OSTEOGENIC COMMITMENT AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS BY LOW-INTENSITY PULSED ULTRASOUND STIMULATION

Riccardo Alessandro, Francesca Monteleone, Simona Fontana, Stefania Setti, Maria Sartori, Angela De Luca, Valeria Carina, Viviana Costa, Cesare Faldini, Stefania Pagani, Gianluca Giavaresi, Milena Fini

Risultato della ricerca: Article

11 Citazioni (Scopus)

Abstract

Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis. LIPUS stimulation (a) regulated the balance between osteoblast commitment and differentiation by specific networks (activations of RhoA/ROCK signaling and upregulation of Ribosome constituent/Protein metabolic process, Glycolysis/Gluconeogenesis, RNA metabolic process/Splicing and Tubulins); (b) allowed the maintenance of a few percentage of osteoblast precursors (21d CD73 + /CD90 + : 6%; OCT-3/4 + /NANOG + /SOX2 + : 10%); (c) induced the activation of osteogenic specific pathways shown by gene expression (early: ALPL, COL1A1, late: RUNX2, BGLAP, MAPK1/6) and related protein release (COL1a1, OPN, OC), in particular in the presence of osteogenic soluble factors able to mimic bone microenvironment. To summarize, LIPUS might be able to improve the osteogenic commitment of hMSCs in vitro, and, at the same time, enhance their osteogenic differentiation. This article is protected by copyright. All rights reserved.
Lingua originaleEnglish
pagine (da-a)1558-1573
Numero di pagine16
RivistaJournal of Cellular Physiology
Volume233
Stato di pubblicazionePublished - 2017

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Stem cells
Mesenchymal Stromal Cells
Ultrasonics
Osteoblasts
Bone
Chemical activation
Maintenance
Bone and Bones
Gluconeogenesis
Glycolysis
Tubulin
Ribosomes
Gene expression
Proteomics
Proteins
Up-Regulation
Ultrasonic Waves
RNA
Gene Expression
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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OSTEOGENIC COMMITMENT AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS BY LOW-INTENSITY PULSED ULTRASOUND STIMULATION. / Alessandro, Riccardo; Monteleone, Francesca; Fontana, Simona; Setti, Stefania; Sartori, Maria; De Luca, Angela; Carina, Valeria; Costa, Viviana; Faldini, Cesare; Pagani, Stefania; Giavaresi, Gianluca; Fini, Milena.

In: Journal of Cellular Physiology, Vol. 233, 2017, pag. 1558-1573.

Risultato della ricerca: Article

Alessandro, R, Monteleone, F, Fontana, S, Setti, S, Sartori, M, De Luca, A, Carina, V, Costa, V, Faldini, C, Pagani, S, Giavaresi, G & Fini, M 2017, 'OSTEOGENIC COMMITMENT AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS BY LOW-INTENSITY PULSED ULTRASOUND STIMULATION', Journal of Cellular Physiology, vol. 233, pagg. 1558-1573.
Alessandro, Riccardo ; Monteleone, Francesca ; Fontana, Simona ; Setti, Stefania ; Sartori, Maria ; De Luca, Angela ; Carina, Valeria ; Costa, Viviana ; Faldini, Cesare ; Pagani, Stefania ; Giavaresi, Gianluca ; Fini, Milena. / OSTEOGENIC COMMITMENT AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS BY LOW-INTENSITY PULSED ULTRASOUND STIMULATION. In: Journal of Cellular Physiology. 2017 ; Vol. 233. pagg. 1558-1573.
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title = "OSTEOGENIC COMMITMENT AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS BY LOW-INTENSITY PULSED ULTRASOUND STIMULATION",
abstract = "Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis. LIPUS stimulation (a) regulated the balance between osteoblast commitment and differentiation by specific networks (activations of RhoA/ROCK signaling and upregulation of Ribosome constituent/Protein metabolic process, Glycolysis/Gluconeogenesis, RNA metabolic process/Splicing and Tubulins); (b) allowed the maintenance of a few percentage of osteoblast precursors (21d CD73 + /CD90 + : 6{\%}; OCT-3/4 + /NANOG + /SOX2 + : 10{\%}); (c) induced the activation of osteogenic specific pathways shown by gene expression (early: ALPL, COL1A1, late: RUNX2, BGLAP, MAPK1/6) and related protein release (COL1a1, OPN, OC), in particular in the presence of osteogenic soluble factors able to mimic bone microenvironment. To summarize, LIPUS might be able to improve the osteogenic commitment of hMSCs in vitro, and, at the same time, enhance their osteogenic differentiation. This article is protected by copyright. All rights reserved.",
author = "Riccardo Alessandro and Francesca Monteleone and Simona Fontana and Stefania Setti and Maria Sartori and {De Luca}, Angela and Valeria Carina and Viviana Costa and Cesare Faldini and Stefania Pagani and Gianluca Giavaresi and Milena Fini",
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T1 - OSTEOGENIC COMMITMENT AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS BY LOW-INTENSITY PULSED ULTRASOUND STIMULATION

AU - Alessandro, Riccardo

AU - Monteleone, Francesca

AU - Fontana, Simona

AU - Setti, Stefania

AU - Sartori, Maria

AU - De Luca, Angela

AU - Carina, Valeria

AU - Costa, Viviana

AU - Faldini, Cesare

AU - Pagani, Stefania

AU - Giavaresi, Gianluca

AU - Fini, Milena

PY - 2017

Y1 - 2017

N2 - Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis. LIPUS stimulation (a) regulated the balance between osteoblast commitment and differentiation by specific networks (activations of RhoA/ROCK signaling and upregulation of Ribosome constituent/Protein metabolic process, Glycolysis/Gluconeogenesis, RNA metabolic process/Splicing and Tubulins); (b) allowed the maintenance of a few percentage of osteoblast precursors (21d CD73 + /CD90 + : 6%; OCT-3/4 + /NANOG + /SOX2 + : 10%); (c) induced the activation of osteogenic specific pathways shown by gene expression (early: ALPL, COL1A1, late: RUNX2, BGLAP, MAPK1/6) and related protein release (COL1a1, OPN, OC), in particular in the presence of osteogenic soluble factors able to mimic bone microenvironment. To summarize, LIPUS might be able to improve the osteogenic commitment of hMSCs in vitro, and, at the same time, enhance their osteogenic differentiation. This article is protected by copyright. All rights reserved.

AB - Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis. LIPUS stimulation (a) regulated the balance between osteoblast commitment and differentiation by specific networks (activations of RhoA/ROCK signaling and upregulation of Ribosome constituent/Protein metabolic process, Glycolysis/Gluconeogenesis, RNA metabolic process/Splicing and Tubulins); (b) allowed the maintenance of a few percentage of osteoblast precursors (21d CD73 + /CD90 + : 6%; OCT-3/4 + /NANOG + /SOX2 + : 10%); (c) induced the activation of osteogenic specific pathways shown by gene expression (early: ALPL, COL1A1, late: RUNX2, BGLAP, MAPK1/6) and related protein release (COL1a1, OPN, OC), in particular in the presence of osteogenic soluble factors able to mimic bone microenvironment. To summarize, LIPUS might be able to improve the osteogenic commitment of hMSCs in vitro, and, at the same time, enhance their osteogenic differentiation. This article is protected by copyright. All rights reserved.

UR - http://hdl.handle.net/10447/234657

M3 - Article

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JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

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