On the Relationship between Jetted Inks and Printed Biopatterns:Molecular-Thin Functional Microarrays of Glucose Oxidase

Giuseppe Domenico Arrabito, Bruno Giuseppe Pignataro, Venera Aiello, Chiara Musumeci, Giuseppe Arrabito, Bruno Pignataro, Sebania Libertino, Giuseppe Compagnini

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28 Citazioni (Scopus)

Abstract

Arrays of circular spots of glucose oxidase have been obtained on functionalized silicon oxide by piezoelectric inkjet printing and the enzymatic activity toward glucose recognition has been monitored. The addition of glycerol to the molecular ink allows to obtain high spot definition and resolution (tens of micrometers wide; one molecule tall), but in spite of its well-known structural stabilizing properties, in dynamic conditions it may lead to increased protein stresses. The jetting voltage and pulse length have been found to be critical factors for both activity retention and pattern definition. High voltages and pulse lengths results in stress effects along with the loss of activity, which, at least in our experimental conditions, has been found to be recovered in time.
Lingua originaleEnglish
pagine (da-a)6312-6318
Numero di pagine7
RivistaLangmuir
Volume25
Stato di pubblicazionePublished - 2009

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All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cita questo

Arrabito, G. D., Pignataro, B. G., Aiello, V., Musumeci, C., Arrabito, G., Pignataro, B., Libertino, S., & Compagnini, G. (2009). On the Relationship between Jetted Inks and Printed Biopatterns:Molecular-Thin Functional Microarrays of Glucose Oxidase. Langmuir, 25, 6312-6318.