Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions

Vito Chiantera, Sylvia Mechsner, Vito Chiantera, Maria Luisa Barcena De Arellano, Filiberto Vercellino, Julia Arnold, Achim Schneider

Risultato della ricerca: Article

50 Citazioni (Scopus)

Abstract

To investigate the role of the nerve growth factor (NGF) in the endometriosis-associated innervation in the development of endometriosis- associated symptoms, 41 peritoneal fluid samples (PF) from patients with surgically and histologically proven endometriosis and 20 PF from patients with other gynecologic conditions were analyzed with Western blot and a novel in vitro model using dorsal root ganglia (DRG) to show neuronal outgrowth; endometrial cells also were analyzed. The results suggest that the PF of endometriosis patients and endometriotic lesions have neurotropic properties, because the Western blot analysis and the cell culture stainings showed NGF expression, and the neurite outgrowth of DRG treated with PF of patients with endometriosis was significantly higher than when treated with PF of patients without endometriosis. Furthermore, blocking NGF with both anti-NGF and K252a leads to a significant decrease in neurite outgrowth. ©2011 by American Society for Reproductive Medicine.
Lingua originaleEnglish
pagine (da-a)1123-1126
Numero di pagine4
RivistaFertility and Sterility
Volume95
Stato di pubblicazionePublished - 2011

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Ascitic Fluid
Nerve Growth Factor
Endometriosis
Spinal Ganglia
Western Blotting
Neuronal Outgrowth
Cell Culture Techniques
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cita questo

Chiantera, V., Mechsner, S., Chiantera, V., Barcena De Arellano, M. L., Vercellino, F., Arnold, J., & Schneider, A. (2011). Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions. Fertility and Sterility, 95, 1123-1126.

Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions. / Chiantera, Vito; Mechsner, Sylvia; Chiantera, Vito; Barcena De Arellano, Maria Luisa; Vercellino, Filiberto; Arnold, Julia; Schneider, Achim.

In: Fertility and Sterility, Vol. 95, 2011, pag. 1123-1126.

Risultato della ricerca: Article

Chiantera, V, Mechsner, S, Chiantera, V, Barcena De Arellano, ML, Vercellino, F, Arnold, J & Schneider, A 2011, 'Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions', Fertility and Sterility, vol. 95, pagg. 1123-1126.
Chiantera, Vito ; Mechsner, Sylvia ; Chiantera, Vito ; Barcena De Arellano, Maria Luisa ; Vercellino, Filiberto ; Arnold, Julia ; Schneider, Achim. / Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions. In: Fertility and Sterility. 2011 ; Vol. 95. pagg. 1123-1126.
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abstract = "To investigate the role of the nerve growth factor (NGF) in the endometriosis-associated innervation in the development of endometriosis- associated symptoms, 41 peritoneal fluid samples (PF) from patients with surgically and histologically proven endometriosis and 20 PF from patients with other gynecologic conditions were analyzed with Western blot and a novel in vitro model using dorsal root ganglia (DRG) to show neuronal outgrowth; endometrial cells also were analyzed. The results suggest that the PF of endometriosis patients and endometriotic lesions have neurotropic properties, because the Western blot analysis and the cell culture stainings showed NGF expression, and the neurite outgrowth of DRG treated with PF of patients with endometriosis was significantly higher than when treated with PF of patients without endometriosis. Furthermore, blocking NGF with both anti-NGF and K252a leads to a significant decrease in neurite outgrowth. {\circledC}2011 by American Society for Reproductive Medicine.",
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T1 - Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions

AU - Chiantera, Vito

AU - Mechsner, Sylvia

AU - Chiantera, Vito

AU - Barcena De Arellano, Maria Luisa

AU - Vercellino, Filiberto

AU - Arnold, Julia

AU - Schneider, Achim

PY - 2011

Y1 - 2011

N2 - To investigate the role of the nerve growth factor (NGF) in the endometriosis-associated innervation in the development of endometriosis- associated symptoms, 41 peritoneal fluid samples (PF) from patients with surgically and histologically proven endometriosis and 20 PF from patients with other gynecologic conditions were analyzed with Western blot and a novel in vitro model using dorsal root ganglia (DRG) to show neuronal outgrowth; endometrial cells also were analyzed. The results suggest that the PF of endometriosis patients and endometriotic lesions have neurotropic properties, because the Western blot analysis and the cell culture stainings showed NGF expression, and the neurite outgrowth of DRG treated with PF of patients with endometriosis was significantly higher than when treated with PF of patients without endometriosis. Furthermore, blocking NGF with both anti-NGF and K252a leads to a significant decrease in neurite outgrowth. ©2011 by American Society for Reproductive Medicine.

AB - To investigate the role of the nerve growth factor (NGF) in the endometriosis-associated innervation in the development of endometriosis- associated symptoms, 41 peritoneal fluid samples (PF) from patients with surgically and histologically proven endometriosis and 20 PF from patients with other gynecologic conditions were analyzed with Western blot and a novel in vitro model using dorsal root ganglia (DRG) to show neuronal outgrowth; endometrial cells also were analyzed. The results suggest that the PF of endometriosis patients and endometriotic lesions have neurotropic properties, because the Western blot analysis and the cell culture stainings showed NGF expression, and the neurite outgrowth of DRG treated with PF of patients with endometriosis was significantly higher than when treated with PF of patients without endometriosis. Furthermore, blocking NGF with both anti-NGF and K252a leads to a significant decrease in neurite outgrowth. ©2011 by American Society for Reproductive Medicine.

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M3 - Article

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