most cases associated with bursting bubbles at the air–liquid interface (Barrett et al., 2010). Gas bubbles are usually generated by direct air sparging aimed at supplying oxygen to the culture medium. Mechanical agitation may also introduce gas bubbles in the culture medium via vortexing entrainment from the free surface. In this work oxygen transfer performance of an unbaffled stirred bioreactor, with various aspect ratios, is presented in view of its use as a biochemical reactor for animal cell growth. In practice oxygen mass transfer occurs through the (more or less deep) free surface vortex which takes place when agitation is started in unbaffled vessels. If this vortex is not allowed to reach impeller blades, bubble formation and subsequent bursting at the free-surface is avoided. Experimental results show that this kind of bioreactor can provide sufficient oxygen mass transfer for animal cell growth, so resulting in a viable alternative to the more common sparged reactors. The mass transfer performance observed with the different aspect ratio configurations is also presented and discussed.

UR - http://hdl.handle.net/10447/97697

UR - http://www.aidic.it/cet/14/38/001.pdf

M3 - Article

SP - 1

EP - 6

JO - Chemical Engineering Transactions

JF - Chemical Engineering Transactions

SN - 2283-9216

ER -