Ovarian senescence increases liver fibrosis in humans and zebrafish with steatosis

Vincenza Calvaruso, Calogero Camma', Salvatore Petta, Antonio Craxi, Livia Maccio, Marcello Bianchini, Rosina Critelli, Elena Turola, Erica Villa, Fabiola Milosa, Antonio Grieco, Nazarena Raos, Marisa Di Giovanni, Serena Mercorella, Ester Vanni, Elisabetta Bugianesi, Franco Cotelli, Anna L. Fracanzani, Luca Valenti, Luca MieleSilvia Fargion

Risultato della ricerca: Article

20 Citazioni (Scopus)

Abstract

Contrasting data exist on the effect of gender and menopause on the susceptibility, development and liver damage progression in nonalcoholic fatty liver disease (NAFLD). Our aim was to assess whether menopause is associated with the severity of liver fibrosis in individuals with NAFLD and to explore the issue of ovarian senescence in experimental liver steatosis in zebrafish. In 244 females and age-matched males with biopsy-proven NAFLD, we assessed anthropometric, biochemical and metabolic features, including menopausal status (self-reported); liver biopsy was scored according to 'The Pathology Committee of the NASH Clinical Research Network'. Young and old male and female zebrafish were fed for 24 weeks with a high-calorie diet. Weekly body mass index (BMI), histopathological examination and quantitative real-time PCR analysis on genes involved in lipid metabolism, inflammation and fibrosis were performed. In the entire cohort, at multivariate logistic regression, male gender [odds ratio (OR): 1.408, 95% confidence interval (95% CI): 0.779-2.542, P=0.25] vs women at reproductive agewas not associated with F2-F4 fibrosis, whereas a trend was observed for menopause (OR: 1.752, 95% CI: 0.956-3.208, P=0.06). In women, menopause (OR: 2.717, 95% CI: 1.020-7.237, P=0.04) was independently associated with F2-F4 fibrosis. Similarly, in overfed zebrafish, old female fish with failing ovarian function [as demonstrated by extremely low circulating estradiol levels (1.4± 0.1 pg/μl) and prevailing presence of atretic follicles in the ovaries] developed massive steatosis and substantial fibrosis (comparable with that occurring in males), whereas young female fish developed less steatosis and were totally protected from the development of fibrosis. Ovarian senescence significantly increases the risk of fibrosis severity both in humans with NAFLD and in zebrafish with experimental steatosis.
Lingua originaleEnglish
pagine (da-a)1037-1046
Numero di pagine10
RivistaDISEASE MODELS & MECHANISMS
Volume8
Stato di pubblicazionePublished - 2015

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All Science Journal Classification (ASJC) codes

  • Neuroscience (miscellaneous)
  • Medicine (miscellaneous)
  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

Cita questo

Calvaruso, V., Camma', C., Petta, S., Craxi, A., Maccio, L., Bianchini, M., Critelli, R., Turola, E., Villa, E., Milosa, F., Grieco, A., Raos, N., Di Giovanni, M., Mercorella, S., Vanni, E., Bugianesi, E., Cotelli, F., Fracanzani, A. L., Valenti, L., ... Fargion, S. (2015). Ovarian senescence increases liver fibrosis in humans and zebrafish with steatosis. DISEASE MODELS & MECHANISMS, 8, 1037-1046.